FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n01 dn_rfp_3_march.341000000030b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n01 dn_rfp_3_march.341000000030b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42496397
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTAT2247190.5287954176444652No Hit
CTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCG1768190.4160799796745122No Hit
CTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCG570400.13422314367027396No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGTC479680.11287545153533839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT438400.028.817945
TCTCGTA402750.028.41358244
ATCTCGT428750.026.70144543
TTATACC339600.025.486198
TATCTCG498450.022.84148442
TATACCT395700.021.8103669
TATGCCG136750.021.27467743
TGCCGTC136550.021.2559845
ATGCCGT143150.020.1188844
CGTGATA640700.020.10626837
CGTATGC150000.019.77056141
TGATATC585700.019.54308539
ATATCTC586150.019.35481341
GATATCT590650.019.31419840
GTGATAT615650.019.04208838
GTCGTGA681000.019.0419435
TCGTGAT699200.018.47552736
CGTCGTG729050.017.95670334
ACGTCGT730200.017.8575733
GTATGCC167600.017.54676242
Produced by FastQC (version 0.11.2)