H3NGHBCXY_l01n02_smallrna_ctrl1h_rep3_root.3520000006c235.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n02_smallrna_ctrl1h_rep3_root.3520000006c235.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6270489
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	3905217	62.27930548957187	Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	84984	1.3553009980561324	Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAAATCTCGGTGGTCGCCGTAT	22176	0.3536566286935517	Illumina RNA PCR Primer (97% over 46bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	22120	0.35276355639887097	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	21354	0.340547603225203	Illumina RNA PCR Primer (97% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	18098	0.28862182837734024	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	13041	0.20797421062376475	Illumina RNA PCR Primer (97% over 46bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAN	12715	0.20277525405115931	Illumina RNA PCR Primer (97% over 45bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	12391	0.19760819291764964	Illumina RNA PCR Primer (97% over 46bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	10348	0.16502700188135247	Illumina RNA PCR Primer (100% over 40bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	8250	0.1315686862699225	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	6997	0.11158619367644214	No Hit
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	6987	0.11142671648096344	Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	6911	0.11021468979532537	Illumina RNA PCR Primer (100% over 34bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	6895	0.10995952628255948	Illumina RNA PCR Primer (100% over 38bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	6787	0.10823717257138957	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	6744	0.10755142063083119	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGACGTAT	6441	0.10271926160782675	Illumina RNA PCR Primer (97% over 46bp)
CGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	6283	0.1001995219192634	Illumina DpnII expression Sequencing Primer (96% over 30bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	423850	44.722206	45
ACCGTAT	1345	43.67572	45
TGTACAT	2640	43.292477	28
GGAATAC	915	43.26812	6
CGCCGTA	440165	43.179157	44
TCGCCGT	440195	43.147594	43
TGTATAT	2550	43.14409	28
GGTCGCC	440615	43.08417	41
TGGTCGC	440400	43.06331	40
GTGGTCG	441190	43.000977	39
GTCGCCG	443015	42.95996	42
TATATCT	2590	42.913136	30
ATATCTC	2600	42.83564	31
GGTGGTC	444580	42.81631	38
GTATATC	2595	42.743748	29
GTACATC	2685	42.735516	29
TATCTCG	2580	42.731663	32
CGTGTAC	2680	42.564068	26
CGGTGGT	447905	42.513714	37
GTGGTCA	1340	42.480453	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content