FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n01 dnrfp 24-feb.3410000000303e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n01 dnrfp 24-feb.3410000000303e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9872991
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT16700.014.95637536
GCTACGC16900.014.64622835
TACGCTA17100.014.47492837
GACGCTA17850.014.24463332
CGCTACG18300.014.01759734
GAGACGC18550.013.7069630
ACGCTAC20250.013.11223733
CGAGACG29750.013.0847629
TCCGAGC31250.013.03094119
CTCCGAG31300.012.93824618
AGACGCT20450.012.65351431
TATCTCG25500.012.44155142
CCGAGCC33550.012.33878920
CTCGTAT31950.012.32381545
AGCCCAC33100.012.23484123
GCCCACG32950.012.08594124
CGAGCCC33350.012.0754821
GAGCCCA33300.012.02623422
CCACGAG33900.011.68147226
CCCACGA34550.011.6567825
Produced by FastQC (version 0.11.2)