##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H3NH5BCXX l02n01 dn_rfp_3_march.341000000030b4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42496397
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.8051024890416	38.0	32.0	38.0	27.0	38.0
2	34.67720404626303	38.0	32.0	38.0	32.0	38.0
3	34.6743555694851	38.0	32.0	38.0	32.0	38.0
4	34.95441893579825	38.0	32.0	38.0	32.0	38.0
5	34.94336336325171	38.0	32.0	38.0	32.0	38.0
6	36.72442939103755	38.0	38.0	40.0	32.0	40.0
7	36.538902792159064	38.0	38.0	40.0	32.0	40.0
8	36.45776344286317	38.0	38.0	40.0	32.0	40.0
9	36.46445657498917	38.0	38.0	40.0	32.0	40.0
10	36.403252209828516	38.0	38.0	40.0	32.0	40.0
11	36.44685567108195	38.0	38.0	40.0	32.0	40.0
12	36.377325588331644	38.0	38.0	40.0	32.0	40.0
13	36.36034064252553	38.0	38.0	40.0	32.0	40.0
14	36.31719458475503	38.0	38.0	40.0	32.0	40.0
15	36.309860080608715	38.0	38.0	40.0	32.0	40.0
16	36.320682998137464	38.0	38.0	40.0	32.0	40.0
17	36.30357392416115	38.0	38.0	40.0	32.0	40.0
18	36.29057329260172	38.0	38.0	40.0	32.0	40.0
19	36.26199331675107	38.0	38.0	40.0	32.0	40.0
20	36.253205583522764	38.0	38.0	40.0	32.0	40.0
21	36.222538065991806	38.0	38.0	40.0	32.0	40.0
22	36.20500121457356	38.0	38.0	40.0	32.0	40.0
23	36.176206349917145	38.0	38.0	40.0	32.0	40.0
24	36.15732211368413	38.0	38.0	40.0	32.0	40.0
25	36.13374618558839	38.0	38.0	40.0	32.0	40.0
26	35.98731068895088	38.0	38.0	40.0	27.0	40.0
27	35.913391198787984	38.0	38.0	40.0	27.0	40.0
28	35.884652785976186	38.0	38.0	40.0	27.0	40.0
29	35.8640742178684	38.0	38.0	40.0	27.0	40.0
30	35.810789888846344	38.0	38.0	40.0	27.0	40.0
31	35.77036636776525	38.0	38.0	40.0	27.0	40.0
32	35.76200394118118	38.0	38.0	40.0	27.0	40.0
33	35.72224774726196	38.0	38.0	40.0	27.0	40.0
34	35.66195677718278	38.0	38.0	40.0	27.0	40.0
35	35.622509856541484	38.0	38.0	40.0	27.0	40.0
36	35.57981235444501	38.0	38.0	40.0	27.0	40.0
37	35.554733381279355	38.0	38.0	40.0	27.0	40.0
38	35.527897435634365	38.0	38.0	40.0	27.0	40.0
39	35.50227095722962	38.0	38.0	40.0	27.0	40.0
40	35.46852680240163	38.0	38.0	40.0	27.0	40.0
41	35.4476840471911	38.0	38.0	40.0	27.0	40.0
42	35.4177504036401	38.0	38.0	40.0	27.0	40.0
43	35.39813398768842	38.0	38.0	40.0	27.0	40.0
44	35.29050258072467	38.0	38.0	40.0	27.0	40.0
45	35.304888506195006	38.0	38.0	40.0	27.0	40.0
46	35.3265022444138	38.0	38.0	40.0	27.0	40.0
47	35.31325938055407	38.0	38.0	40.0	27.0	40.0
48	35.311128423428464	38.0	38.0	40.0	27.0	40.0
49	35.33374579967332	38.0	38.0	40.0	27.0	40.0
50	35.320876402768924	38.0	38.0	40.0	27.0	40.0
51	34.61228522502743	38.0	32.0	38.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.9398430280320014
2201	2	0.22397903738513492
2201	3	0.23825579740154978
2201	4	-0.029584247921391693
2201	5	0.03817502086549496
2201	6	0.17283397207769724
2201	7	0.20547304938484956
2201	8	0.16267016465076267
2201	9	0.11233806987277006
2201	10	0.17375619742651338
2201	11	0.093979698915021
2201	12	0.08205622321592188
2201	13	0.18962617210914345
2201	14	-0.0330247762629341
2201	15	-0.0448589979874896
2201	16	-0.04247579182928263
2201	17	-0.09882638166683222
2201	18	-0.14619288223762794
2201	19	-0.13476422156350765
2201	20	-0.05960407752938579
2201	21	0.051781431704199576
2201	22	0.08310085803797307
2201	23	-0.06159646216377013
2201	24	-0.012903353448805888
2201	25	-0.084432513130416
2201	26	0.06331898306599726
2201	27	-0.13045375326792197
2201	28	-0.15653307422901008
2201	29	-0.15115925821442744
2201	30	-0.0259855606620647
2201	31	-0.005433952597869052
2201	32	-0.0946638019068402
2201	33	-0.0572097515267842
2201	34	-0.10821421423071342
2201	35	0.03464501584684143
2201	36	0.06925847304491128
2201	37	0.08170462868663719
2201	38	-0.045069974758710885
2201	39	-0.21102189641637636
2201	40	-0.18036357253642876
2201	41	-0.010839302521432614
2201	42	-0.13956231809276431
2201	43	-0.19601646224611358
2201	44	-0.16263383515313734
2201	45	-0.09750436946438867
2201	46	-0.26310336199311024
2201	47	-0.13793617941804825
2201	48	-0.28404739043329386
2201	49	-0.11291700405465122
2201	50	-0.17313166472817443
2201	51	-0.13387043696252476
1101	1	0.8792639434057037
1101	2	0.1772666682659434
1101	3	0.10966057077011016
1101	4	0.023206955796922557
1101	5	0.10878452578052134
1101	6	0.10719623816221713
1101	7	0.1942161157904394
1101	8	0.16671962987507527
1101	9	0.17876721518571514
1101	10	0.13140397251337532
1101	11	0.11312405269164572
1101	12	0.008192225216291149
