FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 ycs4-1 30 wt.342000000089d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 ycs4-1 30 wt.342000000089d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11893895
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135080.11357086976133554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCG4400.024.0294551
CGGGGAC10150.022.3848441
CGGGGAG11950.022.025041
CGGGGTG8350.018.8586371
CGGGGGG4300.018.8335761
CCGGGTG7900.016.8005561
CGGGGGA8450.016.2394711
CGGGGAT19400.015.538241
GGGGACC13000.015.4024322
GCGGGTC6900.015.3231311
GCGGGTG15500.015.2389961
CGGGCTA7150.015.1019821
GGGGGAT20050.015.0345061
CGGGGTT16200.014.8582451
TGGGGTT39700.014.5626821
CGCGGTT7650.014.4089851
GGGGTTA26450.014.2898042
CGGGGGT8450.014.1097041
GGGGACA22750.013.9437762
CGCGGAT8250.013.9064081
Produced by FastQC (version 0.11.2)