FastQCFastQC Report
Thu 23 Apr 2015
H3NH5BCXX l01n01 maf1d pif1m2.34100000008a27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NH5BCXX l01n01 maf1d pif1m2.34100000008a27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10025092
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC309030.30825652273315796TruSeq Adapter, Index 8 (100% over 50bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT119630.11933057571940488TruSeq Adapter, Index 8 (100% over 41bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA113450.11316604376298992TruSeq Adapter, Index 8 (100% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63800.028.56615845
CTCGTAT63650.026.93640943
ATCTCGT58500.026.57722541
TCTCGTA60950.025.4714842
TCGTATG72600.025.38226744
AATCTCG62100.025.18157440
GAATCTC74150.020.99874139
TGAATCT88550.017.8126238
TTGAATC101650.015.58352837
GTAGATC76900.014.0432538
GAGTGTA52700.013.6194294
GTGTAGA69950.013.0591526
GTCACAG37050.012.8136555
AGTGTAG64050.012.6813915
TAGATCG91200.012.6800549
AGAGTGT58350.012.4934673
GTATGCC18800.012.32726945
ACAGATC48300.011.831498
CACAGAT49500.011.4537627
TGTAGAT93050.011.2915447
Produced by FastQC (version 0.11.2)