FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 maf1d wt.342000000089ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 maf1d wt.342000000089ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14367613
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG382630.26631424440510754Illumina Single End PCR Primer 1 (100% over 51bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN164600.11456321937401849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG51750.034.5677245
GGTCGCC51650.034.50419244
CTCGGTG58550.031.16487938
GTGGTCG59750.030.46578842
TGGTCGC59850.030.2270143
TCGGTGG68350.026.72929439
GGTGGTC70850.025.819641
TCTCGGT73700.024.81993537
CGGTGGT77050.023.88798140
ACACTCT56550.020.6464611
ATCTCGG96550.018.92262336
CACTCTT67200.017.6102392
TAGATCG50000.016.5580229
GATCTCG120700.015.15538235
GTGACTG30100.014.7234861
TTAGATC60200.014.1271548
GACTGGA32650.013.9193833
AGATCTC140650.013.29367134
CGTGTAG137850.013.187407516
AGCGTCG139800.013.06665111
Produced by FastQC (version 0.11.2)