FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l02n01_smallrna_salt24h_rep2_root.3510000006c1d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l02n01_smallrna_salt24h_rep2_root.3510000006c1d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28644291
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC414926414.485483337674513RNA PCR Primer, Index 9 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3273621.142852514659902RNA PCR Primer, Index 1 (100% over 30bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA1751200.6113609165609999No Hit
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1597790.5578039966148927RNA PCR Primer, Index 6 (100% over 34bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG1034240.36106322198723645RNA PCR Primer, Index 9 (100% over 39bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC934550.3262604754294669No Hit
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA817100.28525754049908236RNA PCR Primer, Index 7 (97% over 35bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC745470.2602508122822799RNA PCR Primer, Index 1 (100% over 33bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA723450.25256341656353093No Hit
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG649650.2267991202854349RNA PCR Primer, Index 9 (100% over 39bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTTGGAATTCTCGGGTG556670.1943388998526792No Hit
CGCCGTCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCC552080.19273648630367565No Hit
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG542980.18955958798212183RNA PCR Primer, Index 9 (100% over 39bp)
GGCTGACGCGGGCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA500400.17469449671489512RNA PCR Primer, Index 7 (97% over 35bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA493990.17245670350158082RNA PCR Primer, Index 9 (100% over 38bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT489920.17103582699952322RNA PCR Primer, Index 9 (100% over 43bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA440690.15384915618962255RNA PCR Primer, Index 9 (100% over 40bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT431610.15067924006218203RNA PCR Primer, Index 9 (100% over 41bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA421370.14710435667616978RNA PCR Primer, Index 9 (100% over 40bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA419900.14659116540884184RNA PCR Primer, Index 7 (97% over 35bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG419750.14653879895299207RNA PCR Primer, Index 9 (100% over 39bp)
GCCCCCGACGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT417980.14592087477396457RNA PCR Primer, Index 9 (100% over 36bp)
CAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTC407760.1423529735820656No Hit
CTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC393370.13732928491754257RNA PCR Primer, Index 9 (100% over 42bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG386090.13478776626029948No Hit
CGCCGTCCGAATTGTAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA376480.13143282198885636RNA PCR Primer, Index 1 (100% over 32bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC374570.13076602245103572RNA PCR Primer, Index 9 (100% over 42bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT370650.12939751240482789RNA PCR Primer, Index 9 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACCAGATCTCGTATGCC367300.1282279948908493RNA PCR Primer, Index 9 (98% over 51bp)
GCCGGCGATGCGCTCCTGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG363780.12699912872690758RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGTCCGAATTGTAGTCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA362150.12643007990667318RNA PCR Primer, Index 1 (100% over 28bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT343820.12003089900182902RNA PCR Primer, Index 1 (100% over 30bp)
GCGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC339300.11845292313222207RNA PCR Primer, Index 9 (100% over 44bp)
CTCGGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT320180.11177794555990234RNA PCR Primer, Index 9 (100% over 41bp)
GGCGAGCACCGACCTGCTCGACCCTTCTGCCGGCGATGCGCTCCTGGCCTT316240.1104024533195812No Hit
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT311580.10877560209118109RNA PCR Primer, Index 9 (100% over 43bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA303800.1060595285811054RNA PCR Primer, Index 7 (97% over 35bp)
CAACTCTTAGCAACGGATATCTAGGCTCTCGTATCGATGAAGAACGCAGCG302910.10574882094306332No Hit
GCCGCGGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA286870.10014910126419256RNA PCR Primer, Index 9 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCC386350.043.9486414
GTATGCC4817100.043.8963445
TAGTATA225550.043.7084668
GTATAGT225450.043.4684610
TATAGTG226000.043.38257611
TCGTTGT206850.043.3079152
CGTATGC4883500.043.2966444
TCGTATG4890700.043.2729143
CGTTGTA332400.043.2237973
ATCTCGT4915450.042.94727740
GTATTCC224550.042.84134722
CTCGTAT4937500.042.8044442
TCTCGTA4931800.042.78002541
AGTATAG229100.042.7268379
TGTAGTA227050.042.616676
GTAGTAT231450.042.4679377
TATTCCC227300.042.3725123
GATCTCG4868450.042.33474739
TTCCCGC227600.042.23759525
TCTTAGC75950.042.073785
Produced by FastQC (version 0.11.2)