FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 ycs4-1 37 wt.342000000089ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 ycs4-1 37 wt.342000000089ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326776
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60580.11372732774946796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT8600.017.5248221
CGGGGTG3900.017.30351
CGGGGCG2105.456968E-1217.1387041
CGGGGAG5450.016.509761
GCGGGTG7950.016.4111161
GGGGGAT10250.016.2399651
CGGGGAC4850.015.7693731
GGCGGAT11650.015.2538141
GGGCGAC6950.014.8884781
CGCGGAA5300.014.854891
GGGGATT12700.014.7030982
CGGGGAA13550.014.6090051
CGGGGGT3700.014.5910581
CGTGGAT11500.014.4747521
GGGGGGC6850.014.4490521
CAGGGAT10750.014.438361
CGGGGTT10000.014.396511
CGGCGTT6150.014.2648341
GGGCGTA6150.014.2648341
GGCGGTA14200.014.2571071
Produced by FastQC (version 0.11.2)