H3NGHBCXY_l02n01_smallrna_heat_1h_rep3_root.3510000006c21e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l02n01_smallrna_heat_1h_rep3_root.3510000006c21e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24816909
Sequences flagged as poor quality	0
Sequence length	51
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	15231449	61.37528650324664	RNA PCR Primer, Index 11 (100% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCCACATCTCGTATGCC	78449	0.31611108377759695	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	72492	0.2921072886232528	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCTACATCTCGTATGCC	70976	0.28599855042382594	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACTACATCTCGTATGCC	55159	0.22226377990909343	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCTACATCTCGTATGCC	48796	0.19662400341638034	RNA PCR Primer, Index 11 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	44743	0.1802923966074905	RNA PCR Primer, Index 11 (98% over 51bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	40961	0.16505278719440847	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTACATCTCGTATGCC	38066	0.15338735375948712	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGATACATCTCGTATGCC	37100	0.14949484643716104	RNA PCR Primer, Index 11 (98% over 51bp)
TGGANTTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	33685	0.1357340674457081	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	32850	0.13236942602320054	RNA PCR Primer, Index 11 (97% over 36bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	32427	0.13066494300317577	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGGCTACATCTCGTATGCCG	29068	0.11712981661011852	TruSeq Adapter, Index 11 (96% over 33bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	27834	0.11215740042404153	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAATCTCGTATGCCG	26926	0.10849860472148243	RNA PCR Primer, Index 11 (97% over 38bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	25259	0.1017814104085243	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTGCATCTCGTATGCC	25187	0.10149128563915837	RNA PCR Primer, Index 11 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CTATATC	3490	44.146828	36
GCTATAT	3200	44.14119	35
ACGACTA	6770	44.05395	32
GTATGCC	1714760	44.050667	45
TCACTGC	11050	43.925747	30
AGTCACT	18205	43.84755	28
TGCATCT	5930	43.84676	37
CATCTCG	1707035	43.832962	39
TCTCGTA	1723645	43.80719	41
ATCTCGT	1725105	43.800526	40
ACTACAT	7455	43.77782	35
CGTATGC	1725970	43.76247	44
TCGTATG	1727720	43.760197	43
CTCGTAT	1727130	43.757343	42
TCACGAC	10805	43.735218	30
ACATCTC	1698360	43.71212	38
TCTACAT	6870	43.675056	35
CCACATC	13515	43.653255	36
GTCACGA	13030	43.60326	29
AGTTCTC	10230	43.56329	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content