##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H3NH5BCXX l02n01 z_rfp_26_feb.34100000003071.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82419366
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.932886780517094	38.0	32.0	38.0	27.0	38.0
2	34.83096509138398	38.0	32.0	38.0	32.0	38.0
3	34.809360096266694	38.0	32.0	38.0	32.0	38.0
4	35.05431919968906	38.0	32.0	38.0	32.0	38.0
5	35.04536005530545	38.0	32.0	38.0	32.0	38.0
6	36.82855945531054	38.0	38.0	40.0	32.0	40.0
7	36.639392300105776	38.0	38.0	40.0	32.0	40.0
8	36.558073826969256	38.0	38.0	40.0	32.0	40.0
9	36.52305689903026	38.0	38.0	40.0	32.0	40.0
10	36.453978643805634	38.0	38.0	40.0	32.0	40.0
11	36.48454727254272	38.0	38.0	40.0	32.0	40.0
12	36.416819804219315	38.0	38.0	40.0	32.0	40.0
13	36.38940130891082	38.0	38.0	40.0	32.0	40.0
14	36.34175040851443	38.0	38.0	40.0	32.0	40.0
15	36.341506278997585	38.0	38.0	40.0	32.0	40.0
16	36.35166112537192	38.0	38.0	40.0	32.0	40.0
17	36.34032875234687	38.0	38.0	40.0	32.0	40.0
18	36.319984528878805	38.0	38.0	40.0	32.0	40.0
19	36.295121258273205	38.0	38.0	40.0	32.0	40.0
20	36.291099569972424	38.0	38.0	40.0	32.0	40.0
21	36.25658229402056	38.0	38.0	40.0	32.0	40.0
22	36.23936353744823	38.0	38.0	40.0	32.0	40.0
23	36.21347338439852	38.0	38.0	40.0	32.0	40.0
24	36.19242495750331	38.0	38.0	40.0	32.0	40.0
25	36.1712009044088	38.0	38.0	40.0	32.0	40.0
26	36.026567239063695	38.0	38.0	40.0	27.0	40.0
27	35.95505225070525	38.0	38.0	40.0	27.0	40.0
28	35.92147464468484	38.0	38.0	40.0	27.0	40.0
29	35.90409743020833	38.0	38.0	40.0	27.0	40.0
30	35.856449975604036	38.0	38.0	40.0	27.0	40.0
31	35.83434565609252	38.0	38.0	40.0	27.0	40.0
32	35.82778373956432	38.0	38.0	40.0	27.0	40.0
33	35.80783046790241	38.0	38.0	40.0	27.0	40.0
34	35.75570171214372	38.0	38.0	40.0	27.0	40.0
35	35.72478407562611	38.0	38.0	40.0	27.0	40.0
36	35.70730093944183	38.0	38.0	40.0	27.0	40.0
37	35.680156712197956	38.0	38.0	40.0	27.0	40.0
38	35.64034020839228	38.0	38.0	40.0	27.0	40.0
39	35.61755476255423	38.0	38.0	40.0	27.0	40.0
40	35.595345686109745	38.0	38.0	40.0	27.0	40.0
41	35.5903475161408	38.0	38.0	40.0	27.0	40.0
42	35.56905429240987	38.0	38.0	40.0	27.0	40.0
43	35.543733107580564	38.0	38.0	40.0	27.0	40.0
44	35.45396549155692	38.0	38.0	40.0	27.0	40.0
45	35.424259754679504	38.0	38.0	40.0	27.0	40.0
46	35.42133815977182	38.0	38.0	40.0	27.0	40.0
47	35.37563652212515	38.0	38.0	40.0	27.0	40.0
48	35.34861366198813	38.0	38.0	40.0	27.0	40.0
49	35.3563173975398	38.0	38.0	40.0	27.0	40.0
50	35.32170670907612	38.0	38.0	40.0	27.0	40.0
51	34.74390427851629	38.0	32.0	38.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.9504054175105168
2201	2	0.23704536866552672
2201	3	0.27071958666503804
2201	4	-0.009340026712102656
2201	5	0.052936911433029366
2201	6	0.2142522207741635
2201	7	0.22985754306027673
2201	8	0.1811170895752241
2201	9	0.15315021153622865
2201	10	0.20317183554110585
2201	11	0.10151105441789099
2201	12	0.09736519881122518
2201	13	0.18965935886336638
2201	14	-0.012676403301043138
2201	15	-0.005095971472506733
2201	16	-0.023886337032486438
2201	17	-0.06907492410777394
2201	18	-0.11692297291965303
2201	19	-0.06563764144910778
2201	20	-0.001117426201396654
2201	21	0.1595127158740155
2201	22	0.15910647979546866
2201	23	-0.012325463627469446
2201	24	0.062324777833595135
2201	25	-0.030883054420158373
2201	26	0.11333790857219839
2201	27	-0.07467726143598696
2201	28	-0.06222703723185674
2201	29	-0.05791941320771343
2201	30	0.059317374872414064
2201	31	0.05779726068104907
2201	32	-0.012515150406514408
2201	33	0.030744190199683885
2201	34	-0.0710858285821061
2201	35	0.05684853932747558
2201	36	0.11227092808437789
2201	37	0.1577394837512074
2201	38	0.02711420064451886
2201	39	-0.08824948803337662
2201	40	-0.10988564622434183
2201	41	-0.008338492108748596
2201	42	-0.13396540943785595
2201	43	-0.11504755182861715
2201	44	-0.039415356691343106
2201	45	-0.0013176126363205753
2201	46	-0.13613329831465393
2201	47	-0.09236883764074832
2201	48	-0.18595857779070002
2201	49	-2.944649892384632E-4
2201	50	-0.08415127295614866
2201	51	-0.02305371005121515
1101	1	0.869757239444013
1101	2	0.2143723354405438
1101	3	0.14879963729985235
1101	4	0.06300210133373696
1101	5	0.13417141006797806
1101	6	0.1838086141786519
1101	7	0.18912321887894024
1101	8	0.18580783968059933
1101	9	0.16878775565177762
1101	10	0.16110251688861865
