FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 ycs4-1 double rt.341000000089ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 ycs4-1 double rt.341000000089ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9630600
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGG15300.012.7929447
TGCCCCG15500.012.6294495
CCCGGGT16200.012.4988538
GTGCCCC17100.011.4477454
CCCTACG11150.011.09913540
CCGGGTA22300.010.6940879
TAACTAG35600.010.61827130
TACGGCC11450.010.41491943
CGGGTAA29050.09.75814110
CTGCCGG17350.09.596807530
CTACGGC13000.09.51949542
TGCCGGA17300.09.49453231
TGTTGGA51700.09.3650511
CGGCCTT13200.09.20456145
GCCCCGG21500.09.1047616
GGGTAAC25600.09.05190411
TCCCTAC14300.08.968966539
ACGGCCT14050.08.8078944
GCCGAGA10500.08.79341
GGAATAG36200.08.7019435
Produced by FastQC (version 0.11.2)