FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 ycs4-1 double rt.342000000089fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 ycs4-1 double rt.342000000089fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9630600
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110220.11444769796274375No Hit
GCGGCGCTACCATGAGAAATGGGTGAATGTTGAGATAATTGTTGGGATTCC97980.10173820945735468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGC27450.035.976341
CGGCGCT43050.022.9418892
CGGGGAG10200.022.4954591
GCGGGGC8950.022.118441
CGGCGTT14200.021.7033651
CGCTACC55600.020.7582955
GCGCTAC56000.020.4090374
GGGGCGC9750.019.6114251
GGCGCTA59200.019.6098773
CGGGGGT7950.018.1095521
CTCGGTT13550.018.10021817
CGGGGCT15050.017.9365791
GGGGATC18800.017.7109432
GCGGGCT9200.017.3606241
CGGGGAC11450.017.2890851
GGGGGAT19150.016.7981741
CGGGGAT18450.016.5820181
CCATGAG88650.016.46884210
CGGGCTA8650.016.3855952
CTACCAT95950.016.272857
Produced by FastQC (version 0.11.2)