1101	13	0.04719904162446653
1101	14	0.05462900828292305
1101	15	0.0999648481515365
1101	16	0.05957374037065932
1101	17	0.0719641339622541
1101	18	0.13927755398021446
1101	19	0.08209263284094703
1101	20	-0.07290453327158275
1101	21	-0.0016258726176516802
1101	22	0.09825023118953169
1101	23	0.06654137304169438
1101	24	0.030640992327562344
1101	25	0.027942311381423224
1101	26	-0.007296069791138393
1101	27	0.004516397409517481
1101	28	0.030865686727196362
1101	29	0.048794085842928325
1101	30	0.03458259380960271
1101	31	-0.04538869052541372
1101	32	-0.07227636683795424
1101	33	-0.0757223732458101
1101	34	0.009039636474923896
1101	35	-0.04889387428058711
1101	36	-0.08389959933629143
1101	37	-0.08701386974825454
1101	38	-0.08279912659737931
1101	39	-0.08789868219329122
1101	40	-0.09347073935753514
1101	41	-0.2876036988500843
1101	42	-0.16662620138024664
1101	43	-0.09662101774053156
1101	44	-0.2853258394472675
1101	45	-0.2455315286832871
1101	46	-0.15806009443061697
1101	47	-0.2838092219855568
1101	48	-0.29491103395978513
1101	49	-0.2557146272371682
1101	50	-0.3208395032562805
1101	51	-0.2738390764250056
2202	1	0.6904392368778929
2202	2	0.16340312114490274
2202	3	0.11702912562828516
2202	4	0.011505926049395043
2202	5	0.13821726304266946
2202	6	0.14873500264914696
2202	7	0.17585489689258083
2202	8	0.0952310153371343
2202	9	-0.018325724199094395
2202	10	0.03643241123947405
2202	11	-0.0878170219175658
2202	12	0.06006296675727896
2202	13	0.011261499837885935
2202	14	0.12843613879138616
2202	15	0.02075306303807878
2202	16	0.06128476369222824
2202	17	0.02058727418208406
2202	18	-0.0748532929163872
2202	19	-0.07870322024191978
2202	20	-0.08900294947400056
2202	21	-0.0022817895909028607
2202	22	-0.06685311381986736
2202	23	-0.008937023858187843
2202	24	0.010626361446817612
2202	25	0.07048063601167343
2202	26	0.019753695580668307
2202	27	-0.04818885052452515
2202	28	0.013609319644409368
2202	29	-0.06742450533051425
2202	30	-0.2210960559281432
2202	31	-0.13867787455602354
2202	32	-0.20311287679037804
2202	33	-0.13132660080311354
2202	34	-0.10702571045688813
2202	35	-0.10535969936066181
2202	36	-0.10124553601221464
2202	37	-0.06872408873545766
2202	38	-0.14634387694402307
2202	39	-0.24218057227274414
2202	40	-0.15378238852566284
2202	41	-0.168661159984687
2202	42	-0.11696248988759095
2202	43	-0.3398382902101318
2202	44	-0.17707113671380625
2202	45	-0.2473210339886549
2202	46	-0.2346032937277016
2202	47	-0.24713058762217344
2202	48	-0.27033136914511147
2202	49	-0.3219017577880052
2202	50	-0.18664825842064658
2202	51	-0.29683277632967986
1102	1	0.7160373777310127
1102	2	0.04577097626243898
1102	3	-0.021110423004607526
1102	4	-0.1694231542717901
1102	5	-0.08697782412856014
1102	6	-0.09685341216228949
1102	7	-0.15535431603333905
1102	8	-0.05189591714776043
1102	9	-0.11817082649925226
1102	10	-0.08620998049286754
1102	11	-0.17699074956420446
1102	12	-0.14965516075050544
1102	13	-0.23157602571149738
1102	14	-0.1828283049071544
1102	15	-0.16415317724322165
1102	16	-0.16138370025272764
1102	17	-0.1851144856989677
1102	18	-0.18917992828787789
1102	19	-0.25750265589782373
1102	20	-0.19782466856741365
1102	21	-0.17303199986850615
1102	22	-0.17989782830949252
1102	23	-0.1863178517952946
1102	24	-0.22940165050867733
1102	25	-0.24675677033360444
1102	26	-0.21596384351995113
1102	27	-0.28487367962158316
1102	28	-0.3023070497427014
1102	29	-0.2980187797138427
1102	30	-0.3539254402432661
1102	31	-0.313494411235979
1102	32	-0.347324642739288
1102	33	-0.3294621829128488
1102	34	-0.27392751743594346
1102	35	-0.30878129397572707
1102	36	-0.4181382065302799
1102	37	-0.27991818584573736
1102	38	-0.3501823897442975
1102	39	-0.4071300756855223
1102	40	-0.3714423170789658
1102	41	-0.3329053350172586
1102	42	-0.4663652822166142
1102	43	-0.45791451621275314
1102	44	-0.3695888313190352
1102	45	-0.5113422720359679
1102	46	-0.48467190188812026
1102	47	-0.49095934956679343
1102	48	-0.6265642084836784
1102	49	-0.6423241656700398
1102	50	-0.573193982696246
1102	51	-0.5226648336003663
2203	1	0.5906403955148889
2203	2	-0.015931738242009885
2203	3	-0.03652007724470252
2203	4	-0.25130102046250613
2203	5	0.02018804229978599
2203	6	-0.23164738525851902
2203	7	-0.31636756877902883
2203	8	-0.5467427294526885
2203	9	-0.21389899281098224
2203	10	-0.24469714192947833
2203	11	-0.17996589221627346
2203	12	-0.1518077406041911
2203	13	-0.14002267783347122
