FastQCFastQC Report
Tue 14 Jun 2022
H3NGLBGXM_n02_GDO_A1_PS_ASV1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGLBGXM_n02_GDO_A1_PS_ASV1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72434595
Sequences flagged as poor quality0
Sequence length57
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6739170.9303800207621787No Hit
GCATAGCTCTTAAACACTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3743100.5167558402169571No Hit
GCATAGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAG2341560.3232654231034218No Hit
GCATAGCTCTTAAACAATGAAATTGGGGCAGTGGTATCAACGCAGAGTACTTTTTTT1793530.2476068237835802Clontech SMART CDS Primer II A (100% over 24bp)
GCATAGCTCAACTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG1283850.1772426559436137Illumina Single End PCR Primer 1 (100% over 42bp)
GCATAGCTCTAGTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG977660.1349714180082045Illumina Single End PCR Primer 1 (100% over 42bp)
GCATAGCTCTCCCCGGTCAGGAAAAGTTTGTTGTTCTTGTACTCCGGAAAGAGGCGG829220.11447844776380678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)