FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l02n01_smallrna_salt24h_rep1_root.3510000006c1bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l02n01_smallrna_salt24h_rep1_root.3510000006c1bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16105241
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC361534422.448245263762274RNA PCR Primer, Index 2 (100% over 51bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA3935542.4436393097129065No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC920180.5713543808503083No Hit
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT728700.4524614068178179RNA PCR Primer, Index 1 (100% over 30bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTTGGAATTCTCGGGTG527170.32732822812151646No Hit
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT439460.27286769567745056RNA PCR Primer, Index 2 (100% over 39bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC432740.268695140917171RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTCGTATGCC411780.25568074392677514RNA PCR Primer, Index 2 (98% over 51bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA403750.25069478935459577No Hit
GGCGAGCACCGACCTGCTCGACCCTTCTGCCGGCGATGCGCTCCTGGCCTT388840.2414369334802255No Hit
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT378250.23486143423746345RNA PCR Primer, Index 2 (100% over 39bp)
GCCCCCGACGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA367390.2281182877052259RNA PCR Primer, Index 2 (100% over 36bp)
GGCTGACGCGGGCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG357920.22223821425584378RNA PCR Primer, Index 2 (100% over 35bp)
GAGAATCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA343090.21303003165242915RNA PCR Primer, Index 2 (100% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG316510.19652608737739471RNA PCR Primer, Index 2 (100% over 35bp)
CGCCGTCCGAATTGTAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA308550.19158359691730165RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGCATCTCGTATGCC307890.19117379243191704RNA PCR Primer, Index 2 (98% over 51bp)
CAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTC306210.19013065374184715No Hit
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT302320.18771529094162578RNA PCR Primer, Index 2 (100% over 39bp)
GCCGGCGGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG300730.18672803468138105RNA PCR Primer, Index 2 (100% over 38bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCG279920.17380677507402714RNA PCR Primer, Index 2 (98% over 51bp)
CGCCGTCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCC265750.16500839695599712No Hit
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA262900.1632387866782RNA PCR Primer, Index 2 (100% over 40bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGACGTATCTCGTATGCC249890.15516067098902772RNA PCR Primer, Index 2 (98% over 51bp)
CAACTGTTAACAATGGATATCTTGGCTCTTATAACGATGAAGAACGCAGCA228350.14178614278420298No Hit
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG221430.1374894048465341RNA PCR Primer, Index 2 (100% over 35bp)
TTGAGAATCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC209860.13030540803456464RNA PCR Primer, Index 2 (100% over 34bp)
GGGCGGCGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT203400.12629429140489112RNA PCR Primer, Index 2 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA202300.1256112839292501RNA PCR Primer, Index 2 (100% over 36bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC198320.12314003869920358RNA PCR Primer, Index 1 (100% over 33bp)
GCCCCGTCCGGCCCGGACCCTGTCGCCTGGAATTCTCGGGTGCCAAGGAAC198140.12302827383955323RNA PCR Primer, Index 1 (100% over 24bp)
GTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCCA197110.12238873047599848No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG192140.11930277851787503RNA PCR Primer, Index 2 (100% over 38bp)
CTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC189490.11765735141746714RNA PCR Primer, Index 2 (100% over 42bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC188870.1172723835675604RNA PCR Primer, Index 2 (98% over 51bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGGAATTCTCGGGTGCCA180090.11182074208017129No Hit
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT179560.11149165666008971RNA PCR Primer, Index 2 (100% over 39bp)
CCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA177140.1099890402136795RNA PCR Primer, Index 2 (100% over 40bp)
TGCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATT166070.10311550134518323No Hit
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA163090.10126517200208306RNA PCR Primer, Index 2 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT447650.044.7101022
TAGTATA489250.044.6721348
GTATAGT487650.044.56502510
TGTAGTA487250.044.5627636
AGTATAG487950.044.5560659
TATAGTG488150.044.52398311
CGTTGTA476000.044.492323
GTAGTAT491400.044.490417
TATTCCC489200.044.4054323
GTTGTAG483300.044.3516854
TTCCCGC489150.044.29041325
GTATTCC490350.044.28293622
TCCCGCC492150.044.0204326
GTATGCC4209500.043.81170745
TGAGTAT497600.043.63362519
GAGTATT497450.043.6106120
GTCGTTG458950.043.558481
AGTATTC499700.043.50427221
ATAGTGG503700.043.43524612
CCCGCCT496000.043.23433327
Produced by FastQC (version 0.11.2)