FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 ycs4-1 37 wt.341000000089e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 ycs4-1 37 wt.341000000089e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326776
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC7051.3096724E-107.659497743
TCACGTG6753.3851393E-97.33339746
CACGTGA6305.482616E-87.14238367
CCCTACG7251.88993E-97.138060640
CCTACGG8401.1641532E-106.96441241
CGGCCTT8804.7293724E-116.903275545
CCCCCCC8052.3628672E-96.70800843
CCTCGCT5104.484811E-56.62323861
ACGGCCT8203.5161065E-96.585344
CTACGGC8304.5620254E-96.506020542
CCGGGTA8558.629286E-96.31537069
CCCCTGG3950.00579297126.27111671
ACCCCCC9003.921741E-96.24999642
CCCCGGG6201.5942744E-56.16894537
CCCCCGA4400.00261598486.14154861
GTCCCTA10801.6370905E-106.041889738
CCCACCG4700.0054560995.7495351
CCCGGGT6705.226605E-55.7085768
GTGCCCC7102.0631109E-55.70438244
CCCCCCT11102.0081643E-95.675618644
Produced by FastQC (version 0.11.2)