1101	11	0.14183722183629754
1101	12	0.06876246617741799
1101	13	0.057867826146349444
1101	14	0.07822101416597604
1101	15	0.11474232761715086
1101	16	0.10668953080515564
1101	17	0.08248487396703297
1101	18	0.10664214793558102
1101	19	0.07130714353827727
1101	20	-0.017103600958691345
1101	21	0.043721675665373994
1101	22	0.09363336593928295
1101	23	0.1084326815721397
1101	24	0.04477973851707162
1101	25	0.030450482161349157
1101	26	0.029370399972215466
1101	27	0.021470269241319784
1101	28	0.016053914928122026
1101	29	0.0659960104757431
1101	30	0.06650666645776226
1101	31	0.02491754619811104
1101	32	-0.027994311083858747
1101	33	0.02922023067504398
1101	34	0.05489236132383013
1101	35	0.019621166830191328
1101	36	-0.0035181281934839603
1101	37	-0.012846872244956842
1101	38	-0.027028825809452428
1101	39	-0.025562346267911096
1101	40	-0.03830357585267308
1101	41	-0.17793899942134317
1101	42	-0.11337705034478773
1101	43	-0.06614030180534058
1101	44	-0.18982825751715637
1101	45	-0.13198875941533572
1101	46	-0.10534083614614786
1101	47	-0.10512361197447717
1101	48	-0.20737041529891798
1101	49	-0.1684376254596316
1101	50	-0.22652580853131354
1101	51	-0.15208174106466288
2202	1	0.6334928003610827
2202	2	0.1272427360847388
2202	3	0.12164980330152986
2202	4	-0.02739102543697669
2202	5	0.13874571789731505
2202	6	0.13154993873921939
2202	7	0.13963462632252543
2202	8	0.08392876267355831
2202	9	-0.017240261080509356
2202	10	0.04392524296756051
2202	11	-0.09955196817166012
2202	12	0.0735293384831266
2202	13	-0.018226530832123444
2202	14	0.0960209436734587
2202	15	-0.025756379996892065
2202	16	-0.002678323049288167
2202	17	-0.012860959903001401
2202	18	-0.1173221928851973
2202	19	-0.07197492519537008
2202	20	-0.11029571767816293
2202	21	-0.09169753856978957
2202	22	-0.09300737042232754
2202	23	-0.009034872828053153
2202	24	-0.04288399477481164
2202	25	0.06712780694788734
2202	26	-0.03419889644798246
2202	27	-0.03717735260657662
2202	28	-0.0028090509218614557
2202	29	-0.07062992731825801
2202	30	-0.22100613370543698
2202	31	-0.1803472673293669
2202	32	-0.20578609738876708
2202	33	-0.13572144491028837
2202	34	-0.09098810342275243
2202	35	-0.0943061558732694
2202	36	-0.15161367518990687
2202	37	-0.06293315231526009
2202	38	-0.12529260579985646
2202	39	-0.24136337250756412
2202	40	-0.17801523377717388
2202	41	-0.13494650796976515
2202	42	-0.12886372176868832
2202	43	-0.31069248737777144
2202	44	-0.20590644314026463
2202	45	-0.2346694597981056
2202	46	-0.21312546445449243
2202	47	-0.23399838800142447
2202	48	-0.25021436001356534
2202	49	-0.2852035943133231
2202	50	-0.21718585449031735
2202	51	-0.34372728243084794
1102	1	0.722538170676259
1102	2	0.05399129485176246
1102	3	-0.017614338811995367
1102	4	-0.15298919705660552
1102	5	-0.08561564562250368
1102	6	-0.10606643527579251
1102	7	-0.15626875168121046
1102	8	-0.06118406592167247
1102	9	-0.1094491979586536
1102	10	-0.10134101516877791
1102	11	-0.16533983773388172
1102	12	-0.13458600449909142
1102	13	-0.20512813755956927
1102	14	-0.18773715491190757
1102	15	-0.16221020293122024
1102	16	-0.1719079249260247
1102	17	-0.16891528936087497
1102	18	-0.15810259918389136
1102	19	-0.2345259350469675
1102	20	-0.14857824616964166
1102	21	-0.16334106897858902
1102	22	-0.18672079823886634
1102	23	-0.18260390850176123
1102	24	-0.23304920238268068
1102	25	-0.21047117533611015
1102	26	-0.22327286738206453
1102	27	-0.2751555735600206
1102	28	-0.31197778078620786
1102	29	-0.29244489719192046
1102	30	-0.3476184993034437
1102	31	-0.35189402804407877
1102	32	-0.3482810101243743
1102	33	-0.31840930495067
1102	34	-0.33914833598114313
1102	35	-0.33409062108825793
1102	36	-0.40222768274467313
1102	37	-0.3272518338921131
1102	38	-0.4019053122947369
1102	39	-0.43591187139958265
1102	40	-0.36849289293115817
1102	41	-0.3574347414919643
1102	42	-0.46178028966100015
1102	43	-0.4486607083078056
1102	44	-0.4522564327503815
1102	45	-0.5303403256526238
1102	46	-0.483496882665861
1102	47	-0.506309181993835
1102	48	-0.6352391908445512
1102	49	-0.5602984133408881
1102	50	-0.5678280277200116
1102	51	-0.5194192285448196
2203	1	0.5595447125507675
2203	2	-0.08270048257396923
2203	3	-0.10525890400737836
2203	4	-0.22429840973531157
2203	5	-0.04283218024914248
2203	6	-0.28005697672999474
2203	7	-0.3608022931553805
2203	8	-0.5890605006528489
2203	9	-0.33042925816603486
