FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l02n01 dn_rfp_26_feb.34100000003014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l02n01 dn_rfp_26_feb.34100000003014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6708917
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC952801.4201994151962232RNA PCR Primer, Index 39 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG241370.3597749085284555No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT106800.15919111832804014No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT75010.11180642121522742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC143650.038.85956245
ATGCCGT163900.034.23688544
TATGCCG197400.028.80323843
GTATGCC201850.028.17959842
CGTATGC207600.027.48600641
TCGTATG211000.027.0646340
TCTCGTA194950.025.48463838
CTCGTAT216650.025.44487439
CATCTCG190950.025.19362636
ATCTCGT199050.024.8240637
CTCTCTA227950.024.17382429
CCTCTCT231250.023.867628
CTACATC202500.023.79016933
TCTCTAC232100.023.78036930
CTCTACA232500.023.69106931
ACCTCTC232150.023.6002627
AGACCTC235700.023.32047525
GAGACCT236900.023.30647324
GACCTCT235850.023.2867426
TCTACAT214050.023.22129232
Produced by FastQC (version 0.11.2)