2203	14	-0.10192223430054526
2203	15	-0.14597754631906668
2203	16	-0.21129985587182176
2203	17	-0.16003398527823975
2203	18	-0.1339953063663728
2203	19	-0.12929096487172842
2203	20	-0.10222059800259586
2203	21	-0.1194662474406627
2203	22	-0.15213049642993326
2203	23	-0.285692750710588
2203	24	-0.1500428737286299
2203	25	-0.13040339952405589
2203	26	-0.24289192619941957
2203	27	-0.21281887115029008
2203	28	-0.2844231146351959
2203	29	-0.4045251606768545
2203	30	-0.27327211028174503
2203	31	-0.19088644938643284
2203	32	-0.26175674179536657
2203	33	-0.27046125306541313
2203	34	-0.3018071445980226
2203	35	-0.3122612092957908
2203	36	-0.4270828706581611
2203	37	-0.3434904681839086
2203	38	-0.42437504406140647
2203	39	-0.30840254200222716
2203	40	-0.2952850811375711
2203	41	-0.34867515321690234
2203	42	-0.380140320108012
2203	43	-0.29470361117819976
2203	44	-0.4347558110065606
2203	45	-0.3650773164388852
2203	46	-0.290441572704502
2203	47	-0.3819788393662762
2203	48	-0.4954666198018671
2203	49	-0.4078387224090818
2203	50	-0.38050775574546947
2203	51	-0.4547929289673789
1103	1	0.611443338374194
1103	2	0.04620209755255189
1103	3	-0.09075139001681976
1103	4	-0.16995754476411662
1103	5	-0.1870607250291556
1103	6	-0.07242333036653292
1103	7	-0.049730909221288755
1103	8	-0.038253075299536476
1103	9	-0.09080870094560112
1103	10	-0.13304293233739628
1103	11	-0.16452794301164175
1103	12	-0.18392338742982162
1103	13	-0.1880837001957829
1103	14	-0.2063846580640103
1103	15	-0.1824924740577245
1103	16	-0.22026558458995282
1103	17	-0.2302794412953162
1103	18	-0.22466352481023222
1103	19	-0.24996948712664135
1103	20	-0.2245617553835899
1103	21	-0.2704788079721254
1103	22	-0.3137910949418199
1103	23	-0.23133545202329486
1103	24	-0.2370971107576807
1103	25	-0.2711056474281506
1103	26	-0.27585143782125954
1103	27	-0.1100450810779563
1103	28	-0.24250443473805205
1103	29	-0.21294571030900045
1103	30	-0.40135607281952446
1103	31	-0.3637337928539992
1103	32	-0.33036175492659225
1103	33	-0.3436591736731174
1103	34	-0.2517974352332004
1103	35	-0.2857446159324084
1103	36	-0.5143503585735303
1103	37	-0.35902509894462753
1103	38	-0.34834691383938576
1103	39	-0.32601477241384913
1103	40	-0.3598837806315913
1103	41	-0.35941046815563027
1103	42	-0.3759803118215501
1103	43	-0.4344451974660757
1103	44	-0.39742411879049655
1103	45	-0.4418641080121688
1103	46	-0.46397769847155246
1103	47	-0.4171798173310748
1103	48	-0.3982528908050256
1103	49	-0.4393553130047607
1103	50	-0.37703847034750027
1103	51	-0.5406685310831989
2204	1	0.5965591035524156
2204	2	-0.01643311675217518
2204	3	-0.024838650920486316
2204	4	-0.16743642343768528
2204	5	-0.1852452596220857
2204	6	-0.12328459672450265
2204	7	-0.172962146164771
2204	8	-0.19642353174663185
2204	9	-0.24862588082437043
2204	10	-0.34707132742229874
2204	11	-0.3398769493335365
2204	12	-0.2782211349115471
2204	13	-0.3040238646653677
2204	14	-0.3465762010093769
2204	15	-0.29613049697121596
2204	16	-0.3328321209443601
2204	17	-0.29538762045656597
2204	18	-0.2800063168319298
2204	19	-0.24155286004723564
2204	20	-0.26441524756786805
2204	21	-0.3026980271331823
2204	22	-0.3995602618456928
2204	23	-0.38210447528278024
2204	24	-0.41616093820795896
2204	25	-0.33457521953864955
2204	26	-0.3535857475845816
2204	27	-0.3927526772172527
2204	28	-0.3919517035802471
2204	29	-0.3721267958931236
2204	30	-0.4580899534421192
2204	31	-0.396476540293655
2204	32	-0.3644398624214915
2204	33	-0.46847100126738894
2204	34	-0.44030678140427426
2204	35	-0.4286748683417372
2204	36	-0.44187896359832024
2204	37	-0.44994666507574266
2204	38	-0.4121648223462273
2204	39	-0.41959865857062084
2204	40	-0.38353924102779047
2204	41	-0.5198771321767808
2204	42	-0.7912836969845287
2204	43	-0.6992875787444888
2204	44	-0.5235452557709337
2204	45	-0.44527489147854027
2204	46	-0.42205849791704964
2204	47	-0.38248178397460464
2204	48	-0.4422016775227817
2204	49	-0.5380020648665749
2204	50	-0.49113596311313046
2204	51	-0.5389375589482697
2205	1	0.681762995608004
2205	2	-0.010040327555735473
2205	3	0.09063214912918482
2205	4	-0.1396092583852635
2205	5	-0.11641768021446808
2205	6	-0.17682277623099196
2205	7	-0.2569626967781886
2205	8	-0.25666976383483586
2205	9	-0.38509109149678267
2205	10	-0.346760393607056
2205	11	-0.32224394061719863
2205	12	-0.2708501140119566
2205	13	-0.30664896528950436
2205	14	-0.2584038794716008
2205	15	-0.33452198497142405
