FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 maf1d rrm3d.34200000008a17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 maf1d rrm3d.34200000008a17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15919795
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG301500.18938686082327066Illumina Single End PCR Primer 1 (100% over 51bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182410.11458062117005903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC44150.032.97425544
GTCGCCG45950.031.63348245
CTCGGTG51250.029.28235438
GTGGTCG51150.028.94453642
TGGTCGC52850.027.75804943
TCGGTGG62900.024.03745839
TCTCGGT62500.023.94036337
GGTGGTC63950.023.57323641
CGGTGGT71850.021.04389440
ACACTCT55250.018.5666751
ATCTCGG83600.018.05948336
TAGATCG49050.015.364649
CACTCTT69750.014.3536092
GATCTCG106150.014.22319135
AGCGTCG115350.012.93005211
CGTCGTG117200.012.80317213
GCGTCGT116000.012.79945912
CGTGTAG117900.012.65174816
GTCGTGT118100.012.55412114
GAGCGTC121050.012.46987310
Produced by FastQC (version 0.11.2)