FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 maf1d rrm3d pif1m2.342000000089b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 maf1d rrm3d pif1m2.342000000089b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367889
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143950.116390113138952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC18150.026.77945544
GTCGCCG20250.024.11315545
CTCGGTG22900.022.10724638
GTGGTCG24700.020.40617442
TGGTCGC25850.019.49835643
TCTCGGT28500.017.60568637
TCGGTGG30200.016.91238239
GGTGGTC32850.015.68581741
ATCTCGG35750.014.34999436
CGGTGGT37350.013.795958540
GATCTCG45000.011.60041935
ACACTCT29550.010.5813931
CGTGTAG49700.010.41145816
AGCGTCG50200.010.39600111
GCGTCGT50000.010.30261512
GGGGGCG8300.010.0278811
CGTCGTG51250.09.96415313
GTAGGGA54250.09.7919
GTGTAGG52550.09.76232517
GTCGTGT51200.09.75490714
Produced by FastQC (version 0.11.2)