H3NGHBCXY_l02n01_smallrna_drought_1h_rep1_root.3510000006c245.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l02n01_smallrna_drought_1h_rep1_root.3510000006c245.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7910674
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	3256245	41.162674634297915	RNA PCR Primer, Index 1 (100% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCCCGATCTCGTATGCC	42616	0.5387151587841946	RNA PCR Primer, Index 1 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	37032	0.4681269889266072	RNA PCR Primer, Index 1 (100% over 34bp)
AAGGCTCCGATCGGTAGCTGGTCTGAGAGGATGACCAGCCACACTGGGACT	20920	0.26445281400800996	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACCATCTCGTATGCC	19425	0.24555429790179698	RNA PCR Primer, Index 1 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19124	0.2417493123847601	RNA PCR Primer, Index 1 (100% over 33bp)
CTGAAGGGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTGTGGAATGGAA	18700	0.23638946567637598	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCTCGATCTCGTATGCC	17746	0.22432981058251167	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCACGATCTCGTATGCC	16175	0.20447056723611667	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	16112	0.20367417491859732	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAAGATCTCGTATGCC	13558	0.17138868318932116	RNA PCR Primer, Index 1 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12579	0.15901299939802854	RNA PCR Primer, Index 1 (100% over 30bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG	11922	0.15070776522961254	RNA PCR Primer, Index 1 (100% over 39bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	11775	0.14884951648873407	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAACGATCTCGTATGCC	10720	0.1355131054572594	RNA PCR Primer, Index 1 (98% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA	9460	0.11958525910687257	RNA PCR Primer, Index 1 (100% over 37bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT	9336	0.11801775676762813	RNA PCR Primer, Index 1 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAACACGATCTCGTATGCC	8294	0.10484568065881618	RNA PCR Primer, Index 1 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	7929	0.10023166167636285	RNA PCR Primer, Index 1 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	375885	44.261097	45
TCTCGTA	377575	43.955475	41
CGTATGC	378590	43.95138	44
ATCTCGT	377920	43.943077	40
TCGTATG	379240	43.928562	43
CTCGTAT	378690	43.893764	42
GATCTCG	371865	43.76906	39
TCACAGC	3705	43.71065	30
CGATCTC	369905	43.468906	38
CTATCTC	1715	43.15049	38
TCCCGAT	5020	42.83565	35
ATCACTA	580	42.659515	34
TCTCGAT	2305	42.644375	35
TCACTAT	670	42.63636	35
ACGATCT	367485	42.588253	37
CACAGCT	1100	42.531788	31
CACGATC	367650	42.307896	36
GTCACAG	3995	42.283035	29
TGCGTTA	730	42.212463	7
CATTCCC	1540	42.21046	14

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content