FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n02 maf1d pif1m2.34200000008a24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n02 maf1d pif1m2.34200000008a24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10025092
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG386920.3859515703197537Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG142760.1424026831873463Illumina Single End PCR Primer 1 (100% over 42bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG118740.1184428033179147Illumina Single End PCR Primer 1 (100% over 41bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113310.11302639417174426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC49450.035.99210744
GTCGCCG51700.034.55628645
GTGGTCG55150.032.71964642
TGGTCGC56550.031.55151743
CTCGGTG59000.030.73693738
GGTGGTC60850.029.9135341
TCGGTGG61700.029.35527239
ACACTCT56700.028.0907021
CGGTGGT65100.027.82240140
TCTCGGT69100.026.34261537
CACTCTT66200.023.7216322
GTGACTG31400.022.9262351
GACTGGA31750.022.8174953
TGACTGG32200.022.0090792
GCACACG16550.021.07447818
ATCTCGG105850.017.19673736
TTAGATC61450.017.0983878
GAGCACA26600.016.66284416
ACTCTTT88800.016.6206023
TCAGATC50200.015.4610389
Produced by FastQC (version 0.11.2)