FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 ycs4-1 double 37.34100000008a0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 ycs4-1 double 37.34100000008a0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41657697
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT111900.011.96395943
CCCTACG58500.011.50029840
CGTATGC138850.010.24131145
ATCTCGT128650.09.86408541
TACGGCC70100.09.85390543
TGTAATC210750.09.80083643
TAATCTC212150.09.67244245
GTAATCT213850.09.65873944
TCGTATG154100.09.62209744
CTACGGC70550.09.599730542
AATCTCG130500.09.48300440
CGGCCTT78200.09.17842845
TCTCGTA142700.09.0663742
TCCCTAC80200.08.75345939
CCTACGG77950.08.65949541
CCTTGTA340950.08.57245944
CGTGTGC57700.08.3916741
GTCCCTA82400.08.38322638
ACGGCCT83150.08.30736144
AGTCACC363350.07.963707439
Produced by FastQC (version 0.11.2)