FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n02 dnrfp 24-feb.3420000000303b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n02 dnrfp 24-feb.3420000000303b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9872991
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN128130.12977830122604184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG21250.016.16682643
CTCGGTG21050.015.79054642
TCTCGGT20850.015.62095541
GTAGATC24100.013.31034236
GACGAGT24300.013.17638629
CTGCCGA24400.013.03847424
TGACGCT27900.012.99095619
ATCTCGG24600.012.94444440
AGATCTC24400.012.89169438
CTGACGC28450.012.73955518
CGGTGGT25750.012.73531544
CCGACGA25500.012.64358127
CGAGTGT27600.012.56884431
ACGAGTG27150.012.44866330
CGCTGCC28250.012.28545222
GGTGGTC26150.012.00894845
GACGCTG28900.011.93166920
GCTGCCG28000.011.75943623
TGCCGAC27850.011.66306425
TCTGACG30500.011.59161617
Produced by FastQC (version 0.11.2)