2205	16	-0.3698626869647228
2205	17	-0.34184961301677674
2205	18	-0.3625206902138629
2205	19	-0.372566514236901
2205	20	-0.4613042957088709
2205	21	-0.41090302274083257
2205	22	-0.45169185659235467
2205	23	-0.37046040128294067
2205	24	-0.3195269766141706
2205	25	-0.34375372664151627
2205	26	-0.3732162924918967
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1210	13	0.3277772626414617
1210	14	0.27338847270321054
1210	15	0.3742993887945616
1210	16	0.3696373542197193
1210	17	0.3921796976827494
1210	18	0.4073321875066753
1210	19	0.41875647581918685
1210	20	0.39724752740242053
1210	21	0.4319445995307589
1210	22	0.47428286197598624
1210	23	0.5015910882250836
1210	24	0.548028981514129
1210	25	0.47713725033717935
1210	26	0.4122813950240811
1210	27	0.47062366632575703
1210	28	0.5289944278224397
1210	29	0.48510969031770657
1210	30	0.5732483428431436
1210	31	0.6180569293212201
1210	32	0.5463704959216855
1210	33	0.5615706177464688
1210	34	0.5928722077966739
1210	35	0.5487065070869548
1210	36	0.608325708085907
1210	37	0.6606677610752882
1210	38	0.649717142132161
1210	39	0.7018440660891585
1210	40	0.6743281288327125
1210	41	0.6675172971900025
1210	42	0.6315249374583516
1210	43	0.6266884016338068
1210	44	0.6339284341947504
1210	45	0.7744783921174587
1210	46	0.7124916921324669
1210	47	0.7177770160084123
1210	48	0.7656421877367023
1210	49	0.6481756009941364
1210	50	0.6995229816516328
1210	51	0.8981498571534914
1211	1	-0.17178707207653332
1211	2	0.139368661633533
1211	3	0.17978680592016616
1211	4	0.1481701653148093
1211	5	-2.024045921115416
1211	6	-0.649383825772567
1211	7	-0.21458870932708862
1211	8	0.13084825243851128
1211	9	0.2213050262237033
1211	10	0.28208038749925635
1211	11	0.2989680582213907
1211	12	0.38529394197060896
1211	13	0.32206724392166564
1211	14	0.4069251561724556
1211	15	0.43579324676107944
1211	16	0.44877080546770287
1211	17	0.35197784026418333
1211	18	0.45298788733516915
1211	19	0.3067817812134592
1211	20	0.27565494171748384
1211	21	0.46333592455708583
1211	22	0.4413655690836862
1211	23	0.4933132129762967
1211	24	0.4908625657181247
1211	25	0.44278370158123437
1211	26	0.4826310562125684
1211	27	0.5116712203835689
1211	28	0.4694260320192072
1211	29	0.5203606541416761
1211	30	0.39231242206778205
1211	31	0.4443406964581271
1211	32	0.5469829147490017
1211	33	0.5966776788686161
1211	34	0.6793774176157541
1211	35	0.698557805302471
1211	36	0.6090198548575572
1211	37	0.632244283287406
1211	38	0.6131604531161798
1211	39	0.7013465760797857
1211	40	0.668898023600029
1211	41	0.6619301160972881
1211	42	0.8022378267351371
1211	43	0.7695509211374372
1211	44	0.8682141222969335
1211	45	0.7952086629699195
1211	46	0.7402531079588002
1211	47	0.7675171548557529
1211	48	0.846790425964187
1211	49	0.8046127613366423
1211	50	0.8145647155112243
1211	51	0.9072215343921854
1208	1	-0.32819069039808824
1208	2	0.01878487692796682
1208	3	0.1780600478483123
1208	4	0.25094761311198255
1208	5	0.3351671588701777
1208	6	0.2968355703946983
1208	7	0.25661722358496064
1208	8	0.09048474567953946
1208	9	0.17961183791298652
1208	10	0.16932725110598312
1208	11	0.2208320034255351
1208	12	0.2587892325408774
1208	13	0.3046571465293013
1208	14	0.34549265255235895
1208	15	0.24956572583597136
1208	16	0.23983546130597944
1208	17	0.31969238373997655
1208	18	0.3674493550016962
1208	19	0.3727058129205787
1208	20	0.32511524883765475
1208	21	0.3824506674261201
1208	22	0.4502166693313967
1208	23	0.3951468222138388
1208	24	0.41315265898674625
1208	25	0.43539839662685154
1208	26	0.3958087669437944
1208	27	0.3772576803050782
1208	28	0.3730685041660067
1208	29	0.4766146377796616
1208	30	0.44365457885249526
1208	31	0.48876394721363425
1208	32	0.49507897691430003
1208	33	0.5372543728489916
1208	34	-0.583638864121113
1208	35	-0.11851695390376449
1208	36	0.1816785452599774
1208	37	0.3144590208111282
1208	38	0.3927806722179099
1208	39	0.5626085989482945
1208	40	0.46372392077535807
1208	41	0.6096384561317834
1208	42	0.6190080276616641
1208	43	0.5991550151521281
1208	44	0.6649409701059454
1208	45	0.7218360333113409
1208	46	0.583476114729315
1208	47	0.6290345201643888
1208	48	0.6993062076214045
1208	49	0.6637154662005997
1208	50	0.6513788251750583
1208	51	0.7117504240375894
1209	1	-0.7772136628180419
1209	2	0.08570540640666735
1209	3	0.1550326418167387
1209	4	0.22159717232310783
1209	5	0.029338162973708393
1209	6	0.07608612763464606
