FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 ycs4-1rt wt.342000000089c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 ycs4-1rt wt.342000000089c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16683957
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190180.11398974475899212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG17350.027.0998651
CGGGGAC16600.026.8335061
CGGGGTT26800.021.2376291
CGGGGTG15050.020.1798341
CGGGGAT33450.020.1764811
CGGGGGG6150.020.119071
GGGGGAT35650.019.5624161
CGGGGGT12750.017.9974231
GGGGTTA47550.017.7430572
TGGGGTT69700.017.6875671
GCGGCGC11900.017.5815141
CGGGGCG6850.017.4062651
GAGGGAT48050.016.8082071
GGGGACC23400.016.1525522
CGGGGGA13350.015.8404281
CGGGGAA62550.015.7891061
GGGGTAA51050.015.7333132
GGGGACA34900.015.0202872
GGGGGCG18700.014.9176491
GGGGAAC42650.014.8757142
Produced by FastQC (version 0.11.2)