FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l02n02_smallrna_salt24h_rep1_root.3520000006c1bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l02n02_smallrna_salt24h_rep1_root.3520000006c1bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16105241
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT354405522.00560053711708Illumina RNA PCR Primer (100% over 46bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA4225932.623947074123262No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA1074190.6669816365989184Illumina RNA PCR Primer (97% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC990010.6147129372357731Illumina RNA PCR Primer (100% over 45bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA960620.5964642193184193No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA919340.5708328115052733Illumina RNA PCR Primer (100% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT753150.46764280025365657Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTA535870.33273019633794987No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG444490.27599090258879083Illumina RNA PCR Primer (100% over 34bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC425580.264249383166635No Hit
AGGCCAGGAGCGCATCGCCGGCAGAAGGGTCGAGCAGGTCGGTGCTCGCCG400320.24856504786236977No Hit
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG357150.2217601090228951Illumina RNA PCR Primer (100% over 34bp)
CGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT337450.20952806605005164Illumina RNA PCR Primer (100% over 31bp)
GGCCGCCCGATTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT333940.2073486512868699Illumina RNA PCR Primer (100% over 31bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG324480.2014747869963573Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT316500.1965198782185253Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACTACAATTCGGACGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG315350.19580582494853693Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG299630.18604502720574007Illumina RNA PCR Primer (100% over 33bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT265860.16507669770356123Illumina RNA PCR Primer (97% over 46bp)
GGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCAC256300.15914074182435395No Hit
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT243440.15115576351822366Illumina RNA PCR Primer (100% over 35bp)
GGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCG242850.1507894231449253No Hit
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC230300.1429969287637484Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGCCCGCGTCAGCCGATCGCGGACTGTAGAACTCTGAACGTGTAGATCT228920.1421400648397624Illumina RNA PCR Primer (96% over 31bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC225440.13997927755318906Illumina RNA PCR Primer (97% over 47bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC223460.13874986409703524Illumina RNA PCR Primer (97% over 45bp)
GGCCGCCCGATTCTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT220760.13707339120228004Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG208130.12923122355014743Illumina RNA PCR Primer (100% over 37bp)
GGCGACAGGGTCCGGGCCGGACGGGGCGATCGTCGGACTGTAGAACTCTGA205070.12733122093609156Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACGATCGTCGGACTGTAGAA201880.12535049925673264No Hit
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC197330.12252533197112667Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG197090.12237631215825955Illumina RNA PCR Primer (100% over 34bp)
ACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGAA189500.1176635605763366No Hit
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT185210.11499983142133668Illumina RNA PCR Primer (97% over 46bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA182390.11324884862014793Illumina RNA PCR Primer (100% over 40bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG180760.11223675572442536Illumina RNA PCR Primer (100% over 33bp)
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT173620.10780341629162829Illumina RNA PCR Primer (100% over 35bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT171490.10648086545243253Illumina RNA PCR Primer (100% over 31bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC162080.10063804695626723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC78350.043.5827758
ACTATAC486100.043.5431518
CACTATA485750.043.54210717
GGAATAC488550.043.522286
GGGAATA487950.043.482285
TATACTA487750.043.4788820
CTATACT488550.043.4030819
TACTACA488450.043.3492622
ATACTAC489200.043.3301321
CCGTATC182950.043.28626645
AGGCGGG487100.043.2658651
GCGGGAA490250.043.251013
ACAACGA488950.043.22876426
AACGACG489000.043.14613728
ATAACCT78200.043.06376310
GCCGTAT3869200.043.0252345
CAACGAC492350.042.88455227
ACGATAA79150.042.8596577
ATACTCA493600.042.835469
AATACTC496400.042.8280338
Produced by FastQC (version 0.11.2)