FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 maf1d wt.341000000089af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 maf1d wt.341000000089af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14367613
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC291930.20318615207689686TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG54100.026.16070440
CGTATGC69950.025.60408845
CGATCTC56150.024.84537339
CTCGTAT66900.023.6101143
CACGATC62550.022.30324237
TCGTATG83150.021.78307244
TCTCGTA66300.021.58408242
ATCTCGT67100.021.15893741
ACGATCT70650.019.96912238
TCACGAT99600.015.65593536
CATCACG129350.013.82943234
TAGATCG64150.013.747789
ATCACGA131200.013.61727835
GCACACG140300.013.10109312
GTAGATC65550.012.9050098
GTCACAT143350.012.85539130
CACACGT143800.012.76657613
ACACGTC144500.012.70473114
ACGTCTG147650.012.54034616
AGATCGG149600.012.4506891
Produced by FastQC (version 0.11.2)