##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H3NH5BCXX l02n01 gdna_2000_dn_3_march.34100000003120.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31283147 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.94044624091048 38.0 32.0 38.0 27.0 38.0 2 34.719883009212595 38.0 32.0 38.0 32.0 38.0 3 34.74355978316376 38.0 32.0 38.0 32.0 38.0 4 35.03556633864234 38.0 32.0 38.0 32.0 38.0 5 35.0543535789414 38.0 32.0 38.0 32.0 38.0 6 36.854667243036644 38.0 38.0 40.0 32.0 40.0 7 36.660549656337324 38.0 38.0 40.0 32.0 40.0 8 36.58301864579033 38.0 38.0 40.0 32.0 40.0 9 36.564712431265306 38.0 38.0 40.0 32.0 40.0 10 36.49710027574911 38.0 38.0 40.0 32.0 40.0 11 36.536215681881366 38.0 38.0 40.0 32.0 40.0 12 36.48067616087346 38.0 38.0 40.0 32.0 40.0 13 36.462345108693825 38.0 38.0 40.0 32.0 40.0 14 36.41625997537908 38.0 38.0 40.0 32.0 40.0 15 36.41797847256224 38.0 38.0 40.0 32.0 40.0 16 36.42559650408573 38.0 38.0 40.0 32.0 40.0 17 36.41263300651945 38.0 38.0 40.0 32.0 40.0 18 36.39310373729344 38.0 38.0 40.0 32.0 40.0 19 36.36809675829609 38.0 38.0 40.0 32.0 40.0 20 36.36330878092284 38.0 38.0 40.0 32.0 40.0 21 36.33473668745667 38.0 38.0 40.0 32.0 40.0 22 36.31714542657745 38.0 38.0 40.0 32.0 40.0 23 36.29191235140122 38.0 38.0 40.0 32.0 40.0 24 36.270350805818865 38.0 38.0 40.0 32.0 40.0 25 36.2409711529342 38.0 38.0 40.0 32.0 40.0 26 36.101987501449265 38.0 38.0 40.0 32.0 40.0 27 36.02798497222802 38.0 38.0 40.0 27.0 40.0 28 35.98935487532632 38.0 38.0 40.0 27.0 40.0 29 35.96904761531824 38.0 38.0 40.0 27.0 40.0 30 35.91877220664532 38.0 38.0 40.0 27.0 40.0 31 35.89810836486495 38.0 38.0 40.0 27.0 40.0 32 35.88339200656507 38.0 38.0 40.0 27.0 40.0 33 35.85428483266086 38.0 38.0 40.0 27.0 40.0 34 35.79233131500485 38.0 38.0 40.0 27.0 40.0 35 35.76555622744732 38.0 38.0 40.0 27.0 40.0 36 35.74264261201087 38.0 38.0 40.0 27.0 40.0 37 35.72028801961644 38.0 38.0 40.0 27.0 40.0 38 35.712996202076475 38.0 38.0 40.0 27.0 40.0 39 35.6865807970023 38.0 38.0 40.0 27.0 40.0 40 35.6502451943214 38.0 38.0 40.0 27.0 40.0 41 35.62944981206654 38.0 38.0 40.0 27.0 40.0 42 35.58786051799712 38.0 38.0 40.0 27.0 40.0 43 35.566557514178484 38.0 38.0 40.0 27.0 40.0 44 35.53221528511821 38.0 38.0 40.0 27.0 40.0 45 35.49985089415716 38.0 38.0 40.0 27.0 40.0 46 35.480972678356174 38.0 38.0 40.0 27.0 40.0 47 35.45312982737958 38.0 38.0 40.0 27.0 40.0 48 35.42633853301268 38.0 38.0 40.0 27.0 40.0 49 35.41994966171402 38.0 38.0 40.0 27.0 40.0 50 35.39495482983218 38.0 38.0 40.0 27.0 40.0 51 34.790971381491765 38.0 32.0 38.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.9507023381059838 2201 2 0.24357928090451253 2201 3 0.2519249177249989 2201 4 -0.05855089334207264 2201 5 -0.0066725462675094604 2201 6 0.17622635572544 2201 7 0.13993526547723434 2201 8 0.08783877043747879 2201 9 0.06801178609914871 2201 10 0.14856664064927827 2201 11 0.07700073242598648 2201 12 0.09983138184214368 2201 13 0.16957065676883332 2201 14 -0.08308066979795825 2201 15 -0.0503491274722947 2201 16 -0.10457296057231247 2201 17 -0.14436109788408658 2201 18 -0.17141686481178908 2201 19 -0.11766696867053383 2201 20 -0.08527606342182281 2201 21 0.05654424137112102 2201 22 0.06345778528642398 2201 23 -0.0968513182404962 2201 24 -0.0332155300365784 2201 25 -0.06993117624109857 2201 26 0.0760407828032541 2201 27 -0.15939829581969178 2201 28 -0.17019566328283986 2201 29 -0.1550717784141895 2201 30 -0.02914605936685888 2201 31 0.013337308967209083 2201 32 -0.10074182430437162 2201 33 -0.035796860361585914 2201 34 -0.13007621716958795 2201 35 0.010019961963870117 2201 36 0.04016107592038054 2201 37 0.03920531358254209 2201 38 -0.06572566077553432 2201 39 -0.16047557262906054 2201 40 -0.20851161771072668 2201 41 -0.09928585877470653 2201 42 -0.23418323502850313 2201 43 -0.2658138138836321 2201 44 -0.09622153111916276 2201 45 -0.05461706579035308 2201 46 -0.2811692642646193 2201 47 -0.1993702270777007 2201 48 -0.28285543669731794 2201 49 -0.0755222482634963 2201 50 -0.18175737276430226 2201 51 -0.13669940371266165 1101 1 0.8774801468335554 1101 2 0.23373493294534597 1101 3 0.14542265113886543 1101 4 0.021731507899438895 1101 5 0.123658432182161 1101 6 0.11536551279029794 1101 7 0.19181963471497454 1101 8 0.13489827851007874 1101 9 0.13524245152498793 1101 10 0.09611955198966626 1101 11 0.08492653035997932 1101 12 0.04772870100718052 1101 13 0.027297236470943176 1101 14 0.047545038547852414 1101 15 0.049880453230144894 1101 16 0.023733301713562582 1101 17 0.008745814747641134 1101 18 0.101277144536283 1101 19 0.052414549901577345 1101 20 -0.03781089982771846 1101 21 0.06081453810393356 1101 22 0.031354560743587 1101 23 0.039647482338878604 1101 24 0.025587500325023882 1101 25 0.005911645920825492 1101 26 -0.03717403499420868 1101 27 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46 -0.15639712410229833 2202 47 -0.20344940711628823 2202 48 -0.2011551881994933 2202 49 -0.2248452268959511 2202 50 -0.17004357340961462 2202 51 -0.22731337634451876 1102 1 0.7204188262277569 1102 2 -0.013789888853473542 1102 3 0.004434244239988061 1102 4 -0.16029588564201447 1102 5 -0.07822685751072811 1102 6 -0.056685443522155765 1102 7 -0.08411844314840522 1102 8 0.04698269676831757 1102 9 -0.03376225343198769 1102 10 -0.028029025279359132 1102 11 -0.15110634094433095 1102 12 -0.11831875273462344 1102 13 -0.1890187595048971 1102 14 -0.13255890363157619 1102 15 -0.1055193398047578 1102 16 -0.13497523457100158 1102 17 -0.12184806187793384 1102 18 -0.13910975011107496 1102 19 -0.19083022224182145 1102 20 -0.15159788793773998 1102 21 -0.08682982043004728 1102 22 -0.16030854113966342 1102 23 -0.16903001129169581 1102 24 -0.20799510743179894 1102 25 -0.22620160884376617 1102 26 -0.1838820043574998 1102 27 -0.2508446930324695 1102 28 -0.2756371373296247 1102 29 -0.26187383346409376 1102 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-0.3462075770158677 1213 31 -0.06470388112768433 1213 32 -0.24406259876943182 1213 33 -0.24986395774156733 1213 34 -0.1615561672573591 1213 35 -0.1771002272545701 1213 36 -0.059883049006089095 1213 37 -0.2053306770246195 1213 38 -0.17930023251094696 1213 39 -0.12949859369931005 1213 40 -0.1136538470210482 1213 41 -0.22470791139940616 1213 42 -0.20293703271955366 1213 43 -0.012169085823359183 1213 44 0.05379231981616073 1213 45 -0.06640416022822393 1213 46 -0.0768847459180435 1213 47 -0.05554187804957422 1213 48 -0.052230315214508494 1213 49 0.10512641195778372 1213 50 -0.07991219519734472 1213 51 -0.37395308470328104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 13.0 14 66.0 15 138.0 16 363.0 17 574.0 18 1186.0 19 2890.0 20 7034.0 21 17030.0 22 37969.0 23 76859.0 24 139589.0 25 228586.0 26 345886.0 27 484412.0 28 643405.0 29 815891.0 30 998720.0 31 1190294.0 32 1400123.0 33 1636927.0 34 