2203	10	-0.27904779225053744
2203	11	-0.23740146982050447
2203	12	-0.22515096500250564
2203	13	-0.22595722622461523
2203	14	-0.20493077671208226
2203	15	-0.2439963012393136
2203	16	-0.28605907380886464
2203	17	-0.21951785796087364
2203	18	-0.21355483337904246
2203	19	-0.18660237147836511
2203	20	-0.2465403251860394
2203	21	-0.22516195773453518
2203	22	-0.196419821562543
2203	23	-0.3307532607308161
2203	24	-0.24455859876304942
2203	25	-0.18523603180669568
2203	26	-0.28859663512609046
2203	27	-0.2959251640849203
2203	28	-0.4004360154738791
2203	29	-0.4124320260499488
2203	30	-0.3285083383170715
2203	31	-0.30590625281522676
2203	32	-0.3539126521220268
2203	33	-0.3885713757143705
2203	34	-0.39443787919943674
2203	35	-0.3927662880837772
2203	36	-0.5032531037762809
2203	37	-0.45203284051402903
2203	38	-0.5309953301560526
2203	39	-0.435215671768141
2203	40	-0.3795603517776698
2203	41	-0.4268878284038138
2203	42	-0.4823077180792765
2203	43	-0.4338193760734441
2203	44	-0.5365593181958701
2203	45	-0.5208412871054904
2203	46	-0.421804864488422
2203	47	-0.485535919435371
2203	48	-0.5705642602620387
2203	49	-0.5320314923475777
2203	50	-0.5039838875428799
2203	51	-0.5913712434001326
1103	1	0.6047310792362168
1103	2	-0.018801576215551563
1103	3	-0.0817865934620059
1103	4	-0.18439757291030645
1103	5	-0.1905338871074278
1103	6	-0.11944609014329188
1103	7	-0.09360438728055698
1103	8	-0.12110795317808254
1103	9	-0.15301365159816527
1103	10	-0.17142681729113463
1103	11	-0.1959922360123656
1103	12	-0.1943305469788612
1103	13	-0.22533306015370158
1103	14	-0.24944207219150627
1103	15	-0.25408936611300703
1103	16	-0.26702783081163517
1103	17	-0.2541577278476197
1103	18	-0.31353212769354855
1103	19	-0.3099347138886941
1103	20	-0.30110610575983543
1103	21	-0.35545018959037833
1103	22	-0.40679619149681656
1103	23	-0.2952494792386773
1103	24	-0.3420922749535791
1103	25	-0.3560400537172228
1103	26	-0.386069918013348
1103	27	-0.28200127447281176
1103	28	-0.3088864307896344
1103	29	-0.3398635399541021
1103	30	-0.46197368183037923
1103	31	-0.4141117802250207
1103	32	-0.4395374480791432
1103	33	-0.3924375100422637
1103	34	-0.3266085649182173
1103	35	-0.38521803431343216
1103	36	-0.5838625088594185
1103	37	-0.44670457283630327
1103	38	-0.398500169948214
1103	39	-0.39411897445883426
1103	40	-0.451924707325027
1103	41	-0.45634280093982227
1103	42	-0.463024190455684
1103	43	-0.537937651426347
1103	44	-0.5137468283163216
1103	45	-0.5481339389519633
1103	46	-0.5519467440808228
1103	47	-0.5099227674541638
1103	48	-0.4852547081045344
1103	49	-0.47384506509863655
1103	50	-0.5091265607480366
1103	51	-0.6157292747890679
2204	1	0.600034491361626
2204	2	0.0262745957760373
2204	3	-0.016334428559829917
2204	4	-0.15123529962229298
2204	5	-0.19191304338760773
2204	6	-0.14697102790287886
2204	7	-0.17611503798546835
2204	8	-0.13854864190872007
2204	9	-0.21346149483937893
2204	10	-0.31333768352871516
2204	11	-0.3080053961142042
2204	12	-0.24666808104522175
2204	13	-0.2565385664413
2204	14	-0.2912446130617852
2204	15	-0.2850943441691598
2204	16	-0.27413654226370454
2204	17	-0.28474773545332255
2204	18	-0.2542473036201969
2204	19	-0.24816733578759198
2204	20	-0.23067984375710893
2204	21	-0.2793507348389639
2204	22	-0.3719779298347774
2204	23	-0.3480187684976457
2204	24	-0.34303990087861536
2204	25	-0.34186250223022085
2204	26	-0.3196060087545973
2204	27	-0.32631626011343684
2204	28	-0.37283782552800204
2204	29	-0.3588029187254165
2204	30	-0.4710448607934623
2204	31	-0.34571814582849214
2204	32	-0.35240528923239367
2204	33	-0.4243884899935608
2204	34	-0.4127203528670833
2204	35	-0.3919471495244409
2204	36	-0.42960958442226627
2204	37	-0.3766685309559179
2204	38	-0.3863831338376258
2204	39	-0.39556540023521336
2204	40	-0.37499133159769116
2204	41	-0.47134410843091246
2204	42	-0.7011431877391843
2204	43	-0.6142586695814884
2204	44	-0.4867221873815524
2204	45	-0.4177441593447071
2204	46	-0.33918916513242436
2204	47	-0.3796493508293679
2204	48	-0.46446339291511407
2204	49	-0.48475045453778165
2204	50	-0.47248521449503755
2204	51	-0.5483056004779883
2205	1	0.7581416584107927
2205	2	0.02101535851301861
2205	3	0.09450478174328936
2205	4	-0.10154400063470348
2205	5	-0.06319142051075488
2205	6	-0.11571822539352894
2205	7	-0.1792307183049502
2205	8	-0.16478265473356402
2205	9	-0.2902105922307143
