FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 maf1d rrm3d.34100000008a1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 maf1d rrm3d.34100000008a1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15919795
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC250480.15733870944946216TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC61850.023.8280245
TATCTCG53050.023.66715840
CTCGTAT66750.021.10157643
TCGTATG71750.021.07342344
TCTCGTA64200.019.97706442
ATCTCGT63950.019.87942541
GTATCTC69100.018.33292439
ATGTATC83750.015.287230537
TGTATCT94600.013.62902738
TAGATCG61100.013.40306959
CCGATGT114950.012.919134
CGATGTA117500.012.75362635
GCACACG119400.012.69988112
CACACGT121650.012.50197613
GTCACCG121700.012.38736230
ACACGTC123550.012.38255514
AGTCACC123100.012.20980929
CACCGAT125100.012.17659632
ACCGATG126050.012.08493933
CGTCTGA126550.012.017917
Produced by FastQC (version 0.11.2)