FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 ycs4-1 double 37.34200000008a0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 ycs4-1 double 37.34200000008a0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41657697
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN475760.11420698556619681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC64150.021.78188144
CGGGGAG48600.020.5979181
GTCGCCG73350.019.1110145
CGGGGAC53900.017.9882141
CTCGGTG131650.017.15984244
CGGGGTG36200.017.0271341
CGGGGCT40450.015.8498781
CGGCGCT71200.015.7975441
CGGGGGT30000.015.5220351
CGGGGAT82350.015.3249421
GGGGTTA95900.015.2018232
CGGGGTT62700.015.104771
GGGGGAT77600.014.9005051
TGGTCGC96200.014.71175643
AGGGGTG75700.014.5910041
GGCGGTA104050.014.5719391
AGGGGTT99700.014.4631351
GCACACG48350.014.38082718
TCTCGGT159150.014.37815943
TGGGGTT140550.014.3249051
Produced by FastQC (version 0.11.2)