FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l01n02_smallrna_ctrl1h_rep2_root.3520000006c228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l01n02_smallrna_ctrl1h_rep2_root.3520000006c228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11322459
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT690904261.02068464103072Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1473701.3015723881181642Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTACATCTCGGTGGTCGCCGTAT450750.3981025676489533Illumina RNA PCR Primer (97% over 46bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT397910.35143425999599553Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAAATCTCGGTGGTCGCCGTAT389230.34376808076761417Illumina RNA PCR Primer (97% over 46bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT368820.3257419611764547Illumina RNA PCR Primer (97% over 46bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT214640.1895701278317722Illumina RNA PCR Primer (97% over 46bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG198470.1752887778176101Illumina RNA PCR Primer (100% over 34bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT188520.16650093411687336Illumina RNA PCR Primer (97% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT174850.1544275850325446Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA149340.1318971435445251Illumina RNA PCR Primer (100% over 40bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA148160.13085496710564373Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT139350.12307397182891103Illumina RNA PCR Primer (100% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT7467600.044.8197545
TGTATAT48400.043.60228328
TATCTCG48700.043.56756632
GTATATC48700.043.42704429
TGTACAT48150.043.36141628
CGCCGTA7744150.043.33363744
TCGCCGT7742750.043.29788643
GGTCGCC7745200.043.26123441
TATATCT49050.043.25477630
TGGTCGC7743250.043.22999240
GTGGTCG7749850.043.17539
GTGTATA48850.043.1545727
GTCGCCG7783850.043.14267742
CGTGTAT49150.043.0763626
GGTGGTC7801700.043.0264938
CGTGTAC48450.042.95570426
CGGTGGT7855200.042.75119437
ACCGTAT22850.042.70070645
GTACATC49050.042.65847429
TCGGTGG7919650.042.42260436
Produced by FastQC (version 0.11.2)