FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n02 dn_rfp_26_feb.34200000003011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n02 dn_rfp_26_feb.34200000003011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6708917
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83940.12511706434883602No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG22150.024.36489143
CGGTGGT23400.022.20545244
GGTGGTC24250.021.48540545
CTCGGTG28100.018.65079542
TCTCGGT35050.015.58872641
AGATCTC37200.015.10413138
GTAGATC42400.013.86281836
GATCTCG41300.013.496613539
ATCTCGG40750.013.43594340
TAGACCG13600.013.0601565
GACGAGT44100.012.59822529
CCGACGA45800.012.37321627
GTGTAGA48900.012.15670434
TGCCGAC47450.011.99911725
TAGATCT50650.011.95646837
CGAGTGT46750.011.93243831
ACGAGTG46700.011.89682330
CTGCCGA45650.011.887609524
CGACGAG47800.011.85515328
AGTGTAG49100.011.69916533
Produced by FastQC (version 0.11.2)