FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n01 z_rfp_26_feb.34100000003071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n01 z_rfp_26_feb.34100000003071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82419366
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT2034220.24681335209494332No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC1554770.18864134431706256No Hit
CTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG1464050.17763422251998395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC344800.028.86249445
ATGCCGT383700.025.84261544
TATGCCG415300.024.22338343
GTATGCC449050.022.54326242
CGTATGC458200.022.1962541
TCGTATG508350.019.9579940
CTCGTAT760600.016.73454945
TCTCGTA670500.016.0940644
ATCTCGT707750.015.16458643
TTATACC568650.013.6932558
GCTGATC750900.013.39162339
CCGAGGC962200.013.36243634
ACCGAGG972800.013.32325833
CGAGGCT965350.013.29785435
GATCTCG762500.013.18760642
TATACCT596000.013.0837569
GGCTGAT838700.013.01742838
TGATCTC773750.012.94645241
AGGCTGA990500.012.92615437
CTGATCT797850.012.66264840
Produced by FastQC (version 0.11.2)