FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l02n02_smallrna_heat_1h_rep2_root.3520000006c1f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l02n02_smallrna_heat_1h_rep2_root.3520000006c1f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20318945
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT851423241.90292360159447Illumina RNA PCR Primer (100% over 46bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA2712561.3349905716069412Illumina RNA PCR Primer (97% over 45bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2245701.1052247053181157Illumina RNA PCR Primer (100% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT1543500.7596358964503325Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC1377890.6781306805053117Illumina RNA PCR Primer (100% over 45bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT566250.27868080749271185Illumina RNA PCR Primer (97% over 46bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC420010.2067085668079716Illumina RNA PCR Primer (97% over 47bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC397480.1956203926926324Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA350360.17243021229694752Illumina RNA PCR Primer (100% over 40bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT312250.15367431724432543Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT304620.14991920102150974Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG288630.1420496979542983Illumina RNA PCR Primer (100% over 34bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT285780.14064706607552704Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGTGGGTTTCCCCATTCAGAAATCTCCGGATCAAAGCTTATTTGCCAGCT282120.1388457914522629No Hit
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT250760.12341191927041488Illumina RNA PCR Primer (97% over 46bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG227430.11193002392594693Illumina RNA PCR Primer (100% over 34bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT214140.10538933000704516Illumina RNA PCR Primer (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC273500.044.10006345
GCCGTAT9060400.043.85234545
GGGAATG176650.043.2485662
GCCGTAA311750.043.15643745
CGATAAC36800.042.9722488
GATAACC36600.042.8396729
ACGATAA37050.042.623147
ATAACCT37100.042.38358710
CGCCGTA9795750.041.99028844
TCGCCGT9812800.041.88981643
GGTCGCC9822750.041.86909541
GTCGCCG9849800.041.79576542
TGGTCGC9866100.041.764940
AGCCTGA157000.041.74191
CCTGACG159700.041.6502383
GTGGTCG9911700.041.5820639
GGTGGTC9998400.041.21563338
GGGAATA23900.041.0626645
GTATAGG11700.040.9559521
ACTATAC24700.040.8965418
Produced by FastQC (version 0.11.2)