1925668.0 35 2319984.0 36 2920593.0 37 3957887.0 38 5972137.0 39 6158923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.642488962094944 22.97264969415948 10.30711950714387 30.077741836601703 2 16.287916953509473 23.383044086673753 35.13552555398595 25.19351340583083 3 18.99144031102243 26.722827045591895 31.122029734477476 23.163702908908203 4 13.98125873635875 18.238601752974517 40.1008360564262 27.679303454240532 5 13.34586574922256 38.16663742301324 35.80541672589719 12.682080101867008 6 26.95944815462831 42.338552552849364 17.631566648763418 13.070432643758906 7 22.872423928449397 33.7035209405243 25.060119431078974 18.363935699947323 8 22.75292508135451 36.26350635375655 23.1061248409567 17.877443723932252 9 22.0060341115937 15.158308721306076 27.80321941395474 35.03243775314549 10 15.15804340273055 25.6279523284534 36.371708383430864 22.842295885385187 11 32.745385238895565 28.168141140020214 24.399281184850103 14.687192436234117 12 17.366622354202406 27.689384958616852 34.224513921185746 20.719478765994996 13 25.798555369125747 24.876845030968273 27.11110873851662 22.213490861389364 14 18.56613402737263 24.451561730666036 28.88489128027944 28.097412961681894 15 22.142737110176288 32.87383459215277 24.570085611911104 20.413342685759844 16 20.214865211610583 32.461408054630816 27.2079947711143 20.115731962644297 17 19.42149234538328 31.275712127044 29.464196808588344 19.83859871898438 18 19.339016627706926 31.135898827570003 30.193170782977813 19.331913761745263 19 19.61807742680108 30.997322615912008 30.29132267287559 19.093277284411318 20 19.752421327688037 30.843399482795004 29.842959853111967 19.561219336404996 21 20.101679667969467 30.209096290728038 29.586406380406675 20.102817660895816 22 20.327152508026128 29.81643758538743 29.420588663921826 20.43582124266462 23 20.28705424041897 29.85009468516707 29.099904814563573 20.76294625985039 24 20.426017241807546 29.65537642360598 29.361959651949338 20.55664668263714 25 19.978066784649258 29.89878543869004 29.475039707482114 20.64810806917859 26 19.743865897877782 30.157087152109774 29.907182602873934 20.191864347138512 27 19.589083659149168 30.322318728280944 29.915004981937482 20.173592630632413 28 19.57313860737726 30.431437389509973 29.838047430599325 20.15737657251344 29 19.556123381719143 30.143046992459755 30.0220822035673 20.278747422253808 30 19.866519140282453 30.1725319614215 30.12226677451966 19.83868212377639 31 19.61705991802722 29.61215160194471 29.932528395922407 20.838260084105663 32 19.45067244862448 30.188568983982933 29.832129366191108 20.52862920120148 33 19.51109905096003 30.029921124011764 29.80991323532599 20.649066589702215 34 19.413980014703235 29.863208018261776 29.964444596191147 20.75836737084384 35 19.520251433907855 29.48940243047194 30.169776774466666 20.82056936115354 36 19.031405669805967 29.569965914823086 30.63519282927748 20.76343558609347 37 19.103101706078846 29.11550909304402 31.046823683681612 20.73456551719552 38 18.85894228694283 28.80214515780977 31.621234185055826 20.717678370191578 39 18.85782832354118 28.561213346406372 31.665699104411075 20.91525922564137 40 18.78960761824287 27.96503324679068 32.229441889070124 21.015917245896333 41 18.55422539493226 27.845738237867195 32.02795990682375 21.572076460376792 42 18.373023096358406 27.418310914833484 32.27968909552428 21.92897689328382 43 18.073440146160998 26.888111871345078 