##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H3NH5BCXX l02n01 dnrfp 24-feb.3410000000303e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9872991 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.44686296179142 38.0 32.0 38.0 32.0 38.0 2 35.16802739919443 38.0 32.0 38.0 32.0 38.0 3 35.14926429083142 38.0 32.0 38.0 32.0 38.0 4 35.35071621153104 38.0 32.0 38.0 32.0 38.0 5 35.33025685934485 38.0 32.0 38.0 32.0 38.0 6 37.19677714686461 38.0 38.0 40.0 32.0 40.0 7 37.0305315785257 38.0 38.0 40.0 32.0 40.0 8 36.95455713471227 38.0 38.0 40.0 32.0 40.0 9 36.9115246838572 38.0 38.0 40.0 32.0 40.0 10 36.861320140978556 38.0 38.0 40.0 32.0 40.0 11 36.87891004863673 38.0 38.0 40.0 32.0 40.0 12 36.83670328474927 38.0 38.0 40.0 32.0 40.0 13 36.80459528424568 38.0 38.0 40.0 32.0 40.0 14 36.770207933948285 38.0 38.0 40.0 32.0 40.0 15 36.76715202110485 38.0 38.0 40.0 32.0 40.0 16 36.774203278418874 38.0 38.0 40.0 32.0 40.0 17 36.76046124219094 38.0 38.0 40.0 32.0 40.0 18 36.74187720823406 38.0 38.0 40.0 32.0 40.0 19 36.71368038317871 38.0 38.0 40.0 32.0 40.0 20 36.708394244459456 38.0 38.0 40.0 32.0 40.0 21 36.67816004288873 38.0 38.0 40.0 32.0 40.0 22 36.660193856147544 38.0 38.0 40.0 32.0 40.0 23 36.637016989076564 38.0 38.0 40.0 32.0 40.0 24 36.62183253281604 38.0 38.0 40.0 32.0 40.0 25 36.60046626194635 38.0 38.0 40.0 32.0 40.0 26 36.48154768904378 38.0 38.0 40.0 32.0 40.0 27 36.41838111672542 38.0 38.0 40.0 32.0 40.0 28 36.381464239155086 38.0 38.0 40.0 32.0 40.0 29 36.36702950504057 38.0 38.0 40.0 32.0 40.0 30 36.32183529793555 38.0 38.0 40.0 32.0 40.0 31 36.3142128864495 38.0 38.0 40.0 32.0 40.0 32 36.299339075666126 38.0 38.0 40.0 32.0 40.0 33 36.28231151026067 38.0 38.0 40.0 32.0 40.0 34 36.238564584936825 38.0 38.0 40.0 32.0 40.0 35 36.221541070988515 38.0 38.0 40.0 32.0 40.0 36 36.208489706918606 38.0 38.0 40.0 32.0 40.0 37 36.19173551358448 38.0 38.0 40.0 32.0 40.0 38 36.18476872915209 38.0 38.0 40.0 32.0 40.0 39 36.16557454574809 38.0 38.0 40.0 27.0 40.0 40 36.140572699802924 38.0 38.0 40.0 27.0 40.0 41 36.13481892164188 38.0 38.0 40.0 27.0 40.0 42 36.10407129916354 38.0 38.0 40.0 27.0 40.0 43 36.08562400188555 38.0 38.0 40.0 27.0 40.0 44 36.035722913147595 38.0 38.0 40.0 27.0 40.0 45 36.001812014211296 38.0 38.0 40.0 27.0 40.0 46 35.982757200933335 38.0 38.0 40.0 27.0 40.0 47 35.94465253741242 38.0 38.0 40.0 27.0 40.0 48 35.91430064101142 38.0 38.0 40.0 27.0 40.0 49 35.9102657948336 38.0 38.0 40.0 27.0 40.0 50 35.87589637223411 38.0 38.0 40.0 27.0 40.0 51 35.356950998942466 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.7365132976074591 2201 2 0.1402286571433038 2201 3 0.11565560671963482 2201 4 -0.06848247853968559 2201 5 -0.0849824476918144 2201 6 0.11908364908594393 2201 7 0.09280465008723837 2201 8 0.07344161781704628 2201 9 0.07257433429626303 2201 10 0.05487269949474438 2201 11 0.01636527778799035 2201 12 0.055744074652885445 2201 13 0.14244405239785607 2201 14 -0.15462872114203208 2201 15 -0.043733397750131076 2201 16 -0.16577733259894956 2201 17 -0.1486683459015481 2201 18 -0.213464627733984 2201 19 -0.14420307044791514 2201 20 -0.05355076097947631 2201 21 0.08261012812545943 2201 22 0.11543513568619801 2201 23 -0.12328065520421916 2201 24 -0.04578335823493518 2201 25 -0.09277814997789591 2201 26 0.011004991271057918 2201 27 -0.14118951829705395 2201 28 -0.19350017746718606 2201 29 -0.16784097099203876 2201 30 -0.06475878482020647 2201 31 -0.09800901043730192 2201 32 -0.2130353166100818 2201 33 -0.04028523807962614 