2205	10	-0.2836889567800327
2205	11	-0.25474311165038444
2205	12	-0.23340827637631634
2205	13	-0.1892974780265959
2205	14	-0.16565363212134798
2205	15	-0.2865222595104413
2205	16	-0.3449857464921138
2205	17	-0.3202382708293783
2205	18	-0.3007380541593392
2205	19	-0.29407324135637936
2205	20	-0.42876867141252717
2205	21	-0.3656860070338155
2205	22	-0.34303487192224225
2205	23	-0.28490962261001584
2205	24	-0.24681138362348065
2205	25	-0.26311224908921105
2205	26	-0.2665202421321098
2205	27	-0.3773599214630252
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1210	15	0.28940710560973315
1210	16	0.29339766570054593
1210	17	0.3454509665540968
1210	18	0.3522233593916013
1210	19	0.3726515032072939
1210	20	0.32136021216805233
1210	21	0.3547907526944698
1210	22	0.3815012259811823
1210	23	0.39309152838422534
1210	24	0.4830466959100974
1210	25	0.39826762145637673
1210	26	0.31970287844050205
1210	27	0.3771293961872857
1210	28	0.45520956965517456
1210	29	0.4277909274200198
1210	30	0.5399533632100955
1210	31	0.5342536598936434
1210	32	0.501919763386752
1210	33	0.48659220609991394
1210	34	0.5017972364291126
1210	35	0.4628530303120897
1210	36	0.5498078798802126
1210	37	0.5901217456351517
1210	38	0.523050184454398
1210	39	0.5679139835082125
1210	40	0.560990569011814
1210	41	0.5578901195731092
1210	42	0.5441300648173879
1210	43	0.516270109670991
1210	44	0.566040664466243
1210	45	0.6549883617979049
1210	46	0.61079496361846
1210	47	0.6589232032396737
1210	48	0.6821298198231034
1210	49	0.5437335310997042
1210	50	0.6732036925236997
1210	51	0.8300104028928956
1211	1	-0.24212916582059307
1211	2	0.08147679586269874
1211	3	0.13246014421712005
1211	4	0.14180915177933429
1211	5	-2.0763590972035075
1211	6	-0.7451795557747616
1211	7	-0.279556771142218
1211	8	0.04734981022691187
1211	9	0.14120976435088295
1211	10	0.17020662216104654
1211	11	0.22653204353942868
1211	12	0.32660390747530954
1211	13	0.2438288757246312
1211	14	0.3332375487243695
1211	15	0.3438674268563844
1211	16	0.35176321809853306
1211	17	0.2585525951241152
1211	18	0.3342566435059666
1211	19	0.23760553659941763
1211	20	0.22148579663495838
1211	21	0.3550051561392209
1211	22	0.3718616154493688
1211	23	0.3989500611967358
1211	24	0.4237008541265155
1211	25	0.3933505585774526
1211	26	0.4068036057147211
1211	27	0.4619949229195086
1211	28	0.38832859687131105
1211	29	0.44697719845458295
1211	30	0.2858515703527189
1211	31	0.3607498670161249
1211	32	0.45309176216452585
1211	33	0.5005472356973328
1211	34	0.5753704058783242
1211	35	0.5641005262537888
1211	36	0.4814012683052482
1211	37	0.5416371702698228
1211	38	0.5271291384548675
1211	39	0.5809756385599769
1211	40	0.5706016560527161
1211	41	0.554406920487601
1211	42	0.6448348142704958
1211	43	0.643262707465631
1211	44	0.7144540040946197
1211	45	0.6315238206760441
1211	46	0.6744789678693124
1211	47	0.6721291381993666
1211	48	0.7198624266852178
1211	49	0.711422746192504
1211	50	0.7360130427414688
1211	51	0.7876234655364627
1208	1	-0.4068996997315537
1208	2	2.1098241639805337E-4
1208	3	0.10842988148564103
1208	4	0.1825647105903201
1208	5	0.25437290537821866
1208	6	0.17513341451559228
1208	7	0.1653501030447586
1208	8	0.031763288464468076
1208	9	0.08653799546424779
1208	10	0.05198788070973137
1208	11	0.1372020786447905
1208	12	0.19945690297926433
1208	13	0.2304935557560981
1208	14	0.26266609947624175
1208	15	0.17661622154182055
1208	16	0.18229287683017503
1208	17	0.20420129105293938
1208	18	0.27210609836093624
1208	19	0.2445923679867832
1208	20	0.2382606654555417
1208	21	0.3039481995770359
1208	22	0.36516697478514715
1208	23	0.2989155281856313
1208	24	0.31958523014082374
1208	25	0.3241721337620618
1208	26	0.2597496731858797
1208	27	0.3296897397236549
1208	28	0.30760112282231233
1208	29	0.35839626645182676
1208	30	0.37657234172056064
1208	31	0.3694811408124252
1208	32	0.3826311356400822
1208	33	0.38690563912926734
1208	34	-0.6888050679062019
1208	35	-0.19091078245384807
1208	36	0.055216190797168
1208	37	0.25562786257477654
1208	38	0.28078332257549476
1208	39	0.40950225533669027
1208	40	0.2894138721842978
1208	41	0.49501251126906
1208	42	0.4976332738409681
1208	43	0.5037338046976529
1208	44	0.5010524554609503
1208	45	0.5766202429276532
1208	46	0.4454671709460669
1208	47	0.51051353242989
1208	48	0.5344671922700854
1208	49	0.540017074888965
1208	50	0.530479373210639