32.6209892241431 22.41745875835083 44 17.40013242279237 26.793755976352124 33.01646359536832 22.789648005487184 45 17.277971813811675 26.450803319409964 33.892616591474685 22.378608275303673 46 17.02347442394136 26.44935176100377 34.25037348115638 22.276800333898482 47 16.844991602108074 26.52210592404623 34.241466085879075 22.391436387966625 48 16.359726302459936 26.68916726536911 34.18748784027179 22.763618591899156 49 15.844647639273088 26.89407932500717 33.97068810595456 23.29058492976518 50 15.322991609805026 27.23359423083827 33.76754340158649 23.675870757770223 51 14.906175489357546 27.72575569862764 33.62835077719631 23.73971803481851 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 74.0 1 149.5 2 225.0 3 578.5 4 932.0 5 6755.0 6 12578.0 7 14719.5 8 16861.0 9 23374.0 10 29887.0 11 38444.0 12 47001.0 13 53805.5 14 60610.0 15 82833.0 16 105056.0 17 164946.5 18 224837.0 19 296936.5 20 369036.0 21 455240.0 22 541444.0 23 599559.5 24 657675.0 25 762544.0 26 974789.0 27 1082165.0 28 1210829.5 29 1339494.0 30 1457464.0 31 1575434.0 32 1695555.0 33 1815676.0 34 1917354.5 35 2019033.0 36 2096662.0 37 2174291.0 38 2211362.5 39 2248434.0 40 2246655.0 41 2244876.0 42 2208888.0 43 2172900.0 44 2106209.5 45 2039519.0 46 1938537.0 47 1837555.0 48 1702095.0 49 1566635.0 50 1427714.0 51 1288793.0 52 1182430.0 53 1076067.0 54 973092.5 55 870118.0 56 777643.0 57 685168.0 58 614510.0 59 543852.0 60 490065.5 61 436279.0 62 383170.0 63 330061.0 64 289474.5 65 248888.0 66 227603.5 67 206319.0 68 187117.5 69 167916.0 70 146288.0 71 124660.0 72 103666.0 73 82672.0 74 71174.0 75 58862.5 76 58049.0 77 45602.0 78 33155.0 79 23037.0 80 12919.0 81 9450.0 82 5981.0 83 3959.0 84 1937.0 85 1254.0 86 571.0 87 414.5 88 258.0 89 179.5 90 101.0 91 76.0 92 51.0 93 30.0 94 9.0 95 7.0 96 5.0 97 2.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07686247166885096 2 1.374542017783569E-4 3 3.1966093436827184E-6 4 2.908914502751274E-4 5 1.9179656062096312E-5 6 5.785862912065721E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 9.589828031048156E-6 27 4.379354800845324E-4 28 0.0011060268329142206 29 0.001566338578404532 30 0.003385209294959999 31 0.004932368217302434 32 0.007649486159432745 33 0.009286150143398297 34 0.009433194173207702 35 0.008694777414816994 36 0.007652682768776428 37 0.008272824981450875 38 0.010858881940490195 39 0.011207312358951612 40 0.00713802866444351 41 0.007074096477569855 42 0.007304252350315011 43 0.008221679231951952 44 0.00486843603042878 45 0.004612707282934163 46 0.005699554459786287 47 0.004753358094056202 48 0.005143344433985494 49 0.004830076718304588 50 0.004839666546335636 51 0.0022472163686089513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3.1283147E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.69746974688859 #Duplication Level Percentage of deduplicated Percentage of total 1 91.23809319389606 70.8896898569654 2 6.4984321802256595 10.098234754505809 3 1.092537745138781 2.546622552007629 4 0.3677794677546098 1.1430213627756232 5 0.18551260807215864 0.7206930126676472 6 0.13328767467142424 0.6213669042249668 7 0.05027495277854824 0.27343656357762525 8 0.04999572182375601 0.31076328671001113 9 0.040890642662229595 0.28593895240614736 >10 0.2700828669477223 4.148352487481034 >50 0.04114542149992547 2.180526192690731 >100 0.02887465816910496 4.418275226282044 >500 0.0024228064783018374 1.2460907796068195 >1k 6.368277850038617E-4 0.8440246343476654 >5k 2.912293556566831E-5 0.17074230229243614 >10k+ 4.109161133616291E-6 0.10222113145844441 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTTTGGGAAACATGAAAAAATTGATTTTTTTTTGTTTTTTTGCATAAAAA 32017 0.10234584135668959 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.023763593860937328 2 0.0 6.393218687365437E-6 0.02383711587584203 3 0.0 6.393218687365437E-6 0.023872278578622542 4 0.0 6.393218687365437E-6 0.029853134660652907 5 0.0 6.393218687365437E-6 0.031387507145620615 6 0.0 1.598304671841359E-5 0.03151537151936792 7 3.1966093436827184E-6 1.598304671841359E-5 0.03175192061080044 8 3.1966093436827184E-6 1.598304671841359E-5 0.03182224601636146 9 6.393218687365437E-6 1.598304671841359E-5 0.032451978057066955 10 9.909488965416427E-5 2.237626540577903E-5 0.03261500513359478 11 9.909488965416427E-5 2.237626540577903E-5 0.03273008306996735 12 9.909488965416427E-5 2.237626540577903E-5 0.032966632161399875 13 1.0548810834152971E-4 2.237626540577903E-5 0.033052940613679305 14 1.0868471768521243E-4 2.237626540577903E-5 0.033238343955612905 15 1.1188132702889514E-4 3.196609343682718E-5 0.033270310049049734 16 1.1188132702889514E-4 3.51627027805099E-5 0.03330227614248656 17 1.1507793637257786E-4 4.1555921467875335E-5 0.03352284218720067 18 1.374542017783569E-4 4.475253081155806E-5 0.03403749629153358 19 1.406508111220396E-4 4.794914015524078E-5 0.034385926709995004 20 1.406508111220396E-4 5.1145749498923495E-5 0.03545359423078503 21 1.5024063915308777E-4 5.1145749498923495E-5 0.035875546664151145 22 1.5343724849677047E-4 5.1145749498923495E-5 0.036831332857912284 23 1.8220673258991494E-4 5.1145749498923495E-5 0.03898584755555443 24 1.8220673258991494E-4 5.434235884260621E-5 0.04507538835527001 25 1.9499316996464581E-4 6.073557752997165E-5 0.05454694184060191 26 1.9499316996464581E-4 6.073557752997165E-5 0.07423166217900008 27 1.9499316996464581E-4 6.073557752997165E-5 0.28688290215814927 28 1.9499316996464581E-4 6.073557752997165E-5 0.5900589221410493 29 2.0777960733937668E-4 6.073557752997165E-5 0.8682598333217563 30 2.1097621668305942E-4 6.073557752997165E-5 1.1083347848603595 31 2.1417282602674212E-4 7.03254055610198E-5 1.3236935529536078 32 2.1417282602674212E-4 7.03254055610198E-5 1.575768575968396 33 2.2695926340147302E-4 7.03254055610198E-5 1.9574053722919884 34 2.3335248208883845E-4 7.03254055610198E-5 2.5475250300105676 35 2.461389194635693E-4 7.352201490470252E-5 3.412620220082078 36 2.461389194635693E-4 7.352201490470252E-5 4.574274448795065 37 2.589253568383002E-4 7.352201490470252E-5 5.9725353079087595 38 2.589253568383002E-4 7.352201490470252E-5 7.477045068387781 39 2.9408805961881007E-4 7.352201490470252E-5 8.985844678606023 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGAGC 13230 0.0 23.469578 45 CTCCGAG 21665 0.0 19.992085 45 CCTTTGG 20855 0.0 16.874624 1 CTTTGGG 20415 0.0 15.59408 2 AATTGAT 23265 0.0 14.583091 20 TAGACCG 6945 0.0 14.351044 5 TTTGGGA 22445 0.0 13.973201 3 TCTCCGA 31330 0.0 13.882285 44 GGGAAAC 23360 0.0 13.734053 6 TTGCATA 20580 0.0 13.568241 41 CCGTGCG 3560 0.0 13.397914 9 TGCATAA 20520 0.0 13.377665 42 TTGGGAA 24405 0.0 13.238182 4 ACCGTGC 3795 0.0 13.16111 8 ACATGAA 26820 0.0 12.985644 11 ATTGATT 26120 0.0 12.971969 21 CCTAGAC 2175 0.0 12.930147 3 ACGGTAC 5665 0.0 12.907281 8 CATGAAA 28670 0.0 12.736267 12 CTCGGGG 4460 0.0 12.612693 30 >>END_MODULE