2201 34 -0.16349229906373353 2201 35 0.028964164862344433 2201 36 -0.037971125146462725 2201 37 -0.020789648865843446 2201 38 -0.1091362170555783 2201 39 -0.1487651410774049 2201 40 -0.18193321321848543 2201 41 -0.19093505834785418 2201 42 -0.2025340347169049 2201 43 -0.18989134595555868 2201 44 -0.09992765644583557 2201 45 -0.0872846984867337 2201 46 -0.10106819526370003 2201 47 -0.1911462807074571 2201 48 -0.2729538003241814 2201 49 -0.08054894975155236 2201 50 -0.1969585645170966 2201 51 -0.13547618647514525 1101 1 0.7204510361791421 1101 2 0.10463271863774537 1101 3 0.06587108369976846 1101 4 0.02175237590020629 1101 5 0.07285057530902606 1101 6 0.17908592096785725 1101 7 0.12777979805020578 1101 8 0.1732285818001884 1101 9 0.120556125294776 1101 10 0.07734640479970523 1101 11 0.04431322263871351 1101 12 0.03193247034162994 1101 13 0.006406937112878097 1101 14 0.04913729008865175 1101 15 0.06281810221260287 1101 16 -0.01135941530935014 1101 17 -0.027065764641882595 1101 18 0.13765248370866345 1101 19 0.09862707784509439 1101 20 -0.03753531408759869 1101 21 0.06673905636332478 1101 22 0.05676078679393726 1101 23 0.049369688161917225 1101 24 -0.002978344827610613 1101 25 -0.03497105206370321 1101 26 0.018806084740340623 1101 27 0.006630242533574915 1101 28 -0.05842041772032758 1101 29 0.003866201484648002 1101 30 0.03284120596583051 1101 31 -0.03078964906733006 1101 32 -0.12935695230617483 1101 33 -0.14936372714002744 1101 34 -0.05601023678814698 1101 35 -0.10592745344564491 1101 36 -0.15420660945230225 1101 37 -0.08441272551397105 1101 38 -0.04635878534252669 1101 39 -0.08205247821942407 1101 40 -0.0785402915876503 1101 41 -0.24745177323084988 1101 42 -0.1442680443670099 1101 43 -0.07976813327378807 1101 44 -0.16531602907370058 1101 45 -0.1092644350926193 1101 46 -0.13222311771456674 1101 47 -0.17189317043855112 1101 48 -0.1644395537259271 1101 49 -0.14176528640539487 1101 50 -0.2325836820053624 1101 51 -0.15292654307335596 2202 1 0.5593171956937155 2202 2 0.029707347058966604 2202 3 0.07422688966764213 2202 4 -0.02974854665139759 2202 5 0.08906976143891399 2202 6 0.11431041586586588 2202 7 0.05503366424057532 2202 8 0.04311401306382123 2202 9 -0.07001032420383524 2202 10 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2202 45 -0.325327305398865 2202 46 -0.3530371238844765 2202 47 -0.2796861526621939 2202 48 -0.3028123564122609 2202 49 -0.3834746007654104 2202 50 -0.3032797001665344 2202 51 -0.32940082410331684 1102 1 0.6120861464480427 1102 2 0.04598485590060619 1102 3 -0.03415136617417858 1102 4 -0.16714468346947342 1102 5 -0.04701604727645048 1102 6 -0.059924926368601916 1102 7 -0.2124641249083723 1102 8 -0.05978310138100795 1102 9 -0.09522363492359176 1102 10 -0.07724485211886645 1102 11 -0.19911884011663972 1102 12 -0.19430292833463625 1102 13 -0.24329123259331453 1102 14 -0.19216699150098293 1102 15 -0.10664752257375909 1102 16 -0.1987048715746198 1102 17 -0.1574629697614256 1102 18 -0.19714629129524042 1102 19 -0.26859113417175706 1102 20 -0.1619403583211252 1102 21 -0.1273632510926248 1102 22 -0.24394398159870434 1102 23 -0.18217488590364184 1102 24 -0.2212435683357299 1102 25 -0.20267585486634232 1102 26 -0.09502120342251175 1102 27 -0.17975855502479732 1102 28 -0.26036823608826154 1102 29 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25 -0.004013655408009242 1213 26 0.06003143890252005 1213 27 -0.025354005382524747 1213 28 0.061304856547451436 1213 29 0.12969072958230043 1213 30 -0.040980113667693274 1213 31 