1208	51	0.592571361656411
1209	1	-0.7616754223055509
1209	2	0.04241475795603833
1209	3	0.1490727221317769
1209	4	0.16188585742112593
1209	5	0.028026063962720116
1209	6	0.024294459688370296
1209	7	0.1246569922836045
1209	8	0.24105677236965306
1209	9	0.1930668858790483
1209	10	0.2845110427468853
1209	11	0.1952182435789993
1209	12	0.19601617936852733
1209	13	0.19093233363141593
1209	14	0.06890346014770898
1209	15	0.16526255837053583
1209	16	0.24898255536680836
1209	17	0.24449593354673027
1209	18	0.1942065064098344
1209	19	0.2584712140442065
1209	20	0.25304236354664766
1209	21	0.2827551348945434
1209	22	0.23171249392242288
1209	23	0.33854688621874374
1209	24	0.20145624859722489
1209	25	0.1913801692595456
1209	26	0.24580000460230167
1209	27	0.33058654669687115
1209	28	0.26527518482865986
1209	29	0.33264284663578536
1209	30	0.38790019576727985
1209	31	0.2945786063221689
1209	32	0.32984720924081046
1209	33	0.40377453573751154
1209	34	0.43638054182961383
1209	35	0.4183464673779369
1209	36	0.4167884490527882
1209	37	0.4019551714122258
1209	38	0.42557231523834105
1209	39	0.518768423668341
1209	40	0.5752212115508399
1209	41	0.5583814730246388
1209	42	0.5170374183588109
1209	43	0.5935736007111814
1209	44	0.5746115149306021
1209	45	0.7142218303359016
1209	46	0.6262530579150791
1209	47	0.6382534291801178
1209	48	0.5960578818971669
1209	49	0.6342428956967012
1209	50	0.7100220006165898
1209	51	0.7783498475934607
1214	1	-0.7944879578719295
1214	2	0.01350625540909789
1214	3	0.1375192565885257
1214	4	0.19103789288018902
1214	5	0.16291875446503212
1214	6	0.12218105811044921
1214	7	0.16262061048461618
1214	8	0.2475329964688484
1214	9	0.21542945364569732
1214	10	0.13781232515589892
1214	11	0.20848657854349995
1214	12	0.24217087935447523
1214	13	0.17490877420766537
1214	14	0.15423682229928914
1214	15	0.16302142829905364
1214	16	0.25088717859303955
1214	17	0.26309789778097326
1214	18	0.1581783559404144
1214	19	0.19635663426770122
1214	20	0.22669565072642683
1214	21	0.23998676257872376
1214	22	0.2539805088264515
1214	23	0.2545319416414671
1214	24	0.32430152021520087
1214	25	0.24574096477547158
1214	26	0.2052343721411205
1214	27	0.25652231279684656
1214	28	0.31007840489058225
1214	29	0.34124384257786033
1214	30	0.35715650327003345
1214	31	0.27565277154038625
1214	32	0.2991509560470291
1214	33	0.36498993400063284
1214	34	0.5143971625843236
1214	35	0.47889613736276004
1214	36	0.3705045622457561
1214	37	0.4391829955651474
1214	38	0.4778601804852869
1214	39	0.4656251264040421
1214	40	0.465547376999794
1214	41	0.43444410968789526
1214	42	0.4907243825497787
1214	43	0.4614587296106194
1214	44	0.4985728917428034
1214	45	0.5402977694241642
1214	46	0.4123136031371928
1214	47	0.523390513240777
1214	48	0.5734810309233893
1214	49	0.5785570047082444
1214	50	0.6433776594189595
1214	51	0.661478048172242
1215	1	-0.5102224876955361
1215	2	-0.04775340605348788
1215	3	0.041895735021917346
1215	4	0.1231492505000844
1215	5	0.07265769408833478
1215	6	0.09410181806799756
1215	7	-0.08756095144568832
1215	8	-0.01772264885100583
1215	9	-0.0641807300799968
1215	10	0.10923731925436897
1215	11	-0.020564004180073425
1215	12	0.1122788233878893
1215	13	0.06349127078835437
1215	14	0.12568128121161237
1215	15	0.09340931040742362
1215	16	0.2010767216990459
1215	17	0.17141023825814727
1215	18	0.0271822363758929
1215	19	0.17508084934627277
1215	20	0.09593275948881086
1215	21	0.0030145394840701556
1215	22	0.0496341044329327
1215	23	-0.003343298846829157
1215	24	-6.658955443228365E-4
1215	25	0.1798506746146984
1215	26	0.05042008034131129
1215	27	0.11744641655548094
1215	28	-0.014750827715836579
1215	29	0.09301559684179495
1215	30	0.21747180752197437
1215	31	0.17626872227443613
1215	32	0.15797644796464283
1215	33	0.30682917666327825
1215	34	0.20052441354987138
1215	35	0.29143120124638955
1215	36	0.3214189207186777
1215	37	0.23326481319991643
1215	38	0.3071364269958039
1215	39	0.12729279026278562
1215	40	0.1973193408784013
1215	41	0.41040127030474594
1215	42	0.3360754135798132
1215	43	0.446972511629788
1215	44	0.46715722975341833
1215	45	0.41220355633461025
1215	46	0.6184291043114101
1215	47	0.4614471292513045
1215	48	0.4735460891070389
1215	49	0.4843716419535866
1215	50	0.5876490487021258
1215	51	0.7038887711337125
1212	1	0.45831430089469904
1212	2	-0.12678293605309676
1212	3	0.008959454120173405
1212	4	0.0659010500970254
