H3NGHBCXY_l02n02_smallrna_drought_1h_rep1_root.3520000006c242.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l02n02_smallrna_drought_1h_rep1_root.3520000006c242.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7910674
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	3282956	41.50033233577822	Illumina RNA PCR Primer (100% over 46bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	91032	1.1507489753717572	Illumina RNA PCR Primer (97% over 45bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	73141	0.9245861983441614	Illumina RNA PCR Primer (100% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC	45453	0.5745780953683592	Illumina RNA PCR Primer (100% over 45bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	31070	0.39276046516390384	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	21347	0.2698505841600855	Illumina RNA PCR Primer (97% over 46bp)
TTCCACACCCAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGATCG	19028	0.24053576218663544	No Hit
GGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTACC	18806	0.23772942735347202	No Hit
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	17015	0.2150891314697079	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC	15245	0.19271429969178352	Illumina RNA PCR Primer (97% over 47bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	14764	0.18663390755326284	Illumina RNA PCR Primer (100% over 40bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	12997	0.16429699921902988	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC	12927	0.1634121188662306	Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAN	12652	0.15993580319451922	Illumina RNA PCR Primer (97% over 45bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	12459	0.15749606165037267	Illumina RNA PCR Primer (100% over 34bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	9698	0.12259385230639008	Illumina RNA PCR Primer (100% over 32bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	8782	0.1110145608326168	Illumina RNA PCR Primer (97% over 46bp)
TTCCACACCCAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGATCGT	8061	0.10190029319878433	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	348935	43.978878	45
CCGTATC	9660	43.086014	45
GCCGTAA	10620	42.78891	45
CGCCGTA	374545	42.260838	44
TCGCCGT	374795	42.190964	43
GGTCGCC	375840	42.1115	41
TGGTCGC	377175	42.032627	40
GTCGCCG	376960	42.01045	42
GTGGTCG	378825	41.86499	39
GGTGGTC	381815	41.531845	38
ACGTGGG	1020	41.46131	9
CGGTGGT	385645	41.123386	37
TCGGTGG	389240	40.73317	36
AACGTAA	410	40.605038	16
ACGATGT	3835	40.473343	7
CTCGGTG	392805	40.36266	35
TCACACG	4340	39.913456	21
TCTCGGT	397265	39.863083	34
CCACGGG	3670	39.82247	1
ATAACCT	1035	39.773712	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content