FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n01 gdna_2000_dn_3_march.34100000003120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n01 gdna_2000_dn_3_march.34100000003120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31283147
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGGGAAACATGAAAAAATTGATTTTTTTTTGTTTTTTTGCATAAAAA320170.10234584135668959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC132300.023.46957845
CTCCGAG216650.019.99208545
CCTTTGG208550.016.8746241
CTTTGGG204150.015.594082
AATTGAT232650.014.58309120
TAGACCG69450.014.3510445
TTTGGGA224450.013.9732013
TCTCCGA313300.013.88228544
GGGAAAC233600.013.7340536
TTGCATA205800.013.56824141
CCGTGCG35600.013.3979149
TGCATAA205200.013.37766542
TTGGGAA244050.013.2381824
ACCGTGC37950.013.161118
ACATGAA268200.012.98564411
ATTGATT261200.012.97196921
CCTAGAC21750.012.9301473
ACGGTAC56650.012.9072818
CATGAAA286700.012.73626712
CTCGGGG44600.012.61269330
Produced by FastQC (version 0.11.2)