FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 ycs4-1rt wt.341000000089c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 ycs4-1rt wt.341000000089c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16683957
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG32000.011.2498695
GTGCCCC33850.010.5020944
CCCTACG23050.09.95682940
CCCCGGG35650.09.9080687
CTACGGC24750.09.545629542
CCCGGGT37400.09.4444558
CGGCCTT24650.09.3104645
CCGGGTA45950.09.2539079
TACGGCC24000.09.187613543
GCCCCGG40600.08.8113426
CCCCCCC30250.08.70258743
CCTACGG27600.08.64141241
ACCCCCC29850.08.593122542
GACAGGT36850.08.55532551
CCCCGGA21750.08.3863261
CCCCCCT36400.08.2830744
ACGGCCT27600.08.23379844
CCCCCTC32400.08.12510145
CGGGTAA56000.07.954740510
GGTGCCC46100.07.7602143
Produced by FastQC (version 0.11.2)