FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 maf1d rrm3d pif1m2.341000000089bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 maf1d rrm3d pif1m2.341000000089bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367889
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32200.015.86198345
GATCTCG27450.015.24632440
CTCGTAT35750.013.27998243
TCGTATG41750.012.66491844
TCTCGTA38550.011.14812342
AGTGATC38850.011.12026937
ATCTCGT40150.010.59169641
GTGATCT40650.010.57250538
GTCACAC48850.010.54788730
GCACACG50200.010.53196712
CCCCGGG7800.010.0952917
CACACAG51550.010.08276432
CACACGT53450.09.84948513
ACACGTC53600.09.73797214
ACGTCTG57300.09.58033216
TAGATCG29700.09.3931369
TGATCTC44400.09.37548639
AGCACAC57600.09.25702111
CACGTCT58900.09.20549415
CGTCTGA58700.09.16020517
Produced by FastQC (version 0.11.2)