1212	5	0.06845622242456528
1212	6	-0.3009408510344116
1212	7	-0.21976250090897764
1212	8	-0.17672714984554716
1212	9	-0.07816917039342997
1212	10	-0.21545238510851306
1212	11	-0.12366508310037716
1212	12	-0.0561688388979249
1212	13	-0.17511511307954208
1212	14	-0.30071990955951833
1212	15	-0.3363522235873546
1212	16	-0.45530195879471336
1212	17	-0.5193989858462089
1212	18	-0.3398036373492559
1212	19	-0.588489517406579
1212	20	-0.18747560707242883
1212	21	-0.22671016930011234
1212	22	-0.21121345093119004
1212	23	-0.24262804386648185
1212	24	-0.4639141872056456
1212	25	-0.38825769223468143
1212	26	-0.3169461136709586
1212	27	-0.343051923841287
1212	28	-0.0886630666567143
1212	29	-0.07123117414109004
1212	30	0.014438501103640533
1212	31	0.09651237549822866
1212	32	-0.0830226033459951
1212	33	-0.11642361182041583
1212	34	-0.326173572094028
1212	35	-0.4866496238370104
1212	36	-0.17914768148660443
1212	37	-0.5239375081624758
1212	38	-0.32034178618768294
1212	39	-0.16708227893076355
1212	40	-0.0642803729145669
1212	41	-0.09468304981034237
1212	42	0.058526436755840905
1212	43	0.049125563168026076
1212	44	0.05075541342916523
1212	45	0.11406070807821322
1212	46	0.1232111581062938
1212	47	0.19187308626992206
1212	48	-0.05892359333677177
1212	49	-0.24610592687380262
1212	50	-0.3624186415891728
1212	51	-0.42720586751647716
1213	1	-0.09829623607090099
1213	2	-0.044931112593658895
1213	3	-0.008012322802606775
1213	4	-0.026548086277919936
1213	5	-0.007016177829704873
1213	6	-0.02015037383212359
1213	7	-0.025545415158802598
1213	8	-0.0408298753526708
1213	9	-0.03444161502018517
1213	10	-0.24569740643716642
1213	11	-0.23034790775361103
1213	12	-0.3421517533338019
1213	13	-0.2296240120328008
1213	14	-0.14250484061295765
1213	15	-0.21646982495281009
1213	16	-0.21971718315279531
1213	17	-0.244882509176243
1213	18	-0.11706747589329325
1213	19	-0.17059901870501193
1213	20	-0.1457050142479659
1213	21	-0.09485644060373488
1213	22	-0.1331957037394389
1213	23	-0.32375981635531303
1213	24	-0.13744718264698008
1213	25	-0.04715730100141968
1213	26	-0.004260857241803251
1213	27	-0.15596274058162862
1213	28	-0.04372450124881766
1213	29	0.04985130895563117
1213	30	-0.16147263311304982
1213	31	0.10137257928567323
1213	32	-0.10767871918368854
1213	33	-0.057186842367002555
1213	34	-0.023626769873935416
1213	35	-0.08246904243640785
1213	36	0.13425537417089828
1213	37	-0.011344528376682206
1213	38	0.011969315594413388
1213	39	0.08594833402950286
1213	40	0.07152477079011987
1213	41	0.022959101994487696
1213	42	0.026608991073821642
1213	43	0.20580035425368237
1213	44	0.2361406447053369
1213	45	0.12750492895835208
1213	46	0.09416716627376331
1213	47	0.17541153577489865
1213	48	0.07396688427628106
1213	49	0.25644416053992813
1213	50	0.1446958929728055
1213	51	-0.10519433717056614
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	61.0
14	193.0
15	487.0
16	1010.0
17	1970.0
18	3678.0
19	8662.0
20	19934.0
21	47867.0
22	107330.0
23	215277.0
24	387847.0
25	633441.0
26	949199.0
27	1323183.0
28	1745056.0
29	2199969.0
30	2676410.0
31	3177083.0
32	3721210.0
33	4331544.0
34	5081245.0
35	6101910.0
36	7665444.0
37	1.0378188E7
38	1.5651421E7
39	1.5989746E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.579693384315497	28.947134905247722	17.59557528373423	25.877596426702553
2	13.681578258783622	23.174162234994807	40.90013746063207	22.244122045589503
3	16.345437726590422	28.64656833839244	32.535836635176935	22.472157299840198
4	12.829666191081273	21.826108133539098	40.93068298417328	24.41354269120634
5	12.344596862003735	38.88777884745652	36.27489473163254	12.4927295589072
6	23.65194557773111	43.29213825941607	20.100855587196786	12.955060575656038
7	21.277145243717904	34.86944482040113	25.98630309757907	17.867106838301897
8	20.307827895691407	40.59486213470752	22.32490723114759	16.772402738453486
9	21.095391585516442	18.705801498157605	28.499134535929333	31.699672380396613
10	15.958431420110658	30.831044004876233	33.08737050949894	20.123154065514164
11	30.457856227624948	27.65796815277613	25.419023485329895	16.465152134269026
12	18.414357858564454	28.269753979908074	31.977951638210854	21.33793652331662
13	24.91497811327498	24.914753651465844	27.815508796803893	22.354759438455275
14	19.243305026151255	24.838365051242935	28.711778734138772	27.206551188467042