0.19108516135692355 1213 32 0.023097828055583136 1213 33 0.07341613027125504 1213 34 0.05421723824336766 1213 35 0.050037446940109476 1213 36 0.16571485216725534 1213 37 0.045575431082234275 1213 38 0.030480837841366792 1213 39 0.11171504452669012 1213 40 0.141101440491326 1213 41 0.09329837773587002 1213 42 0.10313284989522487 1213 43 0.2458989850044233 1213 44 0.3065791811873595 1213 45 0.19567161171638503 1213 46 0.18589768659013828 1213 47 0.25303927616601385 1213 48 0.16215283295188954 1213 49 0.33613155980759046 1213 50 0.1840186528144372 1213 51 -0.018817976291742866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 7.0 14 18.0 15 52.0 16 141.0 17 249.0 18 411.0 19 944.0 20 2015.0 21 4577.0 22 10043.0 23 20489.0 24 36782.0 25 60517.0 26 90728.0 27 128832.0 28 170911.0 29 216799.0 30 268030.0 31 322282.0 32 381745.0 33 451066.0 34 540829.0 35 662092.0 36 859709.0 37 1220776.0 38 2012292.0 39 2410655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.752408922081557 30.091878468098187 15.990962886629498 24.16474972319076 2 15.093697694970363 23.023344027509438 39.124316632018214 22.75864164550199 3 16.606061931992038 28.552796209375657 32.241273186615885 22.599868672016413 4 13.42146161696659 21.169120980585753 40.354580735496036 25.05483666695162 5 13.045146404483344 38.43426358175183 35.81277809457925 12.707811919185577 6 24.544663031398652 42.071414014999434 19.62593999660485 13.757982956997067 7 21.80677567719853 33.90446724807102 26.11942014329801 18.16933693143243 8 21.314462861355793 38.01530863342224 22.82139222045275 17.848836284769227 9 20.985828914459663 17.725530186343732 29.857578113866406 31.431062785330198 10 16.443203483118744 28.038615653554228 34.014940355967106 21.503240507359926 11 30.441332317633023 28.843700961542456 24.915772737967654 15.799193982856865 12 17.787112335056314 28.464758045459575 32.9443124175845 20.80381720189961 13 24.49795609050996 25.171358912410636 28.18559239039112 22.145092606688287 14 19.10513237579169 25.04114507954074 29.228842607068113 26.624879937599456 15 21.61273113689661 32.74127364240481 25.19713630854115 20.44885891215742 16 20.271567147179613 32.80192395597241 27.206020951502946 19.720487945345035 17 19.212698563181107 31.507301080290663 29.752949232912297 19.527051123615934 18 19.295672405657008 31.390963488166857 29.991975076245893 19.32138902993024 19 19.64961783111116 31.306936266831396 30.050386959736926 18.993058942320516 20 19.722432644778063 30.630504980709496 30.303947405603832 19.343114968908612 21 19.867434296253283 30.457862262813773 30.060171228759348 19.614532212173597 22 20.1380108621592 30.180256418748886 29.91665848778754 19.765074231304375 23 19.73760535181284 30.631163342496713 29.753840553485766 19.877390752204676 24 19.945566647432376 30.724498786639227 29.52188450288266 19.808050063045737 25 19.97959888751038 30.353040937644938 29.70373415715663 19.963626017688053 26 19.69607177725003 30.97679942720487 29.6657881417674 19.661340653777696 27 19.669758379281255 30.700308205660455 29.78413854497231 19.84579487008598 28 19.484916933668785 30.625461047739506 30.049074774739275 19.840547243852434 29 19.56235116375246 30.383799120962777 30.166435274445153 19.887414440839606 30 19.59568181657039 30.534971795876903 30.375916545372224 19.493429842180486 31 19.673975150567248 30.13055762274577 30.13429527822692 20.06117194846006 32 19.644024562716428 30.39956248851573 30.030009527766765 19.92640342100108 33 19.54064356982744 30.45904747646102 30.030574261886787 19.96973469182475 