1209	7	0.1833000190132168
1209	8	0.2823135105266843
1209	9	0.24739251541134166
1209	10	0.2981166945923235
1209	11	0.24861338058591542
1209	12	0.261028776667942
1209	13	0.2654357081856844
1209	14	0.14933152351427026
1209	15	0.22249681627960172
1209	16	0.2920391248535097
1209	17	0.3036501430414802
1209	18	0.27054208545342817
1209	19	0.3246374536700074
1209	20	0.3009775705035196
1209	21	0.3025681290602904
1209	22	0.34483349672548513
1209	23	0.41968660324449303
1209	24	0.27989004477682045
1209	25	0.30588566590139266
1209	26	0.33816410280030595
1209	27	0.4037170476936609
1209	28	0.3133653903714517
1209	29	0.37559069704087733
1209	30	0.48538303526041204
1209	31	0.3531107923607877
1209	32	0.3856478827246832
1209	33	0.49155346248089415
1209	34	0.537165619396454
1209	35	0.5184197979627356
1209	36	0.5077752898254744
1209	37	0.5029574698776855
1209	38	0.5423664247234541
1209	39	0.6411156965383285
1209	40	0.674310175761093
1209	41	0.654211794236609
1209	42	0.5766401933643692
1209	43	0.6699381815098278
1209	44	0.658520417734259
1209	45	0.7840550435615228
1209	46	0.7635756389276054
1209	47	0.7614771930719257
1209	48	0.7117713299446535
1209	49	0.697732770216632
1209	50	0.7531712256384964
1209	51	0.9091017714134537
1214	1	-0.8085827428850934
1214	2	0.01815798951756875
1214	3	0.18547503094845297
1214	4	0.19438968559705216
1214	5	0.16058801996442185
1214	6	0.09986019011382297
1214	7	0.1604712733315452
1214	8	0.23335149734185023
1214	9	0.21969880173177359
1214	10	0.11284843652509124
1214	11	0.2093418661025055
1214	12	0.23680711129425447
1214	13	0.16966470857267524
1214	14	0.12119971624913006
1214	15	0.15400429052250075
1214	16	0.21481829644687878
1214	17	0.26466804793353305
1214	18	0.18630635813139662
1214	19	0.18585527676536628
1214	20	0.23943980583098323
1214	21	0.2307709243794065
1214	22	0.26255182611529904
1214	23	0.30886490432115465
1214	24	0.34824714836367576
1214	25	0.22137436268765498
1214	26	0.24554694543419942
1214	27	0.28108236584482427
1214	28	0.3282594910475467
1214	29	0.37709133807365447
1214	30	0.36696886935100537
1214	31	0.23560364725642557
1214	32	0.337903148876471
1214	33	0.36305357934651283
1214	34	0.530087662395978
1214	35	0.46517857265214957
1214	36	0.3507920091312613
1214	37	0.457283122363215
1214	38	0.5186210334114136
1214	39	0.5136957298950264
1214	40	0.5002953161397485
1214	41	0.4613196625881457
1214	42	0.584336948544184
1214	43	0.5149107595831026
1214	44	0.5108463947006996
1214	45	0.5657313787341067
1214	46	0.4764403643595756
1214	47	0.6056851358381934
1214	48	0.615548688984255
1214	49	0.6187091792198984
1214	50	0.6580249109198846
1214	51	0.7143098203069371
1215	1	-0.5265069842214487
1215	2	-0.0640399208966258
1215	3	0.02635983755677529
1215	4	0.10975762293151092
1215	5	0.06867359335699774
1215	6	0.08748606071331011
1215	7	-0.07632869422064914
1215	8	0.025759949320502074
1215	9	-0.11286598528108982
1215	10	0.05727603453166097
1215	11	-0.04084857980706147
1215	12	0.12420433472624381
1215	13	0.032576542113524454
1215	14	0.13433866890063229
1215	15	0.06652701965340668
1215	16	0.160349104775257
1215	17	0.17050871907113674
1215	18	0.06937392322774372
1215	19	0.1640910917856715
1215	20	0.09102753728160451
1215	21	-0.01380505868133497
1215	22	0.0633661853326899
1215	23	0.017114372062998484
1215	24	0.009840638234372534
1215	25	0.1527199732114397
1215	26	0.04988415697462756
1215	27	0.1286062412879403
1215	28	-0.06454760830360584
1215	29	0.05432082856908238
1215	30	0.15967798158680324
1215	31	0.19971106463096078
1215	32	0.13591898053225293
1215	33	0.2887946267974755
1215	34	0.1356938067862643
1215	35	0.23551075531875654
1215	36	0.27079641208502636
1215	37	0.18172151188532837
1215	38	0.29770876781914524
1215	39	0.052183088004419176
1215	40	0.18461771962806495
1215	41	0.4402485142090953
1215	42	0.3331444106948922
1215	43	0.4417420967554122
1215	44	0.4195289460702796
1215	45	0.39391049635916175
1215	46	0.593696444906719
1215	47	0.4251980194470377
1215	48	0.49869459456400733
1215	49	0.4373881813086271
1215	50	0.5878200885979155
1215	51	0.7278200574448306
1212	1	0.4610592986378421
1212	2	-0.20544215800615007
1212	3	0.01414955410976404
1212	4	0.06653969635713253
1212	5	0.04647547987932654
1212	6	-0.35252036349250915
1212	7	-0.24895413381658216
1212	8	-0.22491646097535778
1212	9	-0.09622758065101777
1212	10	-0.23591111003051424
1212	11	-0.17585897796715955
1212	12	-0.13404191047755631
1212	13	-0.20860865099217563