15	21.488307735829952	32.55498713736769	24.899689230805294	21.057015895997065
16	20.403879350394423	32.821292267644964	26.76410905660206	20.010719325358554
17	18.93981567390363	31.7732764408792	29.939777745924424	19.347130139292748
18	19.27909637159791	31.133459580361244	29.692158757930752	19.895285290110092
19	19.64322195829558	31.11380643233776	30.21348647598187	19.029485133384792
20	19.671392521995376	30.178060335965213	30.158219125344885	19.992328016694522
21	19.73442382461423	30.153234859875045	29.869143861164865	20.24319745434586
22	20.189896874479718	30.065046848334166	30.049049394531863	19.696006882654256
23	19.52710968439141	30.75951833941552	29.373352617150683	20.340019359042387
24	20.37564448141957	30.292795506337672	29.29825618896413	20.033303823278622
25	20.015991144605504	30.645080429276778	29.168576715331685	20.17035171078603
26	19.903012825228387	30.80496350911572	29.06778773348992	20.22423593216598
27	19.773703625314727	30.907485211297647	29.185560571497376	20.13325059189025
28	19.313555467066717	30.725993333359945	29.466016635672588	20.49443456390075
29	19.289568896885793	30.26872714735746	29.708448950796356	20.733255004960384
30	19.968907654403747	30.13831247669002	30.024407998473507	19.86837187043272
31	19.443373276008145	30.557423300399883	29.657982365994524	20.34122105759745
32	19.961415773578892	30.16824588445758	29.890937531275423	19.979400810688105
33	19.753738746242274	30.377126351557564	29.67248390711675	20.19665099508341
34	19.36637304839711	30.125012399676383	29.824804024442	20.683810527484507
35	19.875891702058738	29.714590544859114	30.0852219971311	20.324295755951045
36	19.763878973033666	30.229307773712044	30.07664843173956	19.93016482151473
37	19.507772980451385	30.239111402966007	30.060386952492635	20.192728664089973
38	19.38588377777797	29.504158608204435	30.64598361194426	20.463974002073336
39	19.532126135825596	29.559678742342754	30.612848401181076	20.295346720650574
40	18.707231267107826	29.247236083320626	31.600602533799698	20.444930115771847
41	19.06418421242145	29.346009356374186	31.267840331907454	20.321966099296915
42	19.378642710124154	28.70563691547187	31.537848511406448	20.377871862997527
43	18.793239289383674	28.78306352239621	31.731248748899755	20.69244843932036
44	18.21008106320587	28.973690725676366	32.23748081603786	20.57874739507991
45	18.466679919040878	28.365436609248164	32.42329005393299	20.744593417777967
46	18.152473605763024	28.781938544372665	32.546455091081256	20.51913275878305
47	17.586801629393598	28.258793867353454	32.87732374826923	21.27708075498372
48	17.77544796798347	28.070548625109552	32.74459615508986	21.409407251817118
49	17.539657094577564	28.334664175977338	33.25201662125373	20.873662108191375
50	16.76500957778501	28.820749106296457	33.156776081322775	21.257465234595763
51	16.971040352624396	28.42288234140735	33.244130125856856	21.361947180111393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	279.0
1	473.0
2	667.0
3	1117.5
4	1568.0
5	4358.5
6	7149.0
7	9929.0
8	12709.0
9	22362.0
10	32015.0
11	58345.5
12	84676.0
13	125635.0
14	166594.0
15	229870.5
16	293147.0
17	424847.0
18	556547.0
19	709403.0
20	862259.0
21	1062852.0
22	1263445.0
23	1523219.5
24	1782994.0
25	2119805.5
26	2810761.0
27	3164905.0
28	3572278.5
29	3979652.0
30	4318548.5
31	4657445.0
32	4986209.0
33	5314973.0
34	5549702.0
35	5784431.0
36	5975455.0
37	6166479.0
38	6268800.5
39	6371122.0
40	6312559.5
41	6253997.0
42	6073220.0
43	5892443.0
44	5621424.5
45	5350406.0
46	4984598.5
47	4618791.0
48	4215702.0
49	3812613.0
50	3558923.5
51	3305234.0
52	3025931.0
53	2746628.0
54	2404530.0
55	2062432.0
56	1928262.0
57	1794092.0
58	1442784.5
59	1091477.0
60	936191.5
61	780906.0
62	665074.0
63	549242.0
64	466743.0
65	384244.0
66	329652.5
67	275061.0
68	236816.5
69	198572.0
70	163752.0
71	128932.0
72	103585.5
73	78239.0
74	64460.5
75	45853.0
76	41024.0
77	33042.0
78	25060.0
79	17885.0
80	10710.0
81	7998.5
82	5287.0
83	3791.5
84	2296.0
85	1507.5
86	719.0
87	510.5
88	302.0
89	208.5
90	115.0
91	100.5
92	86.0
93	58.5
94	31.0
95	21.0
96	11.0
97	24.5
98	38.0
99	30.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07609619321750181
2	1.601565340844772E-4
3	8.493149534782882E-6
4	2.8876708418261796E-4
5	2.426614152795109E-5
6	6.442660575671015E-4
7	1.2133070763975544E-6
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.8199606145963317E-5
