FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l01n02_smallrna_ctrl025h_rep2_root.3520000006c1c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l01n02_smallrna_ctrl025h_rep2_root.3520000006c1c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8842507
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT309025334.94770204875156Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA664900.75193607423777Illumina RNA PCR Primer (100% over 45bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT627940.7101379733145815Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT420910.4760075394907802Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT395690.44748621629589885Illumina RNA PCR Primer (100% over 35bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG393660.4451904872679207Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG377380.42677941900413535Illumina RNA PCR Primer (100% over 34bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC356420.4030757340650112Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT315530.356833192215737Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT307080.3472770787741531Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG291330.3294653880398399Illumina RNA PCR Primer (100% over 36bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG257520.2912296252635141Illumina RNA PCR Primer (100% over 37bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA210290.23781717108055442No Hit
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG202120.2285777099186916Illumina RNA PCR Primer (100% over 34bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT195640.22124947144514562Illumina RNA PCR Primer (100% over 38bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA194290.21972275509649017Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG184870.20906966768587235Illumina RNA PCR Primer (100% over 34bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT172130.19466198895856118Illumina RNA PCR Primer (97% over 46bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG160060.18101201390058272Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC151120.1709017589694868Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG146460.1656317603141281Illumina RNA PCR Primer (100% over 37bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG142840.16153789869773358Illumina RNA PCR Primer (100% over 33bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG136180.15400609804436682Illumina RNA PCR Primer (100% over 37bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC130200.14724331007032282Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG107990.12212599888244363Illumina RNA PCR Primer (100% over 40bp)
TCGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC104550.11823569944586981Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT98210.11106578711218436Illumina RNA PCR Primer (100% over 31bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT97280.11001404918311063Illumina RNA PCR Primer (97% over 46bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG94590.10697192549578984Illumina RNA PCR Primer (100% over 34bp)
GGCCGGCCGCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG94170.10649694707620815Illumina RNA PCR Primer (100% over 33bp)
CCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT90280.10209774219008252Illumina RNA PCR Primer (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT3332050.044.73462345
CGCCGTA3467450.043.13796244
TCGCCGT3471750.043.0586143
GGTCGCC3477800.042.96890641
ATAACCT20000.042.9648310
TGGTCGC3481100.042.8758240
GTCGCCG3508400.042.69482442
GTGGTCG3512800.042.4630439
GATAACC20900.042.190979
CGATAAC21400.042.0461248
TAACCTA19700.042.0205111
GGGAATG53900.041.981172
ACGATAA21550.041.9622277
ACTGACA11100.041.7735671
GGTGGTC3610000.041.46095338
AATTGAC1250.041.3972328
CATATGC10600.041.3842916
GGAATAC27850.041.1931236
GACGATA21900.041.1888856
CACTATA27850.041.1156117
Produced by FastQC (version 0.11.2)