FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n02 z_rfp_26_feb.3420000000307e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n02 z_rfp_26_feb.3420000000307e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82419366
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC1261750.15308902036446143Illumina Single End PCR Primer 1 (95% over 22bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1056030.12812886718881095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG344100.031.65417343
CGGTGGT364100.029.63420944
GGTGGTC355450.029.15233645
CTCGGTG472450.023.00282542
TCTCGGT496150.022.35796541
AGATCTC537950.020.6086838
GATCTCG554950.020.2947939
ATCTCGG540250.020.19319340
GTAGATC577650.019.71866236
TAAGAGA363600.019.3041294
AGACAGC331600.019.155348
AAGAGAC344250.018.8291455
GAGACAG335550.018.6104287
GTATAAG593750.018.0455471
GACAGCT343400.018.0314359
ATAAGAG411050.017.9365313
TAGATCT671050.017.25931537
AGAGACA372900.017.0633686
GTGTAGA653400.017.05483434
AGTGTAG658450.016.72468233
Produced by FastQC (version 0.11.2)