FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l02n02_smallrna_salt24h_rep2_root.3520000006c1d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l02n02_smallrna_salt24h_rep2_root.3520000006c1d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28644291
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT414249914.461866066086257Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT3392131.1842255058782918Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA1876790.6552056044954997No Hit
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1292380.4511824014076662Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1183630.41321672091656936Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA1077830.37628091405718506Illumina RNA PCR Primer (97% over 45bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG1000970.34944834207975334Illumina RNA PCR Primer (100% over 34bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA977960.34141532775239575No Hit
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC839330.29301824925602105Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC765230.26714922006622543No Hit
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG706560.24666695363484473Illumina RNA PCR Primer (100% over 34bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC665460.2323185447320026Illumina RNA PCR Primer (100% over 45bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA644610.22503960736888198Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTA566800.1978753811710683No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG561750.19611237715745872Illumina RNA PCR Primer (100% over 34bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT540380.18865190274739216Illumina RNA PCR Primer (100% over 38bp)
GGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCG488120.17040742952932575No Hit
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG450110.15713776961698928Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG444710.1552525772063969Illumina RNA PCR Primer (100% over 36bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG441610.1541703371188346Illumina RNA PCR Primer (100% over 33bp)
CGGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG435000.15186272196438727Illumina RNA PCR Primer (100% over 37bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT405150.1414417972502793Illumina RNA PCR Primer (100% over 35bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT398090.13897708272828257Illumina RNA PCR Primer (100% over 35bp)
CGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT398060.13896660943711261Illumina RNA PCR Primer (100% over 31bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT390880.13646000175043607No Hit
GACTACAATTCGGACGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG387280.13520320681004114Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT386810.13503912524837847Illumina RNA PCR Primer (100% over 38bp)
AGCCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC380440.13281529642329076Illumina RNA PCR Primer (100% over 39bp)
TCCAGACTACAATTCGGACGGCGGATCGTCGGACTGTAGAACTCTGAACGT372450.13002590987502535Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCCAGGAGCGCATCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTG369010.12882497248753688Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC365620.12764149058533164Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG358880.1252884911691478Illumina RNA PCR Primer (100% over 36bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT358120.1250231677928422Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG355400.12407358939343271Illumina RNA PCR Primer (100% over 37bp)
GGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCAC345440.12059645672500674No Hit
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT335140.11700062675665458Illumina RNA PCR Primer (100% over 38bp)
AGGCCAGGAGCGCATCGCCGGCAGAAGGGTCGAGCAGGTCGGTGCTCGCCG329480.11502466582258922No Hit
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC316540.11050718623128078Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT313310.10937956188198199Illumina RNA PCR Primer (97% over 46bp)
GGAGCCCGCGTCAGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC310870.10852773420015877Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC309590.10808087377690723Illumina RNA PCR Primer (100% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT4458150.043.79091345
ATAACCT131000.043.15464810
GATAACC133450.042.5983779
CGATAAC134050.042.5070658
GGGAATG395700.042.4557462
GGAATAC223350.041.9808466
CGCCGTA4806500.041.86671444
CACTATA223200.041.86101517
ACGATAA137250.041.8290987
TAACCTA132100.041.8246111
CCGTATC137500.041.82398245
ACTATAC224250.041.6951118
TATACTA225400.041.6420820
GGTCGCC4833100.041.62856341
TCGCCGT4844550.041.5113443
TGGTCGC4878700.041.31136340
AACGACG224750.041.23759528
GACGATA140700.041.2190746
GGGAATA228000.041.085975
GCGGGAA228000.041.016873
Produced by FastQC (version 0.11.2)