1212	14	-0.37696921058066835
1212	15	-0.3756326546644644
1212	16	-0.5046657086323592
1212	17	-0.5813680125258571
1212	18	-0.4160210518347398
1212	19	-0.6554753756605365
1212	20	-0.2535084997183361
1212	21	-0.2820735102334808
1212	22	-0.23678462898635644
1212	23	-0.2779732415347098
1212	24	-0.5508401356322921
1212	25	-0.48249279649819243
1212	26	-0.3999206934893351
1212	27	-0.38295925291488686
1212	28	-0.09655678311676041
1212	29	-0.06377114625065872
1212	30	0.024241232078779262
1212	31	0.05952981066669594
1212	32	-0.14728113415750244
1212	33	-0.1711352306865379
1212	34	-0.42206331657801854
1212	35	-0.5902757362050863
1212	36	-0.2693214319679882
1212	37	-0.6302907244012701
1212	38	-0.4475725316126642
1212	39	-0.23810860599981254
1212	40	-0.14577881044540675
1212	41	-0.16660541581195076
1212	42	-0.0433784969835358
1212	43	-0.055720308545552655
1212	44	-0.06614676965641308
1212	45	-0.028263605390499436
1212	46	0.03864618571757461
1212	47	0.1389407228235342
1212	48	-0.11663423327727429
1212	49	-0.3347848656990422
1212	50	-0.4796545014472642
1212	51	-0.5609912201109992
1213	1	-0.24989898499647722
1213	2	-0.21616152544824274
1213	3	-0.17362982101514035
1213	4	-0.18751408761858102
1213	5	-0.13276805991580432
1213	6	-0.2560250658761092
1213	7	-0.250382305002816
1213	8	-0.2774329438181127
1213	9	-0.21688699844140302
1213	10	-0.48002518946272943
1213	11	-0.46476272655964834
1213	12	-0.5893858285489912
1213	13	-0.40005497505374166
1213	14	-0.36992739215175874
1213	15	-0.46489047244500625
1213	16	-0.4666557250994643
1213	17	-0.46688286164553006
1213	18	-0.35925948916680284
1213	19	-0.46284906036875384
1213	20	-0.4000082488873815
1213	21	-0.32843145494209836
1213	22	-0.37439902226052624
1213	23	-0.5717659269440531
1213	24	-0.38728892831532136
1213	25	-0.27549340573744274
1213	26	-0.2680044283243177
1213	27	-0.44135509632773307
1213	28	-0.32295143801390935
1213	29	-0.16394345814668299
1213	30	-0.43562810926781736
1213	31	-0.12461634881811534
1213	32	-0.3840071426724876
1213	33	-0.31938878710185037
1213	34	-0.2913457515424227
1213	35	-0.33322154997787123
1213	36	-0.14580232346618516
1213	37	-0.3652766205300111
1213	38	-0.2801469094220863
1213	39	-0.22246333945620478
1213	40	-0.2365387589007426
1213	41	-0.29804549005777403
1213	42	-0.2831061385232161
1213	43	-0.07696807318923504
1213	44	-0.06566866599631282
1213	45	-0.163517750922189
1213	46	-0.23163747972556337
1213	47	-0.17203131560683005
1213	48	-0.24588381788089464
1213	49	-0.05414294917518703
1213	50	-0.21355380360771647
1213	51	-0.49484559110168647
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	26.0
14	65.0
15	165.0
16	408.0
17	748.0
18	1639.0
19	4242.0
20	10352.0
21	25704.0
22	57912.0
23	115147.0
24	207584.0
25	337200.0
26	503862.0
27	700769.0
28	925384.0
29	1163419.0
30	1415845.0
31	1681901.0
32	1961788.0
33	2284427.0
34	2674994.0
35	3204696.0
36	4001369.0
37	5366663.0
38	7945522.0
39	7904565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.15385788315993	24.098693761121844	15.454773346589965	29.29267500912826
2	16.007261613930122	22.045518369135713	36.52218221720657	25.42503779972759
3	18.854391793600566	24.30622352069573	30.05514867660778	26.784236009095924
4	14.644363190788765	19.308331864184993	35.90552969864936	30.141775246376884
5	15.209146358294113	35.99076853329821	32.914787183447515	15.885297924960165
6	29.99362460058451	37.50226285146086	18.04188147724685	14.462231070707777
7	25.812534177897483	32.26604128345281	21.809877199707074	20.11154733894264
8	23.88499429728125	35.409959578455556	21.307281650253785	19.397764474009406
9	23.37200492549992	16.806478911612203	24.861204115727737	34.960312047160144
10	17.354833634484354	29.047118982816357	31.140491745688465	22.457555637010827
11	33.280560231965076	24.257183497226837	23.260651955976407	19.201604314831677
12	20.33649111476439	26.97253840131435	29.2518469271642	23.439123556757057
13	27.50165149294892	22.040129660874545	24.984920486317936	25.473298359858603
14	20.816800068956436	22.90064967154745	26.032606952537645	30.24994330695847
15	23.52640154411208	29.300556938980026	23.107857826158767	24.065183690749123
16	23.027380415332622	29.800046342752307	24.376012394650775	22.7965608472643
17	21.374026132144802	28.407224734840465	27.933048065227737	22.285701067786995
18	21.651494360804282	28.050152110542452	26.968410992583674	23.329942536069588
19	22.273572980787055	27.76596095899612	27.985805478991548	21.974660581225276
