FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n02 dn_rfp_3_march.342000000030b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n02 dn_rfp_3_march.342000000030b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42496397
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC1312200.30877911838031824Illumina Single End PCR Primer 1 (95% over 22bp)
GTATAAGAGACAGCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTA791590.18627226209318404No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN530330.12479410901587729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG306100.036.0780443
CGGTGGT311800.035.15440444
GGTGGTC314050.033.97331245
GTATAAG278400.031.3297671
TATAAGA308900.028.304562
CTCGGTG410250.026.87015742
TCTCGGT416450.026.73768841
ATAAGAG302400.025.8010483
TAAGAGA306800.025.043644
GATCTCG466100.023.88633339
AGATCTC468100.023.49403638
ATCTCGG466100.023.18619340
AAGAGAC317900.023.111025
GTAGATC490250.022.7583936
AGACAGC342400.021.1187178
TAGATCT535000.020.92931237
GACAGCT341350.020.8386469
TGTAGAT545600.020.356135
GTGTAGA532650.020.32009134
AGTGTAG546850.019.87403933
Produced by FastQC (version 0.11.2)