FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 ycs4-1 30 wt.341000000089d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 ycs4-1 30 wt.341000000089d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11893895
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG21300.011.40852455
GTGCCCC23150.010.4968294
CCCTACG13350.010.44963840
CCCCGGG24000.010.0303047
CCCGGGT24950.09.738568
CTACGGC14850.09.54555742
TACGGCC15400.09.35067143
CCGGGTA31950.09.2244829
GCCCCGG26350.09.1363366
CGGCCTT16900.08.92021545
CCTACGG16300.08.69637641
GGTGCCC29500.08.61873
TCACGTG12400.08.1650676
CGGGTAA41650.07.724361410
GACAGGT25700.07.7114961
CGCGGGA10900.07.637632443
CACGTGA14550.07.57660447
GCGCGGG11100.07.500080642
CCCCGGA15050.07.48208431
CGCGCGG10850.07.465486541
Produced by FastQC (version 0.11.2)