H3NGHBCXY_l01n02_smallrna_ctrl025h_rep3_root.3520000006c1e3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n02_smallrna_ctrl025h_rep3_root.3520000006c1e3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11120743
Sequences flagged as poor quality	0
Sequence length	51
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	2309087	20.763783498998222	Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	228468	2.054431075333725	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	75397	0.6779852749047433	Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	70309	0.6322329362345663	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	67771	0.6094107201290417	Illumina RNA PCR Primer (100% over 35bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	60944	0.5480209370902646	Illumina RNA PCR Primer (100% over 35bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT	56974	0.5123218835288255	No Hit
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	54242	0.48775517966740173	No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	50136	0.4508331862358477	Illumina RNA PCR Primer (100% over 45bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	42303	0.380397245040192	Illumina RNA PCR Primer (100% over 33bp)
GGCTGCGCCGACGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT	37920	0.34098441084377185	Illumina RNA PCR Primer (100% over 31bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	36756	0.33051748430837763	Illumina RNA PCR Primer (100% over 34bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG	33635	0.3024528127302285	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	33541	0.301607545467061	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	31168	0.28026904317454326	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	30201	0.27157358100983	Illumina RNA PCR Primer (100% over 38bp)
GCCCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	29386	0.26424493399406856	Illumina RNA PCR Primer (100% over 37bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	26352	0.23696258424459588	No Hit
GCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	25860	0.23253841942035705	Illumina RNA PCR Primer (100% over 34bp)
GCCCGCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	25799	0.2319898949197909	Illumina RNA PCR Primer (100% over 36bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	24391	0.21932887038213184	Illumina RNA PCR Primer (100% over 34bp)
TCGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	24236	0.2179350786183981	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	24042	0.21619059086249903	Illumina RNA PCR Primer (100% over 36bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	23634	0.21252177125215466	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC	22702	0.20414103625989738	No Hit
GCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	22168	0.19933919882871134	Illumina RNA PCR Primer (100% over 32bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	21993	0.1977655629664313	Illumina RNA PCR Primer (100% over 37bp)
CGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	21795	0.19598510639082298	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA	21003	0.18886328008838976	No Hit
GGCCGGCCGCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	20472	0.1840884192719857	Illumina RNA PCR Primer (100% over 33bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	19601	0.17625620878029463	Illumina RNA PCR Primer (100% over 34bp)
CCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT	19244	0.1730459916212433	Illumina RNA PCR Primer (100% over 31bp)
AGGGATGCAACACGAGGACTTCCCAGGAGGTCACCCATCCTAGTACTACTC	19149	0.1721917321531484	No Hit
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	19055	0.17134646488998082	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT	18725	0.168379037263967	Illumina RNA PCR Primer (100% over 31bp)
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	18719	0.1683250840344031	Illumina RNA PCR Primer (100% over 35bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	18369	0.16517781230984296	Illumina RNA PCR Primer (100% over 34bp)
GGCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	17721	0.15935086351694308	Illumina RNA PCR Primer (100% over 36bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	17625	0.15848761184392085	Illumina RNA PCR Primer (100% over 38bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	17104	0.1538026730767899	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	15621	0.14046723316958226	No Hit
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	14841	0.13345331332627686	Illumina RNA PCR Primer (100% over 35bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC	14665	0.13187068525906948	No Hit
GCCCCCGTCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	14638	0.13162789572603198	Illumina RNA PCR Primer (100% over 32bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	14320	0.12876837455914592	Illumina RNA PCR Primer (100% over 38bp)
GCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	13714	0.12331909837319324	Illumina RNA PCR Primer (100% over 36bp)
GCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	13198	0.11867912063069887	Illumina RNA PCR Primer (100% over 37bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAAATCTCGGTGGTCGCCGTAT	12808	0.11517216070904615	Illumina RNA PCR Primer (97% over 46bp)
AGGCCAGGAGCGCATCGCCGGCAGAAGGGTCGAGCAGGTCGGTGCTCGCCG	12417	0.11165620858246611	No Hit
CCGGCTGCGCCGACGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	12217	0.10985776759700318	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	12032	0.10819420968544997	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	12026	0.10814025645588608	Illumina RNA PCR Primer (100% over 37bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	11930	0.10727700478286388	Illumina RNA PCR Primer (100% over 37bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	11436	0.10283485554877043	Illumina DpnII expression Sequencing Primer (96% over 29bp)

Adapter Content

Adapter graph

Kmer Content

$Kmer graph$

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	252420	44.63777	45
GGGAATG	28130	43.71391	2
ATAACCT	4130	43.627647	10
TAACCTA	4145	43.198425	11
CGCCGTA	262440	43.081696	44
GGTCGCC	263565	42.88605	41
GATAACC	4270	42.829403	9
TCGCCGT	264215	42.75821	43
TGGTCGC	264150	42.7323	40
GTGGTCG	266565	42.34467	39
GTGACGC	4660	42.283432	3
ACTATAC	7645	42.198395	18
GTCGCCG	268785	42.130184	42
ACGATAA	4335	42.083427	7
CACTATA	7655	42.055103	17
GAATACT	7495	42.01804	7
CGATAAC	4345	41.934803	8
GGAATAC	7530	41.822735	6
TACTCAC	7690	41.74236	10
TATACTA	7785	41.675774	20

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content