34 19.60209505545276 30.37463713048797 30.040318046808316 19.98294976725095 35 19.729157353785357 30.252837320284815 30.08091811258804 19.93708721334179 36 19.477372242366915 30.37865259670373 30.3020231885755 19.84195197235385 37 19.68370953050852 30.282842767030573 30.12144899255763 19.911998709903276 38 19.517577758981346 30.278458444972323 30.400218803972912 19.803744992073423 39 19.333891488039434 30.520628678718147 30.382852596001847 19.76262723724057 40 19.439266631213755 30.078057180480638 30.806016865456204 19.676659322849403 41 19.509301821467425 30.385008093384897 30.326895766521545 19.77879431862613 42 19.370596807756215 30.194461419651326 30.7359088984963 19.699032874096165 43 19.49068544572595 29.84653465396681 30.622010587177172 20.040769313130063 44 19.275377571269384 30.065463615270165 30.69716739628546 19.96199141717499 45 19.313221775325722 29.909378870587606 30.803192129435487 19.974207224651185 46 19.32591974709329 29.931480797758198 30.79215166704398 19.95044778810453 47 19.20155413065892 29.96190743020355 30.87202883881121 19.96450960032632 48 19.068876355931184 29.956244943635625 31.162053952313194 19.812824748119997 49 19.02506390463067 30.1846113516665 30.896020105901993 19.89430463780083 50 18.765201929842988 30.109426039753 31.214970291758544 19.91040173864547 51 18.68474868806082 30.34269948008618 31.04714067654635 19.925411155306655 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37.0 1 64.0 2 91.0 3 175.5 4 260.0 5 918.0 6 1576.0 7 2327.5 8 3079.0 9 5626.0 10 8173.0 11 13905.0 12 19637.0 13 28073.5 14 36510.0 15 48680.5 16 60851.0 17 81337.5 18 101824.0 19 127868.0 20 153912.0 21 180659.0 22 207406.0 23 239544.5 24 271683.0 25 310492.0 26 387844.0 27 426387.0 28 472999.5 29 519612.0 30 542682.5 31 565753.0 32 589607.5 33 613462.0 34 631468.5 35 649475.0 36 655113.0 37 660751.0 38 666127.5 39 671504.0 40 658348.5 41 645193.0 42 624086.0 43 602979.0 44 578137.5 45 553296.0 46 517282.0 47 481268.0 48 445340.5 49 409413.0 50 377220.5 51 345028.0 52 317847.5 53 290667.0 54 262675.0 55 234683.0 56 213238.0 57 191793.0 58 174681.5 59 157570.0 60 144697.5 61 131825.0 62 120116.5 63 108408.0 64 98295.5 65 88183.0 66 83095.0 67 78007.0 68 72607.0 69 67207.0 70 59358.0 71 51509.0 72 44598.5 73 37688.0 74 32831.0 75 25012.5 76 22051.0 77 18200.0 78 14349.0 79 10483.5 80 6618.0 81 5057.0 82 3496.0 83 2478.5 84 1461.0 85 996.5 86 532.0 87 427.0 88 322.0 89 223.0 90 124.0 91 82.0 92 40.0 93 30.0 94 20.0 95 10.0 96 0.0 97 0.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07995550689755516 2 1.6205828608574645E-4 3 0.0 4 3.5450250081257037E-4 5 1.0128642880359153E-5 6 8.406773590698098E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 2.0257285760718307E-5 27 3.8488842945364783E-4 28 9.92607002275197E-4 29 0.0014585245747717182 30 0.0032512943645952885 31 0.0049832922971367035 32 0.00803201380412481 33 0.009743754450905506 34 0.009976713237153767 35 0.009237322306887548 36 0.008123171590048042 37 0.008741018805749949 38 0.011556781526489793 39 0.011911284027302365 40 0.007505324374346133 41 0.007404037945542541 42 0.007596482160269365 43 0.008710632877108873 44 0.00490226315409383 45 0.004649047082084851 46 0.005834098299086873 47 0.004679433010725929 48 0.0053884380123510696 49 0.004730076225127724 50 0.004841491296811676 51 0.00203585721895219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 9872991.