FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n02 gdna_2000_dn_3_march.3420000000312d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n02 gdna_2000_dn_3_march.3420000000312d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31283147
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN394300.1260423064214096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT147000.018.74129745
CTGACGC211100.018.16737745
ACGGTAC52900.013.6418168
CCTTTGG186900.013.6322191
TCTGACG289550.013.24382544
CTTTGGG178600.013.144872
TAGACCG61400.013.0389165
ATGGTAC95700.012.5679578
ATAACGG70300.012.5502455
CCGTGCG32800.012.1152649
GGGAAAC201050.011.7256836
GGTACAA156800.011.660645510
AATTGAT217950.011.5850120
TTTGGGA205750.011.377813
ACCGTGC37300.011.2702378
TTGGGAA216300.011.2442154
GTCTAGT40500.010.8250841
TGCATAA176200.010.81210642
AGACCGT74050.010.7170376
ACATGAA239750.010.65371411
Produced by FastQC (version 0.11.2)