20	22.76297682365872	27.13502747068181	26.885891055658202	23.21610465000127
21	22.668665298848747	27.014518430821322	26.580349858836268	23.736466411493662
22	22.947463051985327	26.897988551829467	27.47062768638951	22.683920709795704
23	22.577549339065143	27.979155503465385	26.42776986481936	23.015525292650103
24	23.484268560461725	26.86254319395595	26.786442624771222	22.866745620811102
25	23.091039929808638	27.34104258297474	26.096235876184988	23.471681611031634
26	22.481026682948208	27.183967222063632	26.17895481995165	24.156051275036507
27	22.421061419980006	27.603912575344374	26.58708293098367	23.387943073691947
28	21.955931989211667	27.60445271138478	26.559088458052603	23.88052684135095
29	22.031264339686132	27.00082596592126	27.046828267330575	23.92108142706203
30	23.01069348806022	26.469702176033916	27.752879326461187	22.766725009444677
31	22.55782673182155	27.390673336340644	26.85260265093396	23.19889728090385
32	22.372362747063757	26.45495925454215	28.01605343740675	23.15662456098734
33	21.800920965340364	26.912106808822383	27.700634255043905	23.586337970793345
34	21.593374966461557	26.25371764562521	27.697523336178477	24.455384051734757
35	22.827605918260268	25.97155666445569	27.956109017214896	23.244728400069143
36	21.266401494078565	26.50075494645246	29.074173959618836	23.15866959985014
37	21.21368917924728	25.14667317318469	28.967452207285877	24.672185440282153
38	21.232035049106184	24.560207609500743	30.18625262248293	24.021504718910144
39	20.143036190498783	24.044770947515037	31.80846307933138	24.0037297826548
40	20.46865031139879	23.472632519307002	31.569641124977437	24.489076044316768
41	19.684253103949906	24.341135759925876	31.930470599630866	24.044140536493348
42	18.433923111605548	23.2262615376987	33.47214769722808	24.867667653467667
43	18.619318973529346	23.40439246369746	33.41604690128054	24.560241661492658
44	16.925097286680284	23.57899909058295	34.656842436701154	24.839061186035618
45	16.72739631162243	22.58630488929963	35.37973567684813	25.306563122229807
46	16.034447475676046	23.660554010654515	35.971446921620206	24.333551592049233
47	15.129014116749943	23.218039239200362	36.09108007837843	25.56186656567126
48	15.474966161202246	23.547755616647404	35.591870800389756	25.385407421760593
49	13.854271906429853	24.140250456556036	36.50207053963742	25.50340709737669
50	13.082921950331128	25.070808973475266	34.6943837898487	27.1518852863449
51	13.5266020608879	25.082582520219322	34.90392001030324	26.486895408589543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	147.5
2	210.0
3	340.0
4	470.0
5	685.0
6	900.0
7	1958.0
8	3016.0
9	4119.0
10	5222.0
11	8588.0
12	11954.0
13	17715.0
14	23476.0
15	32852.0
16	42228.0
17	66107.5
18	89987.0
19	116708.5
20	143430.0
21	189601.5
22	235773.0
23	290861.0
24	345949.0
25	443209.0
26	657134.5
27	773800.0
28	914275.0
29	1054750.0
30	1231732.0
31	1408714.0
32	1597996.5
33	1787279.0
34	1969373.0
35	2151467.0
36	2329945.0
37	2508423.0
38	2639397.0
39	2770371.0
40	2847516.5
41	2924662.0
42	2923045.5
43	2921429.0
44	2929450.5
45	2937472.0
46	2876712.0
47	2815952.0
48	2886101.0
49	2956250.0
50	2948437.5
51	2940625.0
52	2767534.0
53	2594443.0
54	2381631.0
55	2168819.0
56	2007748.5
57	1846678.0
58	1660877.5
59	1475077.0
60	1275353.0
61	1075629.0
62	897911.5
63	720194.0
64	597968.0
65	475742.0
66	389862.5
67	303983.0
68	245205.0
69	186427.0
70	147876.0
71	109325.0
72	86621.5
73	63918.0
74	49895.0
75	28253.5
76	20635.0
77	16262.5
78	11890.0
79	9498.0
80	7106.0
81	5155.5
82	3205.0
83	2386.5
84	1568.0
85	1203.0
86	838.0
87	629.5
88	421.0
89	283.0
90	145.0
91	112.5
92	80.0
93	49.0
94	18.0
95	17.0
96	16.0
97	9.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07556405311254975
2	1.3883529937843908E-4
3	2.353140667431171E-6
4	2.847300207591717E-4
5	1.8825125339449367E-5
6	5.553411975137563E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.4118844004587024E-5
27	4.5415614881421593E-4
28	0.001157745208376136
29	0.001640139045199526
30	0.0036779588631949195
31	0.005096902685655916
32	0.007805367593869193
33	0.009476097467745324
34	0.009680820705811836
35	0.009040766444270557
36	0.007805367593869193
37	0.008525428638103131
38	0.011059761136926503
39	0.011560980099089341
40	0.0073794491330641514
41	0.0073441520230526835
42	0.007520637573110021
43	0.008400712182729279
44	0.005040427309637567
45	0.004821585227566469