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.61992352195871 #Duplication Level Percentage of deduplicated Percentage of total 1 44.116297473670244 12.184887622947524 2 12.321779827331108 6.806532329705976 3 9.995937078595706 8.282610529233743 4 8.363255459275022 9.239699047191191 5 6.639510981568975 9.169139276707003 6 5.011592582457298 8.305188231041166 7 3.5854508735065003 6.93209152425927 8 2.891512201268194 6.389067668947041 9 1.9871291957067743 4.939592077230518 >10 4.816076557778069 18.402979894289516 >50 0.16698079061621243 3.287868784691259 >100 0.09966491284684037 5.0392269498441715 >500 0.00425605970354971 0.7959691743131113 >1k 5.560056754003469E-4 0.22514688959857726 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.11107069782601847 2 0.0 0.0 0.11201266161389188 3 0.0 0.0 0.1123063922574223 4 0.0 0.0 0.13686835124229324 5 0.0 0.0 0.14155791289589953 6 0.0 0.0 0.14188202946807102 7 0.0 0.0 0.1424593621122515 8 0.0 0.0 0.14258090582681582 9 0.0 0.0 0.14438380425951974 10 5.0643214401795765E-5 0.0 0.14628798912102725 11 5.0643214401795765E-5 0.0 0.14719956698025957 12 5.0643214401795765E-5 5.0643214401795765E-5 0.14882014984111705 13 5.0643214401795765E-5 5.0643214401795765E-5 0.15000520105811907 14 5.0643214401795765E-5 5.0643214401795765E-5 0.1541376873533056 15 5.0643214401795765E-5 1.519296432053873E-4 0.15427948835363062 16 5.0643214401795765E-5 1.519296432053873E-4 0.15443141799683602 17 5.0643214401795765E-5 1.519296432053873E-4 0.1547352772832468 18 5.0643214401795765E-5 1.519296432053873E-4 0.15492772149797363 19 5.0643214401795765E-5 1.519296432053873E-4 0.15497836471237542 20 5.0643214401795765E-5 1.9244421472682392E-4 0.15505939385541828 21 5.0643214401795765E-5 1.9244421472682392E-4 0.15519106621286294 22 5.0643214401795765E-5 1.9244421472682392E-4 0.15572788428552198 23 5.0643214401795765E-5 1.9244421472682392E-4 0.15599122900041132 24 5.0643214401795765E-5 1.9244421472682392E-4 0.1569939646455669 25 6.0771857282154924E-5 1.9244421472682392E-4 0.1577536128615938 26 6.0771857282154924E-5 1.9244421472682392E-4 0.15895892136435655 27 6.0771857282154924E-5 1.9244421472682392E-4 0.16399285687589504 28 6.0771857282154924E-5 1.9244421472682392E-4 0.17809192776535498 29 6.0771857282154924E-5 1.9244421472682392E-4 0.19675901659385692 30 6.0771857282154924E-5 1.9244421472682392E-4 0.2142714401339979 31 6.0771857282154924E-5 2.9373064353041545E-4 0.23248274003288366 32 6.0771857282154924E-5 2.9373064353041545E-4 0.25906029895094607 33 1.0128642880359153E-4 3.038592864107746E-4 0.2901451039507683 34 1.1141507168395069E-4 3.038592864107746E-4 0.33623042905640244 35 1.1141507168395069E-4 3.038592864107746E-4 0.39784296369762717 36 1.1141507168395069E-4 3.342452150518521E-4 0.4882411014048326 37 1.1141507168395069E-4 3.342452150518521E-4 0.6155074991965453 38 1.1141507168395069E-4 3.342452150518521E-4 0.766768651971829 39 1.8231557184646477E-4 3.342452150518521E-4 0.9322504193511368 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGCT 1670 0.0 14.956375 36 GCTACGC 1690 0.0 14.646228 35 TACGCTA 1710 0.0 14.474928 37 GACGCTA 1785 0.0 14.244633 32 CGCTACG 1830 0.0 14.017597 34 GAGACGC 1855 0.0 13.70696 30 ACGCTAC 2025 0.0 13.112237 33 CGAGACG 2975 0.0 13.08476 29 TCCGAGC 3125 0.0 13.030941 19 CTCCGAG 3130 0.0 12.938246 18 AGACGCT 2045 0.0 12.653514 31 TATCTCG 2550 0.0 12.441551 42 CCGAGCC 3355 0.0 12.338789 20 CTCGTAT 3195 0.0 12.323815 45 AGCCCAC 3310 0.0 12.234841 23 GCCCACG 3295 0.0 12.085941 24 CGAGCCC 3335 0.0 12.07548 21 GAGCCCA 3330 0.0 12.026234 22 CCACGAG 3390 0.0 11.681472 26 CCCACGA 3455 0.0 11.65678 25 >>END_MODULE