27	4.489236182670951E-4
28	0.0011368687305845086
29	0.0016755770725050225
30	0.00356712280460881
31	0.0050607038156542
32	0.007782151588013915
33	0.009441955668525768
34	0.009634871493672979
35	0.008869274728466122
36	0.007837963713528202
37	0.008525908825845615
38	0.011043521009370541
39	0.011377180455379868
40	0.007357494111274771
41	0.007317454977753652
42	0.007447278834928189
43	0.008377885362525112
44	0.005059490508577802
45	0.004786496416388352
46	0.005919725225743668
47	0.004756163739478413
48	0.005382230190899551
49	0.005050997359043019
50	0.005063130429806995
51	0.00233682942914169
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8.2419366E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.020636967882737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.26028446163033	15.364446340614535
2	5.294869612967683	1.9083384617512875
3	0.7515668704027954	0.40631141185849645
4	0.5295390842226666	0.38170526388327025
5	0.4919831042861239	0.4432924458336117
6	0.3188936321776966	0.34479998261062794
7	0.30263844288039066	0.38176162581709844
8	0.2135658630472668	0.30788743093658866
9	0.13668887910459446	0.2216898601101638
>10	3.5734102759203363	16.965755810040783
>50	1.7839237109514678	23.20487249224568
>100	1.322916167120328	35.357704835863466
>500	0.013430483328878907	1.6308406553571082
>1k	0.005975947487497765	1.8452262061696743
>5k	2.1030047322134188E-4	0.2545455583008121
>10k+	1.0316399874330108E-4	0.9808216186066545
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	203422	0.24681335209494332	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	155477	0.18864134431706256	No Hit
CTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG	146405	0.17763422251998395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.2133070763975544E-6	0.0	0.5708925254290357
2	1.2133070763975544E-6	0.0	0.5759204699536271
3	1.2133070763975544E-6	0.0	0.5782900586738315
4	1.2133070763975544E-6	0.0	0.8027542944215319
5	1.2133070763975544E-6	0.0	0.8269609839027395
6	3.6399212291926635E-6	0.0	0.83013014198629
7	6.066535381987772E-6	0.0	0.8336232530592386
8	6.066535381987772E-6	0.0	0.8367972643710946
9	6.066535381987772E-6	0.0	0.8542361269801566
10	1.4195692793851386E-4	0.0	0.879653696923609
11	1.4195692793851386E-4	0.0	0.8915720123350621
12	1.4438354209130898E-4	0.0	0.9087148280174832
13	1.4438354209130898E-4	0.0	0.915355257646607
14	1.4559684916770654E-4	0.0	0.9652051921874769
15	1.492367703968992E-4	0.0	0.9671137242186503
16	1.492367703968992E-4	0.0	0.9678344286220304
17	1.5045007747329674E-4	0.0	0.9681680880680398
18	1.5287669162609185E-4	0.0	0.9683585772790342
19	1.5651661285528452E-4	0.0	0.9690186163285944
20	1.5772991993168208E-4	0.0	0.9699334498641982
21	1.5772991993168208E-4	0.0	0.970326561356951
22	1.5772991993168208E-4	0.0	0.9713311796162082
23	1.601565340844772E-4	0.0	0.9718213556750728
24	1.6136984116087475E-4	0.0	0.9779667560170264
25	1.6258314823727228E-4	0.0	0.9809709043381867
26	1.6258314823727228E-4	0.0	0.9827144266069701
27	1.6258314823727228E-4	0.0	0.9940831139128151
28	1.6379645531366984E-4	0.0	1.0370378243385177
29	1.7228960484845274E-4	0.0	1.0932697541012388
30	1.735029119248503E-4	0.0	1.1551957339734935
31	2.899803912590155E-4	0.0	1.231907073878729
32	2.9119369833541307E-4	0.0	1.347663848809514
33	2.924070054118106E-4	0.0	1.495273574416964
34	2.9726023371740083E-4	0.0	1.694060592506863
35	3.033267690993886E-4	0.0	1.9805660722990759
36	3.033267690993886E-4	0.0	2.3995476985348323
37	3.045400761757862E-4	1.2133070763975544E-6	2.9799586179782067
38	3.057533832521837E-4	2.426614152795109E-6	3.6695744541398194
39	3.227396823217495E-4	2.426614152795109E-6	4.431112707176126
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	34480	0.0	28.862494	45
ATGCCGT	38370	0.0	25.842615	44
TATGCCG	41530	0.0	24.223383	43
GTATGCC	44905	0.0	22.543262	42
CGTATGC	45820	0.0	22.19625	41
TCGTATG	50835	0.0	19.95799	40
CTCGTAT	76060	0.0	16.734549	45
TCTCGTA	67050	0.0	16.09406	44
ATCTCGT	70775	0.0	15.164586	43
TTATACC	56865	0.0	13.693255	8
GCTGATC	75090	0.0	13.391623	39
CCGAGGC	96220	0.0	13.362436	34
ACCGAGG	97280	0.0	13.323258	33
CGAGGCT	96535	0.0	13.297854	35
GATCTCG	76250	0.0	13.187606	42
TATACCT	59600	0.0	13.083756	9
GGCTGAT	83870	0.0	13.017428	38
TGATCTC	77375	0.0	12.946452	41
AGGCTGA	99050	0.0	12.926154	37
CTGATCT	79785	0.0	12.662648	40
>>END_MODULE