FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l01n02_smallrna_heat_1h_rep1_root.3520000006c25f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l01n02_smallrna_heat_1h_rep1_root.3520000006c25f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9440577
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT457634048.475215021285244Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1316751.3947770353443438Illumina RNA PCR Primer (100% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC466840.49450367281576113Illumina RNA PCR Primer (100% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT375980.39825955553352305Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT322200.3412926985289141Illumina RNA PCR Primer (97% over 46bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT281370.2980432234173822Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAAATCTCGGTGGTCGCCGTAT255020.27013179385115976Illumina RNA PCR Primer (97% over 46bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG184880.19583548759784494Illumina RNA PCR Primer (100% over 34bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA169100.17912040757678266Illumina RNA PCR Primer (100% over 40bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC164320.1740571577351681Illumina RNA PCR Primer (97% over 45bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG148200.15698193023583198Illumina RNA PCR Primer (100% over 34bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC147880.15664296790333898Illumina RNA PCR Primer (97% over 47bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA144550.15311564113083342No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG143890.15241653132006655Illumina RNA PCR Primer (100% over 34bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT131210.13898514889503047Illumina RNA PCR Primer (97% over 46bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT129280.1369407823271819Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT123420.1307335346134034Illumina RNA PCR Primer (100% over 38bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC121070.12824427998415774No Hit
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT120060.12717443012222662Illumina RNA PCR Primer (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT5030350.044.18485345
CCGTATC111250.043.18829745
TGTACAT32700.043.00238428
ATAACCT14950.042.5805410
CGTGTAC33000.042.47734526
CGCCGTA5269850.042.2942744
TCGCCGT5272650.042.23809443
GGTCGCC5277300.042.19896741
ACCGTAT15100.042.18623745
TGGTCGC5277900.042.14920440
GTCGCCG5301850.042.0871742
GTGGTCG5295550.042.0122839
GTACATC33500.041.97612829
GGAATAC17600.041.9205676
GATAACC15250.041.8903929
CGATAAC15150.041.869958
TGTATAT33150.041.8078128
GGTGGTC5351600.041.69209338
CGTGTAT33450.041.4350526
ACGATGT32450.041.244717
Produced by FastQC (version 0.11.2)