H3NGHBCXY_l02n01_smallrna_heat_1h_rep2_root.3510000006c1f3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l02n01_smallrna_heat_1h_rep2_root.3510000006c1f3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20318945
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC	8753097	43.078501369042534	RNA PCR Primer, Index 10 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	149526	0.7358945063338672	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCATATCTCGTATGCC	59855	0.2945773021187862	RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTCATCTCGTATGCC	57024	0.2806444921229916	RNA PCR Primer, Index 10 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	39347	0.19364686503162443	RNA PCR Primer, Index 3 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCGTATCTCGTATGCC	35505	0.17473840300271495	RNA PCR Primer, Index 10 (98% over 51bp)
GGGAGCTGGCAAATAAGCTTTGATCCGGAGATTTCTGAATGGGGAAACCCA	32669	0.16078098543009983	No Hit
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT	29556	0.14546030810162633	RNA PCR Primer, Index 10 (100% over 43bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT	28098	0.13828473870075442	RNA PCR Primer, Index 10 (100% over 39bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	27627	0.13596670496425872	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACCTTATCTCGTATGCC	25717	0.1265666106188092	RNA PCR Primer, Index 10 (98% over 51bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT	24025	0.11823940662273559	RNA PCR Primer, Index 10 (100% over 43bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA	22934	0.1128700333604919	RNA PCR Primer, Index 10 (100% over 40bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT	21204	0.1043558117805821	RNA PCR Primer, Index 10 (100% over 39bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT	21160	0.10413926510456127	RNA PCR Primer, Index 10 (100% over 39bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC	20902	0.102869514140621	RNA PCR Primer, Index 10 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	981550	44.35326	45
CGTATGC	990105	43.96641	44
TCGTATG	992365	43.91501	43
TCTCGTA	990880	43.914886	41
CTCGTAT	992505	43.89103	42
ATCTCGT	992320	43.89007	40
CATTCCC	17510	43.803104	14
CGTATCT	6020	43.75256	37
TTATCTC	964630	43.416603	38
TCACTAC	5890	43.381012	30
CATCTCG	13915	43.24022	39
TATCTCG	988180	43.228992	39
CTAGCTC	6260	42.973415	33
CTAGCAT	6570	42.828705	33
CTTATCT	976375	42.772614	37
CATATCT	9300	42.760456	37
GCATATC	7700	42.73627	36
CACTACC	5485	42.56546	31
GTCACTT	3315	42.542267	29
GTCACGA	5460	42.471992	29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content