46	0.005847554558566459
47	0.004835704071571056
48	0.0053533950184059135
49	0.00508513698231876
50	0.005026308465632981
51	0.002409616043449519
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.2496397E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.973225224500046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.16751717287482	10.659777064216803
2	6.676763970255514	1.7323832551390381
3	1.5253773586521016	0.5936719207844009
4	0.5370476274696139	0.27868959309786795
5	0.5230888590948134	0.33930747907318914
6	0.42038038299031333	0.3272213633096953
7	0.2936277190978673	0.2666508972409005
8	0.28030842231446995	0.29092034360079144
9	0.23880460599806064	0.27882593444347514
>10	3.7159820637129326	11.880580438795354
>50	1.4788297649939008	13.983755712244145
>100	2.083963650231814	53.057541786696646
>500	0.05613647238247037	4.30094770458213
>1k	0.0018769004234299685	0.37416630089044184
>5k	1.0735250785635167E-4	0.0815452494702693
>10k+	1.8767699996164475E-4	1.5540149564149373
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTAT	224719	0.5287954176444652	No Hit
CTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCG	176819	0.4160799796745122	No Hit
CTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCG	57040	0.13422314367027396	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGTC	47968	0.11287545153533839	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.353140667431171E-6	0.0	1.1168240921695078
2	2.353140667431171E-6	0.0	1.1204902853293657
3	2.353140667431171E-6	0.0	1.123165806268235
4	2.353140667431171E-6	0.0	1.4226641378562046
5	2.353140667431171E-6	0.0	1.4748450321564908
6	2.353140667431171E-6	0.0	1.4775676159087088
7	2.353140667431171E-6	0.0	1.488135570646142
8	2.353140667431171E-6	0.0	1.4926088910549287
9	2.353140667431171E-6	0.0	1.5800210074279943
10	9.177248602981565E-5	0.0	1.6152451700787716
11	9.177248602981565E-5	0.0	1.6355739523047095
12	9.177248602981565E-5	1.0824447070183385E-4	1.7218377360320687
13	9.177248602981565E-5	1.0824447070183385E-4	1.7510613899808964
14	9.177248602981565E-5	1.0824447070183385E-4	1.858872882799923
15	9.177248602981565E-5	1.0824447070183385E-4	1.868280739188313
16	9.177248602981565E-5	1.0824447070183385E-4	1.8720669425222096
17	9.177248602981565E-5	1.1059761136926503E-4	1.876653213683033
18	9.177248602981565E-5	1.3177587737614555E-4	1.8779521473314549
19	9.177248602981565E-5	1.3177587737614555E-4	1.8802582251855375
20	9.177248602981565E-5	1.3412901804357673E-4	1.8848492026276957
21	9.177248602981565E-5	1.3883529937843908E-4	1.891830970987964
22	9.647876736467799E-5	1.3883529937843908E-4	1.9001799140760098
23	9.647876736467799E-5	1.3883529937843908E-4	1.9102419435699454
24	9.647876736467799E-5	1.3883529937843908E-4	1.9173437221042526
25	9.647876736467799E-5	1.3883529937843908E-4	1.9339263043876402
26	9.647876736467799E-5	1.4118844004587023E-4	1.9489464012678535
27	9.647876736467799E-5	1.4118844004587023E-4	2.053658807827873
28	9.647876736467799E-5	1.4118844004587023E-4	2.446781547150927
29	9.647876736467799E-5	1.4118844004587023E-4	2.9427977152980755
30	9.647876736467799E-5	1.435415807133014E-4	3.4046556935167938
31	9.647876736467799E-5	1.435415807133014E-4	3.8781993682899754
32	9.647876736467799E-5	1.435415807133014E-4	4.468774141017178
33	9.647876736467799E-5	1.435415807133014E-4	5.342069352373567
34	9.647876736467799E-5	1.435415807133014E-4	6.485067428186912
35	9.647876736467799E-5	1.4589472138073258E-4	7.894761054684236
36	9.647876736467799E-5	1.4589472138073258E-4	9.78953109836582
37	9.647876736467799E-5	1.4589472138073258E-4	12.320060921870624
38	9.647876736467799E-5	1.4589472138073258E-4	15.263133954626788
39	1.0353818936697151E-4	1.4824786204816376E-4	18.1671589711476
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	43840	0.0	28.8179	45
TCTCGTA	40275	0.0	28.413582	44
ATCTCGT	42875	0.0	26.701445	43
TTATACC	33960	0.0	25.48619	8
TATCTCG	49845	0.0	22.841484	42
TATACCT	39570	0.0	21.810366	9
TATGCCG	13675	0.0	21.274677	43
TGCCGTC	13655	0.0	21.25598	45
ATGCCGT	14315	0.0	20.11888	44
CGTGATA	64070	0.0	20.106268	37
CGTATGC	15000	0.0	19.770561	41
TGATATC	58570	0.0	19.543085	39
ATATCTC	58615	0.0	19.354813	41
GATATCT	59065	0.0	19.314198	40
GTGATAT	61565	0.0	19.042088	38
GTCGTGA	68100	0.0	19.04194	35
TCGTGAT	69920	0.0	18.475527	36
CGTCGTG	72905	0.0	17.956703	34
ACGTCGT	73020	0.0	17.85757	33
GTATGCC	16760	0.0	17.546762	42
>>END_MODULE