H3NGHBCXY_l02n01_smallrna_drought_1h_rep2_root.3510000006c26e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l02n01_smallrna_drought_1h_rep2_root.3510000006c26e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20407349
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	4884711	23.93603892401703	RNA PCR Primer, Index 3 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	252359	1.2366084394401253	RNA PCR Primer, Index 1 (100% over 30bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT	130658	0.6402497453245887	No Hit
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	94572	0.46342129004605154	RNA PCR Primer, Index 3 (100% over 34bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	88664	0.43447093495583383	RNA PCR Primer, Index 1 (100% over 30bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC	61983	0.30372881847612837	No Hit
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC	46277	0.22676634775050888	RNA PCR Primer, Index 3 (100% over 39bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC	43655	0.21391803511568308	RNA PCR Primer, Index 3 (100% over 42bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC	43630	0.21379553022786058	RNA PCR Primer, Index 3 (100% over 39bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT	43074	0.21107102152268772	RNA PCR Primer, Index 3 (100% over 41bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTACGCATCTCGTATGCC	37200	0.1822872730799086	RNA PCR Primer, Index 3 (98% over 51bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT	35820	0.17552500327210555	RNA PCR Primer, Index 3 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTATGCATCTCGTATGCC	33754	0.16540119934245257	RNA PCR Primer, Index 3 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	32462	0.15907014673978478	RNA PCR Primer, Index 1 (100% over 33bp)
AAGGGTGCTGAGAATACTTTAATCTGACATGGAATTCTCGGGTGCCAAGGA	31162	0.15269989257301375	RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCGGCATCTCGTATGCC	30147	0.1477261941274195	RNA PCR Primer, Index 3 (98% over 51bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT	28425	0.13928805745420436	RNA PCR Primer, Index 1 (97% over 35bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC	24683	0.12095152584492969	RNA PCR Primer, Index 3 (100% over 39bp)
CCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATT	24395	0.11954026953721426	No Hit
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT	24048	0.1178399016942377	RNA PCR Primer, Index 3 (100% over 43bp)
CCGTGTGAAAGTAGGACATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAA	21955	0.1075837924857364	RNA PCR Primer, Index 1 (100% over 23bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA	20686	0.1013654443798653	RNA PCR Primer, Index 3 (100% over 40bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGGCATCTCGTATGCC	20650	0.10118903734140088	RNA PCR Primer, Index 3 (98% over 51bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC	20439	0.10015509608817881	RNA PCR Primer, Index 3 (100% over 39bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	558885	44.471222	45
CATTCCC	29010	44.06266	14
CGTATGC	564185	44.051537	44
TCTCGTA	563745	44.032024	41
CTCGTAT	564880	44.017616	42
TCGTATG	565565	43.99456	43
ATCTCGT	565365	43.929367	40
CATTCCT	10475	43.80464	16
CATCTCG	564485	43.51016	39
TACGCAT	3930	43.49768	35
TTACGCA	3840	43.052776	34
TATGCAT	4480	42.92726	35
CACACGA	6015	42.87581	1
GCATCTC	566465	42.560345	38
ATGCATC	5425	42.456604	36
TAATGCA	1665	42.391	4
AGCGCAG	6340	42.18261	9
CACTTAC	5750	42.09061	31
TGCATCT	6815	41.65226	37
CGTTGTA	8230	41.620045	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content