FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l02n02_smallrna_heat_1h_rep3_root.3520000006c21b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l02n02_smallrna_heat_1h_rep3_root.3520000006c21b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24816909
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1451011958.46867956037555Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4631261.866171165796675Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA3979591.6035800429457192Illumina RNA PCR Primer (97% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC3157791.2724348548000075Illumina RNA PCR Primer (100% over 45bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1158600.46685910803799135Illumina RNA PCR Primer (97% over 46bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA749530.30202391442060733Illumina RNA PCR Primer (100% over 40bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC741390.2987438927224982Illumina RNA PCR Primer (97% over 45bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC727730.293239581125917Illumina RNA PCR Primer (97% over 47bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT602580.2428102548951604Illumina RNA PCR Primer (97% over 46bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT288060.11607408481048144Illumina RNA PCR Primer (97% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT258520.10417091024510748Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA249280.10044764237157819No Hit
GATCGTCGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA248200.10001245521752931Illumina RNA PCR Primer (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC572500.044.21366545
GCCGTAT15687400.043.56686445
CGATAAC38800.042.7857138
ATAACCT38100.042.62887210
GCCGTAA478600.042.2964445
ACGATAA39100.042.2868847
AGCCTGA190350.042.17631
GGAATAC30750.041.9914636
GCAGTAT29400.041.83689545
TAACCTA38100.041.80243711
GATAACC39750.041.7648439
CGCCGTA16973100.041.5173744
TCGCCGT16978950.041.46539743
GGTCGCC17019600.041.41292641
TGGTCGC17060850.041.39081640
GTCGCCG17048150.041.376242
GTGGTCG17089900.041.32182339
CCACGGG94100.041.305991
GGTGGTC17142000.041.18520738
CGGTGGT17213000.041.0230837
Produced by FastQC (version 0.11.2)