H3NGHBCXY_l01n02_smallrna_ctrl1h_rep1_root.3520000006c20e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n02_smallrna_ctrl1h_rep1_root.3520000006c20e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8726571
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	4835885	55.415638055314055	Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	109362	1.2532070156766042	Illumina RNA PCR Primer (100% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	55510	0.6361032300086712	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	27004	0.30944571470283116	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	19352	0.22175949751626384	Illumina RNA PCR Primer (97% over 46bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	18799	0.21542252965110809	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	17257	0.19775235885893783	Illumina RNA PCR Primer (97% over 46bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	16727	0.19167895385254988	Illumina RNA PCR Primer (97% over 46bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	14476	0.1658841714574946	Illumina RNA PCR Primer (100% over 34bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	13076	0.14984121483684715	No Hit
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	10990	0.12593720947208245	Illumina RNA PCR Primer (100% over 38bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	9633	0.11038700080478346	Illumina DpnII expression Sequencing Primer (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	525035	44.742332	45
TGTACAT	3570	43.168446	28
CGTGTAC	3560	43.165287	26
CGCCGTA	546280	43.14001	44
TCGCCGT	546215	43.102722	43
GGTCGCC	546935	43.046803	41
TGGTCGC	546720	43.02175	40
GTACATC	3600	42.996925	29
GTGGTCG	547780	42.937107	39
GTCGCCG	549740	42.927025	42
ACCGTAT	1740	42.85455	45
GGTGGTC	552370	42.717175	38
GGGAATG	7100	42.673054	2
TGTATAT	3340	42.571075	28
CGTGTAT	3335	42.569393	26
GTGGTCA	1755	42.56155	39
GTATATC	3345	42.508175	29
TATCTCG	3335	42.43495	32
TATATCT	3365	42.389244	30
CGGTGGT	558150	42.27723	37